We narrowed to 334 results for: CAPS;
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TypeBlog PostPublishedAug. 10, 2023, 1 p.m....studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References...
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Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....expressed in the brain, it is necessary to use ligands capable of crossing the blood-brain barrier. Another consideration... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021. Alternative... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used under the Creative... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m....has been used to assemble multigene constructs capable of performing production and subsequent necessary... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....Sequencing (CPS) workflow and yield sufficient data to capture the entire viral genome. The full details of that... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m.... science, but we don’t have the resources to recapitulate the experiments performed by our thousands of... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog PostPublishedAug. 30, 2016, 2:30 p.m....technologies that has transformed the assay’s capabilities. Specifically this includes primer directed ... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m....academia, however, writing papers and grants is an inescapable and essential part of being able to continue ... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m.... work with than mammalian cells, and are often capable of modifying complex proteins unlike bacterial ... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....residues D10 and H840. To ensure that the clones were capable of cleavage, they tested the clones against an ... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m...., find out what their learning styles are and capitalize on them for the benefit of the team. By properly... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the biotinylated target... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....through DNA degradation, thanks to the unique capabilities of Cas3 and its partner in crime, Cascade. Find... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....undifferentiated “stem” state and, Their potency or capability to differentiate into specialized cell types ... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m.... of Cas9 – primed for cleavage. This structure captures the displaced non-target DNA strand and shows ...