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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 566 results for: FIE

Showing: 241 - 260 of 566 results

  2. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ... aren’t the only investigators in the chromatin field who have deposited with Addgene, but you’ll find...DW Cell. 2017 Aug 24;170(5):956-972.e23 Screen identifies bromodomain protein ZMYND8 in chromatin recognition...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple targets compared ...s ability to process its own pre-crRNA arrays simplifies the crRNA cloning process. For cloning, Zetsche...

  6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...high number of background colonies carrying the unmodified plasmid. For insertions, it’s possible to use...No No Yes Yes DpnI Digestion Yes Yes No No Purified Enzymes Required No No Yes Yes Lab Made Competent...

  7. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...biology. In our case, we selected a project in a field with 30 years of basic research standing behind ...are well understood. Additional work has also identified and characterized mechanisms and components of...

  8. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...During this project, authors are asked to add an identifier (a bit like a GenBank ID) to all antibodies, ...a paper is received by other researchers in the field. Dos and don’ts when publishing a scientific manuscript...

  9. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...protocol, it is feasible to produce genetically modified cells in less than a month's time. Addgene: What...Musunuru: The CRISPR/Cas9 system is easier to use as testified by the fact that the TALEN kit includes 834 plasmids...

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...the lack of an Fc region. The lack of Fc also simplifies analyses when used in structural studies and ...1986) Two embryonic, tissue-specific molecules identified by a double-label immunofluorescence technique...

  11. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...how much faster it would be for an expert in the field to read this paper than it would be for an undergrad...the length of your average PhD to achieve!) Each field has its own framework: a particular way of organizing...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...discern the proteins in your assay.  Narrowing the field After working your way through the above steps, ...publication. Talk with your peers. If someone in your field has used the antibody before you will not only have...

  13. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ... While secondary antibodies are useful signal amplifiers, the flexibility of their use varies across applications...each protein of interest, this gel-based method identifies proteins solely by weight and size, relying on...

  14. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder since ...Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...part in rolling circle DNA amplification. The amplified DNA segment is labeled with fluorescent probes...protein levels in situ, the Schumann lab used a modified version of PLA where one antibody was directed...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...Mitalipov and his colleagues have achieved, and how the field will move forward from this work. Experiments & ...paper represents a large step forward in the CRISPR field, it’s important to remember that the study hasn’...

  18. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...correct insert Sequence the regions that have been amplified by PCR, putting special attention in the areas...try a different cloning strategy. Once you've identified potential clones after the screening, sequence...

  19. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...diversity in your gRNA library, but this should be verified by NGS before beginning your experiments. .... Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-virus-induced cell...

  20. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...processes. Every plasmid we receive is rigorously verified before becoming available to the community. This...overnight cultures with 15% glycerol), as opposed to purified DNA or colony sequencing. This means that we are...
Showing: 241 - 260 of 566 results