...overexpression of protein fusions to gene-edited cells can decrease the number of fluorophores, compromising the photon...improved fluorophores are making chemical dyes increasingly attractive and accessible to cell biologists—and...fluorogenicity, photoactivation etc.—can further increase the photon budget and allow us to push further...

...just might personally hand your credentials to HR, greatly increasing your chances of getting a phone ...can use to better prepare for your career, and increase your chances of landing that next coveted job!...

...researchers in the field. Finally, you can sound credible and cool when you casually mention “Haery et al... al., showed that MYC expression was increased…” when discussing the review topic with your peers. Start...will also paraphrase what I have heard many other creative people say about writing: you don’t know what ...

...prognoses. Survival rates for mesothelioma are creeping upwards, and while most people still die within...world. New mesothelioma cases could be greatly decreased through a ban on asbestos. Nonprofits like the...Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional Resources on Addgene.org ...

...proteins cooperatively can separate the DNA strands. Created with BioRender.com. Once DnaA breaks the local...helicases to continue unwinding the strands. This creates two replication forks that move in opposite directions...pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids under stringent...

...and multiple gRNAs into separate AAV vectors, increasing overall packaging capacity but necessitating ...efficiency of AAV-SaCas9 in vivo, the Zhang lab created an all-in-one SaCas9 and sgRNA construct using ...histologically normal and liver injury markers were not increased compared to a control AAV-GFP. Not only did the...

...1998). As the copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit ...inhibit its own transcription. As copy number increases, RepA transcription is repressed and replication...

...expression transposases leading to increased integration frequency. (C) Increasing rounds of transfer on transposition-inducing...chromosomal integration of cargo without using screening markers. Capability to construct a strain library...

...each project. This creates accountability on the part of each developer and creates trust amongst the ... Addgene grows and evolves, we will be here to increase all of our teams’ efficiency and productivity....

...that can function in more than one host species. Created with BioRender.com. How are shuttle vectors...viral oris to drive amplification, which helps increase cellular uptake, but this approach requires compatible...eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular language...

...made use of mazF as a counterselectable marker to create unmarked genetic modifications in the yeast Pichia...counter selection in one plasmid, they were able to create an efficient cloning tool. The plasmid pKSCTMF ...Morin and James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient...

...glutathione reductase to faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA...salts may be preferable if the protein product is secreted to the medium or if slow expression is required...required due to solubility concerns. Insoluble and Secreted Proteins: The most common purification protocols...

...you need to speak to start creating slides or thinking about content. Create a Google.slides or Powerpoint...capture interesting key results, striking images, creative ideas, and engaging stories as you encounter or...

...percentage of dystrophin-expressing muscle cells increases over time, and all three groups confirm dystrophin...muscle stem cells are modified by this approach, increasing the possibility that edits will persist long-...off-target risk must be evaluated separately, increasing the time to clinical approval. Even with the ...

...should be able to apply someone else's new and incredibly useful technique simply by following the abbreviated...49:45 - 1:18:45), there are also initiatives to create more reliable reagents for future research. I’ve...IPI is working to address this issue directly by creating well characterised antibodies against all human...

...fluorescent dyes, and fluorescent ligands. Figure created with biorender.com. Fluorescence refers to...fluorescent protein tags, such as higher specificity, increased versatility in probe attachment, and compatibility...exciting the fluorophore at peak wavelength can also decrease the rate of photobleaching. Interaction with receptors...

...of antibody validation, and it is recommended to screen multiple candidate antibodies when possible. If...interest. By correlating your data sets, you can increase confidence that the labeling observed represents...experiment. We find it useful to start with a screening procedure in easily genetically modified cell ...

...is a significant hurdle made worse by an ever increasing marketplace. Since 2013 about 11 tools have been...the user is expected to interact with the tool (a screen shot and overview of the table can be found above...re engaged in heavy CRISPR design (I’m talking screens and/or very large genomes), then it is worthwhile...

...for controlling protein activity, methods for increasing CRISPR editing efficiency, new CRISPR base editors...Segment  Hot Plasmid 2: Ouabain selection for increased CRISPR editing efficiency. Listen to the Segement...

...various CRISPR applications (such as visualize, screen, activate, and repress) on Thursday. We’d also ...from nickases to base editing to pooled library screening. It also has information for planning your CRISPR...

...platform changes, such as limiting access and increased priority given to paid content regardless of user...preserve the knowledge, conversations, and community created and shared on our feed, we will not delete @addgene...

...celebrations! We're kicking it off by sharing all the incredible guest posts and stories featured in our blog ...Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene writers High Complexity...

...and Instagram and we’re excited to share these creative entries as the winners of the 2019 contest. ...material tree We often get submissions of decorations created from lab materials. This year, one of the winners...

... guide that curates this information. And so I created the Codon Guide to Synthetic Biology in the hopes...open-access resources whenever possible.  Image credit: Niko McCarty; adapted from Bruce Wetzel and Harry...

...at the University of Amsterdam. In addition to creating and sharing many great fluorescent protein tools...probes and more. Chapter 5 - mTurquoise II and the creation of improved fluorescent proteins through mutagenesis...

...blogger Ava Shipman explains how the Wu lab is screening over 3,000 antibodies for use in mice and human...from 2022! Wow! Our depositors do some really incredible science - I hope you've enjoyed learning about...

...Microbiology Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed...Addgene has so many of these resources that we created filters just for them on our search page. Use the...

...can stage in a lab diorama. Or maybe you want to create a stack of wrapped gifts using life science catalog...Twitter and Instagram shortly after. Have fun, be creative, and enjoy the end of year festivities - however...

...materials into holiday decorations. We appreciated the creative uses of old posters, pipette tip boxes, and more...working on our reports/lab stuff for a few minutes to create this lovely winter scene out of stuff we found ...

...fluorophores other than fluorescent proteins can be incredibly useful in FRET experiments. Professor Fessenden's... also really helped put this technology into a concrete context. I recommend this post to anyone wondering...

...incorporate to reduce your lab consumption and increase your lab sustainability. For my other posts on...Subscribe to the Addgene blog Learn about other creative approaches for lab sustainability Resources on...

...Data reports  Each antibody submission is used to create a data report, which contains experimental data...the experimental conditions.     Figure 4: A screenshot of the materials and methods section for the ...

...complex via the fused HUH endonuclease PCV. This increases HDR efficiency up to 30-fold. Read the paper ...variants with mutations in the rat APOBEC1 that decreases the number of off-target RNA edits by >390 fold...

... doesn’t. Reports receive a DOI for citation, crediting researchers when their data is used by the community...understand our role in opening up science to help create new knowledge and research opportunities. I have...

... affiliated with the recipient organization can create an Addgene account and place an order: a purchasing...order to generate a PO, learn more about how to create a quote. How do I pay the invoice? After your ...

...and energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags) less likely...inntags are indeed innocuous, Georgieva et al. created tagged versions of the cyclin Cdc28 and the chaperone...

...without exclusivity. Chapter 11: The Stem Cell Bank (CReM Bank) at the Center for Regenerative Medicine and... a member of the Addgene board of directors - increasing open source sharing and helping us stick to our...

...compensation committee. The compensation committee creates a compensation philosophy that helps guide employee...rescue organization where I foster dogs and help screen dog adoption applications. My most recent foster...

...into our lab lives. Read on to learn about some creative approaches to lab sustainability, and find ways...learn that it hasn’t even been 50 years since the creation of the United States Environmental Protection ...

... describes an ingenious but simple technique to screen for proteins that could be useful for a wide variety...event gives me the opportunity to dress up as Mr. Incredible, an added bonus. You can find the rest of our...