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  1. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...clear plans to connect Genotype to Phenotype thus making progress on the NSF “G to P grand challenge.” Creating...like people. This is actually rare in the animal kingdom. Gasterosteus aculeates (Dan Bolnick) A paper on...

  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

  3. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...of Replication The Promoter Region – Let's Go! Looking for More Plasmids 101 Articles?  Find them all ...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a given wavelength...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... so, the WHO recommends one strategy: testing, tracking, and social distancing. With countries like South...relies on its activity on the target regions thus making it an affinity-based method. Due to the mismatch...

  8. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...fed a diet of primarily egg whites would develop skin lesions and other terrible health issues. This disease...PMC3223083. 6. McInnes, J. L., Forster, A. C., Skingle, D. C., & Symons, R. H. “Preparation and uses of...

  9. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, adds: "If you have... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ... signal is boosted and enables single molecule tracking in living cells. The HA frankenbody is an HA tag...also be fused to cellular toxins, radioisotopes, cytokines, and enzymes for cancer, autoimmune, and/or inflammatory...

  11. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...optimal promoter for your gene of interest, painstakingly cloned into the perfect empty backbone, made...Promoter Region – Let's Go! Mammalian Vectors Looking for More Plasmids 101 Articles?  Find them all ...

  12. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ... MQ1.) The Q147L mutation improves methylation kinetics such that cytosine methylation occurs within 24...Sander JD, Reyon D, Bernstein BE, Costello JF, Wilkinson MF, Joung JK (2013) Targeted DNA demethylation...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...cell types and are useful if you're going to be working in several different systems or want to transduce...j.omtn.2017.03.009 Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaji, M., Isa, K., Mizukami, H., Ozawa,...

  15. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...been observed to occur in many different kinds of plasmids, taking many forms (Levene, 2009; Higgins & Vologodskii...

  16. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...gRNAs to choose from. With so many possibilities, picking a gRNA with an optimized sequence is of primary...detectable mutations (Veres et al., 2014). When working with single-cell clones, the authors note that ...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...mutations are essential data points for investigators looking to definitively link genotype and phenotype. It... and do not require high sequencing read counts making them fast and viable options for multiplex sequencing...

  18. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...false negative or conclude the viral vector is not working.  When using a “cell-type specific” promoter, such...

  19. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...photographer at the Super Bowl, a medical technologist taking an x-ray, or a biologist imaging the smallest structures...microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic or static...

  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...cytometry particularly well to complex experiments looking at a variety of different targets. If you are planning...43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry: An Overview. Current...

  21. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Base editors chemically modify DNA bases without breaking the DNA backbone, sidestepping the problem of ... 98-99% target modification in primary T cells, making them a promising tool for cell therapy applications...

  22. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...efficiency is so high that some applications may permit working with edited “pools” of cells, eliminating the time-consuming...telomere length. Telomere trimming can be assayed by looking for the production of ECT-DNA (extra-chromosomal...

  23. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...Looking for a fast and inexpensive way to determine your antibody's purity? Check out our latest protocol...

  24. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...

  25. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...for Protein Innovation (IPI). Together, we’ll be working to provide researchers with a collection of IPI...

  26. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...If you’re looking for the best way to improve your lab techniques — but perhaps are a little limited ...

  28. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

  29. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...recently chosen four names as finalists. Now, we’re asking you to help us make the decision between some meaningful...

  32. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...

  33. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...

  34. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... has a large international audience, including working with distributors across the world to help increase...

  36. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...keep track of reagents in your lab. With barcode tracking, you will always be able to locate materials quickly...

  41. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...all ourselves. Therefore, in this blog post we’re taking a moment to reach out to you, our readers, and ...

  42. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...powered by 100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown...

  45. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...download the CRISPR eBook today! P.S - If you’re looking for a quick refresher before you dive into the ...

  46. New Viral Vectors - Spring 2025

    Type
    Blog Post
    Published
    April 29, 2025, 1:30 p.m.
    ...AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? Browse our in-stock viral...

  47. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    ...your viral packaging service in the same place, making it easier to go from selection to viral vector....

  48. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...Feng New viral service, multiple serotypes Looking for more viral vectors? Browse the full viral services...

  50. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Officer. Prior to Addgene, Kenneth was an investment banking analyst at Credit Suisse’s Technology Group in ...
Showing: 601 - 650 of 778 results