We narrowed to 570 results for: FIE

... For an estimate on availability date or to be notified when a particular catalog item is available for... the vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name ...

...molecular biology teamwork. The guide RNA (gRNA) specifies the editing target site on the DNA, the Cas domain...enzyme. After testing numerous TadA variants, they identified TadA-8e, which edits 590-fold faster than TadA...

...constitutively active CMV promoter. Constructs were then verified by both diagnostic restriction digest and colony...generated mAb and the R-mAb, with the R-mAb being verified when the labeling matched that which was observed...

...optimized genetic screening technique, researchers identified a synthetic lethal interaction between MARCH5... available separately. The proteins, which are purified via histidine tags, are optimized for SDS-PAGE...

...hamsters, although no cancer-connection has been identified in humans to date. Over fifty serotypes of human-infecting...human-infecting adenovirus have been identified so far! Adenovirus generally has high immunogenicity, ...

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...SLIC cloning process, a gene of interest is PCR amplified to add 5′ and 3′ homology regions from the target...assembly with SLIC. Multiple fragments are PCR amplified to add 5′ and 3′ homology regions to the adjacent...

...understand brain function So far scientists have identified 180 brain regions. Both this geography and the...all by this serotype. Nichole Beebe in Brett Schofield’s lab demonstrated that pathways projecting to ...

...biology. In our case, we selected a project in a field with 30 years of basic research standing behind ...are well understood. Additional work has also identified and characterized mechanisms and components of...

...During this project, authors are asked to add an identifier (a bit like a GenBank ID) to all antibodies, ...a paper is received by other researchers in the field. Dos and don’ts when publishing a scientific manuscript...

...protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene: What...Musunuru: The CRISPR/Cas9 system is easier to use as testified by the fact that the TALEN kit includes 834 plasmids...

...diversity in your gRNA library, but this should be verified by NGS before beginning your experiments. .... Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-virus-induced cell...

...processes. Every plasmid we receive is rigorously verified before becoming available to the community. This...overnight cultures with 15% glycerol), as opposed to purified DNA or colony sequencing. This means that we are...

...correct insert Sequence the regions that have been amplified by PCR, putting special attention in the areas...try a different cloning strategy. Once you've identified potential clones after the screening, sequence...

...variant characteristics they wanted, the team identified Archon1, a novel opsin-based fluorescent voltage...because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid tools were also...

...Mitalipov and his colleagues have achieved, and how the field will move forward from this work. Experiments & ...paper represents a large step forward in the CRISPR field, it’s important to remember that the study hasn’...

...part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent probes...protein levels in situ, the Schumann lab used a modified version of PLA where one antibody was directed...

...there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder since ...Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert...

...how much faster it would be for an expert in the field to read this paper than it would be for an undergrad...the length of your average PhD to achieve!) Each field has its own framework: a particular way of organizing...

...discern the proteins in your assay.  Narrowing the field After working your way through the above steps, ...publication. Talk with your peers. If someone in your field has used the antibody before you will not only have...