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We narrowed to 622 results for: Dos

Showing: 241 - 260 of 622 results

  1. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...in our lab’s environmental impact, but we can all do more to make research more environmentally friendly...recycling program at your institute! What should I do in the lab to help the environment? Well, you could...

  3. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...are not enough to move the CRISPR field forward. To do that, we need to learn much more about cellular DNA... just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR Genome Editing...

  4. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...call out every Addgene Team because they’re all doing amazing work during this difficult time. One function...distributes and fulfills requests for scientists doing almost every type of biomedical science research...

  5. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...So you have this awesome experiment you want to do, but it requires some AAV. You’ve never worked with... but you aren’t going to let that stop you. Where do you start? Turns out like all good experiments, making...

  6. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...plasmid to manipulate and use in later work.     How do scientists use plasmids? Generally, scientists use...Some of the many things that plasmids can be used to do include: Produce large amounts of a protein so that...

  7. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...at a peer audience.  Note that lay people can and do consume scientific communication (it is, after all...moves from layperson to expert) and peers can and do consume science communication.  Formal education ...

  8. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...100percentSDS.     Additional Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript ...introduction, etc. so as to thoroughly explain the do’s and don’ts of that week’s piece of a manuscript....

  11. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...chemically coloured lymphoid organs did in the past. How do researchers track fluorescently labeled cells? 1.... answer using two-photon microscopy include: How do T cells and/or Neutrophils travel to the sites of...Chtanova et al. 2008, Kamenyeva et al. 2015)? How do blood monocytes patrol blood vessels during immune...Auffray et al. 2007, Finsterbusch et al. 2016)? How do dendritic cells migrate to lymph nodes to activate...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ... that is right for you. What type of plasmid prep do you need? As we covered in a previous post on plasmid...midiprep, etc. (more on prep sizes below). But what do we mean by DNA quality? There are a few different...starting culture conditions rather than your kit.   Do you want eco-friendly options? It’s no secret that...  While budget is an important consideration, you do want to make sure you’re getting the best bang for...

  13. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ...guides as negative controls. Non-targeting guides do not target any site in the genome. This means that...confound the results of high-throughput CRISPR screens. Do short guides (17-18 bp) have less off-target cutting...have fewer off-target effects than long guides, and do not have a major reduction in on-target efficacy....

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...train of action potentials that GCaMP6 and jGCaMP7 do not. Most likely, it’s a combination of many factors...supporting data and community engagement required to do this drove Addgene to develop new systems, new partnerships...resources, and collaborations, and will continue to do so, in the hopes that these findings can help others...

  15. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...site-directed knock-in. We’ll also discuss what to do if your editing efficiency isn’t as great as you ...insertion or deletion that isn’t a multiple of three will do. A popular screening method for knockouts is Tracking...base pairs modified? In these cases, it’s best to do some planning in the design phase of your experiment...

  16. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... resting phase of the cell cycle; quiescent cells do not take up nucleic acid or express transgenes as...economical mode of detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely...Learn All About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...expression levels for most purposes, but what do you do when you’ve tried a common strain and don’t get...popular commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from...

  20. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...physics and sustainable design while allowing me to do my internship in the daytime.  Getting into the office...be heavy restrictions on what I could and couldn’t do as an intern. I expected that these restrictions ...
Showing: 241 - 260 of 622 results