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We narrowed to 730 results for: NSI

Showing: 241 - 260 of 730 results

  1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...method into six general steps and outlined the considerations to be made during each step. Now that you know...method of fixation is good for epitopes that are sensitive to cross-linking, but it doesn’t preserve sample...binding to any target protein that is still on the inside of the cell. However, assuming you do need to permeabilize... then optimizing from there is a good option.  Consider your target - if you are studying a membrane protein...

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...specific genomic region using the CRISPR system, consisting of dCas9 and targeting gRNA, to identify molecular...isolation of a specific genomic region was an intensive process. One method required insertion of a recognition...vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and synthetic ...high-affinity purification. This system is both highly sensitive and specific and allowed the Xu lab to identify...

  3. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...abandoned early on due to its considerable immunogenicity and its transient expression. LV results in long...been shown to to transduce the retina but only transiently. However new viral vectors capable of providing...disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe, childhood-onset...

  4. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...data with a 7-day rolling mean gave us a crude insight into the magnitude of the effect COVID-19 had on...holiday effects. Based on these observations, we considered the following (noiseless) model for our data:...factor 𝜙z if t is during event z. The "events" we considered for our model were weekends, Christmas/New Years...Europe. To improve the stability of our model, we considered only the 10 papers with more than 10 unique order...

  5. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...translation. Its genome consists of “early” (E) and “late” genes, and the transition between early and late...length. Human adenovirus type 5 (hAdV-C5) can be considered the prototype, although many viruses infecting...the majority of adenoviral vector expression is transient due to the fact that AdV surface proteins (especially...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ... in transit and some have been transported across oceans and continents. Each deposit may consist of anywhere...your lab ensure that sample ID’s are unique, consistently labeled, and that numbers are not switched around...Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids in our ...

  7. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    Published
    Aug. 20, 2015, 2:30 p.m.
    ...research grants, especially in the sciences and by extension to scientifically oriented nonprofits. Like Deb...career path changed when I was asked if I would consider consulting for a particular grant by another state...entering the Grants Profession. Whether you’re transitioning from a previous career or you’re starting your...above and formalizes your development. You may consider earning a degree in Grant Writing, Management ...

  8. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...these challenges, researchers have amassed a considerable molecular tool box of tags and fusion proteins...deploy proteosomes to degrade what the cell might consider junk protein. Though there are a number of chemical...EINAPTDGKV EKVLVKERDA VQGGQGLIKI GVHHHHHH  9 Combo Consists of a bacterially derived in-vivo biotinylation...and Gly residues   This article is not a comprehensive guide to all tags, but rather a quick overview...

  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...included tests done in cell lines known to be more sensitive to toxicity from RNA editing, such as mouse embryonic...CRISPR-driven precision into a genome, can be considered the fourth generation of CRISPR, one which drops...drops itself neatly into that gap. Consider, for instance, a disease like cystic fibrosis, which can be...that this system wasn’t dependent on the cell’s intrinsic machinery being already activated to work. (It...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...within Cas13a are essential for RNA cleavage, consistent with known roles for HEPN domains in other proteins...located at the 3’ end of the spacer sequence and consists of a single A, U, or C base pair. In bacteria,...bacterial cells will likely be limited by the propensity of Cas13a to bind and cleave RNA non-specifically...Finally (and perhaps most interesting), Cas13a’s propensity to cleave any RNAs after binding a user-defined...

  11. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...that evolved in the last 10,000 years (which is considered recent). They have diverse traits with good genetic... and one is promiscuous and the gene that is responsible is understood. It’s possible to actually silence... to get a recombinant because this process is expensive. They are using a cool fluorescent indicator system...trying to replace embryo injection (hard to do, expensive, not every lab has the capability) with receptor...

  12. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...The ACE reporter is a bicistronic construct that consists of a mutated mCherry and a downstream constitutively...that results in the restoration of fluorescence. Transient reporter for editing enrichment (TREE) The Wang...Wang and Brafman lab developed a transient reporter for editing enrichment (TREE) to purify single base...Schwarz G, Nguyen T, Wang X, Brafman DA (2019) A transient reporter for editing enrichment (TREE) in human...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...show a high density of cells, while contour lines that are spread apart show a low density of cells (Figure... a ‘gate’; it tells the machine that everything inside the gate is one population and everything outside...Heat maps are often used to indicate the relative density of cells. You can see both of these features in...

  14. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...vector into your target cells; you also have to consider how to keep it there. In fast-growing populations...needed to retain the vector as cells divide. In transient transfection, the vector remains episomal, meaning...about vector replication at all. In these cases, transient transfection may be sufficient for your needs ...involved in the production of viral vectors, we consider the viral vectors themselves to be a distinct ...

  15. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...letters, while higher-level jobs are more likely to consider cover letters an important part of the application... content team, where writing is a core skill, considers cover letters to be very important. On the other...unofficial poll, hiring managers also said that they considered cover letters to be more important for higher-level...communication skills” try giving an example, like “I have extensive communication experience, having given more than...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...systems that are widely present in bacteria. They consist of a toxin which can affect a variety of cellular...division”). The encoded toxin-antitoxin system consists of CcdB (toxin) and CcdA (antitoxin) where CcdB...systems Because restriction modification systems consist of two enzymes that have opposing activities, it...the systems just described, the hok/sok systems consist of three components: Host killing (hok), a long-living...

  17. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...Previously, we have described a number of practical considerations that should be taken into account when deciding...needs. Equally important, before cloning you must consider whether and how well your FP will function in ...fluorescent proteins in your experiments, please consider the following: How can one ensure that an FP ...compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the reasons...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...using microscopy techniques. FRET measurement sensitivity makes it suitable for studying interactions within...are difficult to overcome but they have to be considered when analysing data from your FRET experiments... The simplest and the most popular one is the sensitised emission method, where the donor is excited by...acceptor fluorescence, this method is also less sensitive to direct acceptor excitation artifacts and it...

  19. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two components...scFv-GFP fusion bind the SunTag scaffold, the intensity of the fluorescent signal is boosted and enables...to the disulfide bond independent folding of intrinsically stable scFv. PLoS ONE 12:e0189964 . https://...which uses an scFv to amplify the fluorescence intensity of a tagged protein. Resources on Addgene.org...

  20. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...have, and we developed a great plan to start consistently knocking out high-quality videos for you all...two options: Film outside at a distance, or film inside with one person in a room. I wouldn’t be in there...live. After doing a bit of looking, I found an inexpensive HDMI transmitter that could transmit a signal... stand and mounted a monitor to it. It had an extension cord attached, with the option to mount a battery...
Showing: 241 - 260 of 730 results