We narrowed to 276 results for: ATC

1. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...the denticles actually clump together forming one patch on the surface, giving the embryo a “spiny and prickly...

2. Lab to Office Culture Shock

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   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ...Resources at Addgene.org Find careers at Addgene Watch out career videos Sign up for the Addgene quarterly...

3. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...Find tips on choosing an electronic lab notebook Watch a livestream of the Minisymposium on Reproducibility...

4. CRISPR-mediated Plant Base Editors

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   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...resultant U-G pair is either repaired by cellular mismatch repair machinery making an original C-G pair converted...

5. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ... on Addgene.org Find Plasmid Cloning Protocols Watch Our Diagnostic Restriction Digest Video Check Out...

6. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ... dire prognosis for mesothelioma patients is to catch the disease earlier. New techniques are currently...

7. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence. The dsRNA can be delivered...

8. Hot Plasmids: Summer 2024

   Type

   Blog Post

   Published

   Aug. 27, 2024, 1:15 p.m.

   ...beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins ending with a GFP...

9. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...cleavage at predicted off-target sites with up to 7 mismatches to the gRNA target sequence. Kaminski et al. ...

10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ... However, since some guide RNAs may tolerate a mismatch in the binding sequence, we recommend changing...

11. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...

12. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...Where do they come from and what do they mean? A Match Made in Heaven: CRISPR and AAV Resources on Addgene.org...

13. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...development of many distinct CRISPR therapies to match these different mutations. For each therapy, effectiveness...

14. Plasmids 101: Common Lab E. coli Strains

    Type

    Blog Post

    Published

    Nov. 7, 2014, 2:56 p.m.

    ...Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying...

15. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 2:15 p.m.

    ...have created many engineered serotypes by mixing, matching, and modifying capsid and genomic structures. ...

16. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ...fluorescent secondaries on hand in the lab to mix and match with primaries for whichever targets you like. Plus...

17. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...incorporating low metabolic burden seed-stock and fed-batch fermentation processes. Biotechnol Bioeng. 2009 ...

18. Antibodies 101: The Basics of Western Blotting

    Type

    Blog Post

    Published

    March 8, 2021, 2:41 p.m.

    ...tissues to demonstrate that the protein staining matches the expected expression patterns across tissues...

19. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ...Addgene.org Find Vectors with the piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit...

20. How to Design Your gRNA for CRISPR Genome Editing

    Type

    Blog Post

    Published

    Sept. 24, 2020, 1:15 p.m.

    ...important to consider. While the basic landscape of mismatches that can nevertheless still lead to activity ...