We narrowed to 608 results for: Sis

1. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of synthetic DNA,...

2. Celebrate National Book Month with Addgene

   Type

   Blog Post

   Published

   Oct. 3, 2019, 12:47 p.m.

   ...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

3. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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   Blog Post

   Published

   Dec. 20, 2018, 1:15 p.m.

   ...report is that when it became clear that this assistive reproductive technique was feasible, three separate...

4. 25 Work From Home Tips for Scientists

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   Blog Post

   Published

   March 19, 2020, 1:15 p.m.

   ...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ...

5. Predicting Adverse Reactions to Monoclonal Antibody Drugs

   Type

   Blog Post

   Published

   Feb. 13, 2024, 2:15 p.m.

   ...3D models of the human lymphatic system. PBMCs consist of various immune cells, and can be used as a whole...

6. K. phaffii: Rising to the Occasion

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   Blog Post

   Published

   April 4, 2023, 1 p.m.

   ...the strain tractable for peroxisomal studies on biogenesis, degradation, and proliferation. Genome manipulation...

7. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   Blog Post

   Published

   Feb. 9, 2021, 2:15 p.m.

   ...industry”. To date, aside from doing science, he has assisted many researchers in gaining the confidence to ...

8. Networking Follow-up: I Met a Person. Now What?

   Type

   Blog Post

   Published

   Jan. 12, 2021, 2:15 p.m.

   ...touch with all of their connections on a regular basis. It is also not at all necessary. Once you’ve talked...

9. What Good Citizenship Can Do for Reproducibility in Science

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   Blog Post

   Published

   Nov. 7, 2019, 2:18 p.m.

   ...found, to providing reagents in an accessible and consistent way so other researchers can use them, to making...

10. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type

    Blog Post

    Published

    March 24, 2016, 2:30 p.m.

    ...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

11. Components of CRISPR/Cas9

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    Blog Post

    Published

    Feb. 2, 2016, 5 p.m.

    ...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

12. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post

    Published

    Aug. 13, 2015, 2:30 p.m.

    ... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

13. Management for Scientists: Delegating is Key

    Type

    Blog Post

    Published

    Feb. 20, 2015, 8:30 p.m.

    ...new measure of success. It is imperative that you resist the temptation to “just do it myself” or micromanage...

14. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...chromatin-related plasmid in a collection that grows on a daily basis, the lists below provide a snapshot of relevant ...

15. Plasmids 101: Inducible Promoters

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    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ...present, YFI is inactive, preventing repressor cI synthesis and allowing reporter gene transcription to take...

16. A Novice's Perspective on Neuroscience - SfN2017

    Type

    Blog Post

    Published

    Dec. 15, 2017, 2:07 p.m.

    ...seem to require some mathematical know-how for analysis. If you’re looking to get into neuroscience and...

17. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

18. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd generation...

19. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...involved in cytoskeletal control, cell growth, and endocytosis. Recently, the Sidhu group at the University ...

20. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...current and future features being built for the analysis software program, and meet with the automation...

21. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ...2008) Pseudotyped Vesicular Stomatitis Virus for Analysis of Virus Entry Mediated by SARS Coronavirus Spike...

22. Hot Plasmids - May 2022

    Type

    Blog Post

    Published

    May 26, 2022, 1:15 p.m.

    ... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...

23. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    Published

    April 5, 2022, 1:20 p.m.

    ...validate your new construct by Sanger sequence analysis. But for this research, it was likely that the...

24. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...data in a manner that demands high accuracy and consistency?  If the answer to any of these questions is...

25. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

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    Jan. 21, 2025, 2:15 p.m.

    ...burdensome task of explaining the steps to a lab assistant. However, I recognized the need to shift my mindset...

26. Viral Vectors 101: Biosensors

    Type

    Blog Post

    Published

    July 6, 2023, 1 p.m.

    ... cellular process. Cellular phenomena such as exocytosis, autophagy, voltage, and tension all have specific...

27. GCE4All: Making Genetic Code Expansion Accessible

    Type

    Blog Post

    Published

    June 15, 2023, 1:15 p.m.

    ...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...

28. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ... for understanding how the brain functions. The basis for these connections in the brain are (drum roll...

29. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type

    Blog Post

    Published

    Aug. 6, 2024, 1:15 p.m.

    ...polypurine tract; recognition site for proviral DNA synthesis. Increases transduction efficiency and transgene...

30. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...reviews on blog posts are done on a case-by-case basis, as many of our blog contributors primarily write...

31. Plasmids 101: Screens vs. Selections

    Type

    Blog Post

    Published

    Nov. 10, 2022, 2:15 p.m.

    ...trying to identify genetic conditions which convey resistance to a drug. You could culture cells in the drug...

32. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...transcription factor. It also gives cells antibiotic resistance to neomycin so that you can select the population...

33. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ... Conference Dinner - by Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships...

34. 3 Challenges in Plant Synthetic Biology

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    Blog Post

    Published

    July 22, 2014, 5:46 p.m.

    ...plant synthetic biology In addition to public resistance, an overall limited understanding of plant biology...

35. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    Published

    July 10, 2014, 7:17 p.m.

    ...company won’t go anywhere. The team should initially consist of 2-3 individuals that are aligned in vision and...

36. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...programs. The groups in these programs typically consist of 1-2 mentors and 3-5 mentees who meet together...

37. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...the level of gene expression. For example, the synthesis of deoxychromoviridans requires three genes whose...

38. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...protein-of-interest, increasing target selectivity, overcoming resistance to inhibitors, and inducing prolonged biological...

39. RANbodies: Reporter Nanobody Fusions

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    Blog Post

    Published

    April 10, 2018, 12:56 p.m.

    ...secondary antibody. Chicken IgY This RANbody reporter consists of two chicken IgY domains. Chicken IgY domains...

40. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    Published

    June 15, 2017, 2:30 p.m.

    ...Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...