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Showing: 241 - 260 of 371 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...Shakked O., et al. "CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...a virus can infect. The VSV-G envelope protein enables this virus to infect many different cell types ...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...isotype control for that particular marker. This enables you to identify any non-specific binding to know...

  5. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...sharing speeds science, and our entire mission is to enable that process. Our repository of plasmids, AAVs,...

  7. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...considering the need for some manner of polyA. In this scenario, you may be best served by a dual vector, split...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...native proteins (so antibodies validated only for denatured proteins may not be a good choice) and the amount...

  9. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by identifying...

  12. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ...journal.pgen.1004086 Ghezraoui H, Piganeau M, Renouf B, Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson...

  13. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... mainly on the elements required for plasmid maintenance within an E. coli cell, but vectors can be widely...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...segment is labeled with fluorescent probes that enable visualization of the location of the protein-protein...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...process a large number of microbiome samples. To enable large-scale, longitudinal studies of the gut microbiome...

  20. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...change. If your protein might become slightly denatured during your experimental protocol, using a polyclonal...
Showing: 241 - 260 of 371 results