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TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....expressing cells exhibited a 2-4 fold increase in the number of endogenous indel edits when compared to unsorted...
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Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog PostPublishedJuly 28, 2017, 1:30 p.m....overexpression and knockdown. Addgene also has a number of insect vectors to consider. An easy way to see... -
Improved Plasmid Maps Powered by SnapGene
TypeBlog PostPublishedJune 29, 2017, 1:06 p.m.... enzymes will cut (in basepairs), and the total number of times they cut. Use the filters on the right... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....high luminescent signal for imaging of low-copy number proteins, whereas such detection is difficult using... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....variable expression levels due to differences in copy number. And there are the usual transfection drawbacks... -
Hot Plasmids - May 2022
TypeBlog PostPublishedMay 26, 2022, 1:15 p.m....due to multiple factors, including limitations of number of flourescent proteins that can be inserted into... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog PostPublishedDec. 15, 2017, 2:07 p.m....neuroscience and, like many biological fields the number potential of experiments enabled by physical, software... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....specifically incorporated ncAA, ready for you do to any number of experiments with. The catch The rapid expansion... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....statistical modelling can be used to determine the actual number of target DNA molecules present in the original... -
Addgene’s Viral Service - Why Virus? Why Now?
TypeBlog PostPublishedMarch 23, 2017, 2:30 p.m....we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m.... found the most useful so far? We crunched the numbers on our Viral Service (and then we crunched them... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....hybridoma impacts the specificity, avidity, and number of different antibodies produced by the hybridoma... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited and asked for the protocol... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....considering the gRNA itself is ~100 bases) limit the number of gRNAs that can be packaged together in a single... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m.... said that there is no theoretical limit on the number of genes that can be manipulated at one time. Weissman... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m.... we going to talk about?” There are an infinite number of ideas on the internet, but start by downloading... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG-13 in... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....neutral vesicles to more acidic lysosomes. Though a number of biosensors existed that met some of the criteria... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....1,000 ready-to-use viral vector preps. Today, that number has grown to over 114,600 viral preps. And in 2025... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of ...