We narrowed to 337 results for: CAPS;

1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ...synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative...

2. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used under the Creative...

3. Antibodies 101: Stripping and Reprobing Western Blots

   Type

   Blog Post

   Published

   Aug. 1, 2024, 1:15 p.m.

   ...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

4. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...has been used to assemble multigene constructs capable of performing production and subsequent necessary...

5. CRISPR in the Clinic

   Type

   Blog Post

   Published

   Nov. 2, 2023, 1:15 p.m.

   ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

6. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References...

7. Viral Vectors 101: Chemogenetics

   Type

   Blog Post

   Published

   July 11, 2023, 1:15 p.m.

   ...expressed in the brain, it is necessary to use ligands capable of crossing the blood-brain barrier.  Another consideration...

8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 3, 2022, 1:15 p.m.

   ...Plasmids) PI Blue EBFP2 EBFP2(1–10) and Capri(1–10) for use with GFP(11) Multiplexed labeling ...

9. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...Sequencing (CPS) workflow and yield sufficient data to capture the entire viral genome. The full details of that...

10. Tips for Technical Support Calls

    Type

    Blog Post

    Published

    Sept. 20, 2016, 2:39 p.m.

    ... science, but we don’t have the resources to recapitulate the experiments performed by our thousands of...

11. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...technologies that has transformed the assay’s capabilities. Specifically this includes primer directed ...

12. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ... work with than mammalian cells, and are often capable of modifying complex proteins unlike bacterial ...

13. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type

    Blog Post

    Published

    March 28, 2018, 6:52 p.m.

    ...residues D10 and H840. To ensure that the clones were capable of cleavage, they tested the clones against an ...

14. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ..., find out what their learning styles are and capitalize on them for the benefit of the team. By properly...

15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the biotinylated target...

16. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right...

17. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...through DNA degradation, thanks to the unique capabilities of Cas3 and its partner in crime, Cascade. Find...

18. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ... of Cas9 – primed for cleavage. This structure captures the displaced non-target DNA strand and shows ...

19. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ...with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses them in our own AAV ...

20. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo...