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Showing: 241 - 260 of 309 results

  1. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

  9. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow...

  12. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

  13. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  14. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    Published
    Oct. 17, 2017, 12:55 p.m.
    ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

  19. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...depositor has indicated that there is a particular mutation found within the gene/insert, this information...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...
Showing: 241 - 260 of 309 results