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We narrowed to 749 results for: Tro;

Showing: 361 - 390 of 749 results

  1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ... Martin et al., 2018). The ACE reporter is a bicistronic construct that consists of a mutated mCherry ...eGFP. To create the inactive mCherry, the lab introduced a 43 base-pair insertion that disrupts fluorescence...use across different cell types and systems. To introduce flexibility, the Dow lab created a base editing... optimized cytidine base editor. The lab then introduced an sgRNA targeting the GFPACG to the cells. As...

  2. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...overexpression vector rather than going through all the troubleshooting required for Cas9-based activation. CRISPRa...Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ (2019...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11...

  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...founder of Research Space. There are many types of electronic lab notebooks (ELNs), each with its own pros ... virtue of liberating data from paper into an electronic environment and hence making it searchable and... of Research Space, which provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts...on the Addgene blog Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium...

  4. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...social sciences go uncited. Creative titles and controversial topics can garner some attention, though in ...Journal of Number Theory (1,519 views), and Gastroenterology (1,046 views) rounded out the top four average...infections. The video abstracts provide a brief introduction to the research and, after watching the video...links to the videos, keeping the copyright and control over the videos with the researcher and the host...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing Following...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...transient transfections and are handy for quick in vitro experiments. shRNA plasmids typically use RNA polymerase...common promoters are U6, H1, and 7SK. These are strong promoters with no tissue specificity, so in some...

  6. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...neither the aptamer nor the small molecule are strongly fluorescent but the binding of an aptamer to its...plasmids encoding aptamer-tagged RNA and later introducing the small molecule into the cell. The fluorescence...metabolites, leading to conformational changes that control the expression of coding RNA. Spinach-based riboswitches...then identified through microfluidic-assisted in vitro compartmentalisation for their high binding affinity...

  7. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...Level 1 microbe makes it a perfect candidate for introductory teaching and research environments (Idalia &...study human cardiovascular disease, specifically stroke and hypertension (Iannaccone et al., 2009). Rodents...Resource, a breeding facility for X. laevis and X. tropicalis, the two most commonly used species of frog. ... study human disease-relevant genes in such a controlled environment displays how essential the frog is...

  8. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...reviewer’s concerns, you are making your hypothesis stronger. When you share data at a conference poster session... can frequently be traced back to one of a few trouble spots:  Communication (or lack thereof): Information...Empathizing with people, especially people with whom you strongly disagree, is a critical component of conflict ...tools I’m mentioning here, this may be the most controversial, so I would like to qualify it. If you are attempting...

  9. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...most important ways to spatially and temporally control gene expression, especially in in vivo models, ...Golgi staining, injection of diffusible labels, or electron microscopy of stained sections, were limited due...functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different.... The use of AAV provides spatial and temporal control without the need for germline modification, and...

  10. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ...priorities and needs, but sometimes it just takes introspective thought on what’s worked for you and not worked...reputation? If you are choosing a training lab, I strongly suggest you complete a work rotation in the lab... it could also be a sign that the lab head has trouble attracting scientists. To get a better idea of ...and non-academic career paths. I can’t say this strongly enough - don’t choose a mentor that sees you only...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...as the insertion of a fluorescent tag or the introduction of a specific mutation, one generally relies...one but two rounds of single cell cloning – as a control, one should revert the edit back to the original...technologies offer an alternative to HDR for introducing edits. The same locational constraints are even...the method of producing the guide (synthetic, in vitro transcription, or lentiviral delivery) can affect...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...formation in the genome and are inefficient at introducing point mutations. In 2016, the researchers at ... Broad Institute envisioned a new strategy to introduce point mutations in the genome. They fused a catalytically...base editors (CBE) use a cytidine deaminase to introduce a C→T change at precise locations in the genome...his super giant albino gorilla fighting off a monstrous wolf and crocodile, that were “affected by CRISPR...

  13. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...Correlative light and electron microscopy (CLEM) is an imaging technique that combines electron microscopy (EM...cellular structures (0.5-1% osmium tetroxide). These conditions destroy most PA-FPs, but, in 2015, the Looger...

  14. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...researcher in the University of Michigan Department of Astronomy. She studies how planets form around young stars...community of science supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is ... an agenda that halts current data collection, destroys existing data, and guts future funding.I marched...march read "You know things are serious when the introverts arrive." I couldn’t watch the denial of facts...

  15. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...initiate our MTA process and perform standard quality control but will not make the plasmids available until:...Continue to Next Step". As part of our quality control process, we will sequence the full plasmid and ...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the NEB Stable...received them and they will undergo our quality control process to verify your plasmids. Our friendly Addgene...

  16. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ... Using the FITS Approach Caitlin Faulds, who introduced the very first antibody deposit (the Integrin...flow cytometry expertise in three posts. An Introduction to Gating in Flow Cytometry, Designing Your ...two posts. Flow Compensation and Flow Cytometry Controls Priyamvada Prathima, who wrote a helpful guide...

  17. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...Podcast breakdown (Time : Topic) 0:00 - 2:00 : Introduction to the podcast from producers Tyler Ford and...and Eddy Page. 2:00 - 3:55 : Alex introduces himself and explains why he decided to do an MD PhD - it's...in generating Cas9 based tools to enable facile control of DNA and RNA.     Special thanks to our sound...

  18. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...ultimate prize – a glorious trophy. Sure it’s plastic and you have to share 1 trophy among 6 people, but you...occurring earlier but that the photo evidence was destroyed to protect the innocent revelers. Our Halloween...

  19. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ...AAV9 tdTomato virus in the mPFC.  The AAV9 is so strong that you can even see dendritic spines!  Curious...Images that clearly show viral spread and cellular tropism* (min. 300 dpi, PNG format) At least two replicates... the target region, if the report describes a retrograde serotype An abstract that summarizes your experiment...

  20. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    Published
    Sept. 1, 2016, 2:30 p.m.
    ...projects 0:28 - 1:12 : Introduction from Tyler 1:13 - 2:02 : Niroshi and Ben introduce themselves 2:03 - 3...your biggest experimental success? 31:32 - end : Outro In our next episode, Niroshi and Ben discuss how...

  21. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...interview with Addgene's founders Podcast breakdown Intro Chapter 1 - Who are Addgene's Founders? Chapter ...the importance of sharing information/knowledge Outro Many thanks to our guests, Melina Fan, Kenneth ...nonprofit? Get an Inside Look at Addgene's NGS Quality Control Addgene's Viral Service - Why Virus? Why Now? ...

  22. Plasmid Grant to Aid Cervical Cancer Screening in Ghana

    Type
    Blog Post
    Published
    Nov. 8, 2016, 3:30 p.m.
    ...grant to order plasmids she can use as positive controls in PCR-based cervical cancer screens. This third...predominantly HPV 16 and 18.” HPV plasmids as positive controls To start her work, Gladys will improve cervical... containing a variety of HPV genes as positive controls in her PCR screens. She hopes that these screens...

  23. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...parental cell line, but not in the iso-genic knockout control. This can involve cell surface labeling and/or ... Additionally, it can be a real challenge to troubleshoot RNAi failures. Potential explanations for RNAi... target of interest, you can add an additional control containing treated cells. The expected change in...55133-7 More resources from the Addgene blog Introduction to Gating in Flow Cytometry Antibodies 101: ...

  24. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    Published
    Dec. 18, 2018, 1:17 p.m.
    ...the performance of different nucleases. Nicola Patron’s lab compared the efficiency and specificity of...plasmids Anti-CRISPR proteins for optogenetic control of CRISPR-Cas9 Anti-CRISPR proteins that inhibit...has adapted anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus...

  25. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...They kicked off their day with breakfast and an introduction to Addgene with our Executive Director, Joanne...in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology Lianna ...our UK team member Benoit Giquel. Bluegene’s globetrotting impressed our guests, as did how much work is...

  27. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...inactivated the pathway. “The idea is that if you introduce them into cells one at a time, you could identify... for pathways that drive resistance to drugs in vitro, and they’ve shown that it works. But, he says that...

  28. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...growth based on data voluntarily made available by Medtronic via The Yale University Open Data Access (YODA...got a nine percent bump in citations for their trouble, even when all kinds of other factors, including...universities. We particularly recommend Eric Lander's intro to biology course "The Secret of Life." 10)     ...

  29. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...Packaged on Request prep and run the same quality control assays we run with all of our in-stock viral preps...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...

  30. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...McClean’s lab generated an optogenetic toolkit that controls repression of gene expression in Saccharomyces...transcriptional repressor Mxi1, and an optogenetic tool that controls nuclear localization (LINuS). Blue light renders...mRNA decreased after 20 minutes in xylose (which controls expression of dCas9). The lab demonstrated the...
Showing: 361 - 390 of 749 results