We narrowed to 559 results for: ESP

...you can inhibit cell cycle regulatory proteins responsible for the transition out of S phase, so cells stay...Methods favoring homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int...

... figure organization with a different person responsible for each figure. Others still are more casual...focus and critical mass of participants. This is especially true of journal clubs where the very point is...

...AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern similar...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

...also very important in a non-research context, especially when considering small to mid-sized companies...When you reach out as a student, people tend to respond in a supportive way and are willing to help because...

...which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic...small molecules that do not generate an immune response. Aptamers can be selected for these small ligands...

...mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, the greater...cell, and binds cells that can begin an immune response. This important biomarker can be used to distinguish...

...which, for example, the activity of all genes in response to an experimental treatment can be monitored....CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...

...pathways generating interesting drug-like compounds especially cardenolides which have been used to treat heart... and one is promiscuous and the gene that is responsible is understood. It’s possible to actually silence...

...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities...

...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...

...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...

...shipping. However, there were issues that persisted despite our best efforts. With a little over half of our...time when a picture is worth a thousand words, especially when it comes to protocols. In 2013, we launched...

...counterparts. Those familiar with viral vectors, and especially those familiar with adeno-associated viruses ...chemogenetic tools: protein tools that are activated, respectively, by substrates, light, or engineered ligands...

...procedure is longer than alternative ELISA methods, especially when secondary antibodies are used.  Competitive...positives.  If testing a recombinant protein, especially one with tags, be sure to include a sample that...

... editing (no transgene integration), which is especially valuable for regulatory approval and public acceptance...tools. Its small size and unique features make it especially promising for delivery via viral vectors and ...

...Converts A to Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, ..., or C base editors Excises G, T, or C, respectively, to be replaced with another base. References ...

...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...

...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can...

...but like to be able to provide well thought out responses. I hung out with Joanne as she answered questions...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was...