We narrowed to 316 results for: Mut

1. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...inflammatory response. However, if dsRNA sensors are mutated or mechanisms which suppress endogenous dsRNA formation...

2. CRISPR 101: Cas9 vs. The Other Cas(s)

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   Blog Post

   Published

   Dec. 8, 2022, 2:15 p.m.

   ...engineering goals expand beyond generating frameshift mutations or single base pair edits, these enzymes are worth...

3. Antibodies 101: Affinity Reagents

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   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...fragments. However, rigidity can be introduced through mutation, allowing diabodies to be used for assembling ...

4. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   Published

   Sept. 22, 2015, 2:30 p.m.

   ...lung cancer cell line containing an activating mutation in epidermal growth factor receptor (EGFR) with...

5. Plasmids 101: Golden Gate Cloning

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   Blog Post

   Published

   Aug. 27, 2015, 2:30 p.m.

   ...amplification can be used to create silent point mutations at internal recognition site(s) thus eliminating...

6. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...potentially recombine. To account for this, alternate mutant versions of the loxP site have been created, which...

7. Overcoming the Challenges of Lentiviral Production

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   Blog Post

   Published

   Aug. 7, 2018, 12:31 p.m.

   ...promoter of the 5’ long terminal repeat (LTR) is mutated in third generation systems such that it no longer...

8. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...overexpression, knockdown (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together...

9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

10. Plasmids for Endogenous Gene Tagging in Human Cells

    Type

    Blog Post

    Published

    April 6, 2017, 1:02 p.m.

    ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

11. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

12. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

13. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...titration consider using a reverse transcriptase mutant in a separate control reaction. This will allow...

14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...seeing tRNAs because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed...

15. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    Published

    Sept. 17, 2015, 2:30 p.m.

    ...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

16. 3D Printing Meets CRISPR Cas9

    Type

    Blog Post

    Published

    Oct. 17, 2017, 12:55 p.m.

    ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

17. Truncated gRNAs for Regulating Gene Expression

    Type

    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability...

18. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

19. Degrading DNA with Cascade-Cas3

    Type

    Blog Post

    Published

    Feb. 11, 2025, 2:15 p.m.

    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

20. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...