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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 554 results for: ESP

Showing: 241 - 260 of 554 results

  1. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...AsCpf1 and LbCpf1 (1307 and 1228 amino acids long respectively), both cleave in a staggered pattern similar...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

  3. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...Converts A to Y (T or C), X (any base), or C, respectively. gGBE, gTBE, gCBE Glycosylase-based G, T, ..., or C base editors Excises G, T, or C, respectively, to be replaced with another base. References ...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...shipping. However, there were issues that persisted despite our best efforts. With a little over half of our...time when a picture is worth a thousand words, especially when it comes to protocols. In 2013, we launched...

  5. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ...counterparts. Those familiar with viral vectors, and especially those familiar with adeno-associated viruses ...chemogenetic tools: protein tools that are activated, respectively, by substrates, light, or engineered ligands...

  6. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...procedure is longer than alternative ELISA methods, especially when secondary antibodies are used.  Competitive...positives.  If testing a recombinant protein, especially one with tags, be sure to include a sample that...

  7. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...also very important in a non-research context, especially when considering small to mid-sized companies...When you reach out as a student, people tend to respond in a supportive way and are willing to help because...

  10. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...supports reproducibility RSpace was designed in response to requirements that emerged from intensive engagement...developed with these principles in mind, and in response to further input provided by the research communities...

  11. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...which protein fluors are likely to trigger immunoresponses. Nucleotides, in general, are far less immunogenic...small molecules that do not generate an immune response. Aptamers can be selected for these small ligands...

  12. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...envelope protein on the virus’s surface and a corresponding receptor in the host cell. Some of these envelope-receptor...infecting neurons and cause minimal inflammatory response, but are not taken up by axon terminals and can...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...experimental target, SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our ... using a Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ...neighboring ssRNA and ssDNA by Cas13 and Cas12a, respectively, to cleave and activate a reporter. The detectable...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert...

  15. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Gern, J., & Seroogy, C. (2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell...Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant Materials. PloS One, 10, e0137550...

  16. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...reported or posted on the material page for the corresponding catalog item (see maps section for images). ...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

  17. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...mesothelioma many years later. While there is a dose-response relationship (the higher the exposure, the greater...cell, and binds cells that can begin an immune response. This important biomarker can be used to distinguish...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...but like to be able to provide well thought out responses. I hung out with Joanne as she answered questions...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was...

  19. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...are many reasons why client situations might be especially challenging. For instance, the client might need...at business schools, and consulting clubs are especially helpful since they both provide up-to-date information...

  20. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    Published
    Oct. 1, 2015, 2:30 p.m.
    ...determine this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation...cue. The hippocampus has long been known to be responsible for mediating this behavioral effect but the ...
Showing: 241 - 260 of 554 results