Skip to main content
Addgene
Showing: 241 - 260 of 537 results

  2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...aggregates. This property can be tolerated in some applications (e.g. labeling of cells or tracking promotor...promotor activity), but it is problematic in applications in which the fluorescent protein is used as an inert...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...reached at high power may not translate to real applications in which far less power is used. For example...insight into the photostability in the intended application (Goedhart et al, 2012). The third issue relates...

  4. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds like a nifty technique...help answer? Ramanathan et al. outline three applications of RaPID: Discovery. To discover novel RNA-...

  5. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

  6. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...experiments. Recombinant AAVs (rAAVs) The primary application of AAV is delivery of genetic cargo to a host... S., et al. “Various AAV Serotypes and Their Applications in Gene Therapy: An Overview.” Cells, 12(5),...

  7. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... out large regions of DNA and has anti-viral application potential as a result. Fast facts – PAM requirement... has been proposed to be useful for the same applications (RNA knockdown, RNA editing, etc.). The main...

  8. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, Twitter...listing “Submitted” articles in their CV or grant applications. A number of organizations (such as ASAPbio)...

  9. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...gRNA binding a non-mammalian sequence (GAL4) was supplied. In addition to labeling repetitive telomeric...single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure 2: CRISPRainbow...

  10. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ... is almost always worth it. Because labels are applied to containers and not the mushy, gooey contents...be!) bear in mind that your barcodes are being applied to the containers, not to the contents. Tightly...

  12. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...were so many interesting questions that we could apply [it to].” Using ClonTracer to study drug resistance...Novartis Oncology and is particularly interested in applying what we have learned about tumor heterogeneity...

  13. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...engineering fields. We’ll walk you through how to apply this precise and easy-to-use system to your cloning...different combinations, or other new and exciting applications, this system is an incredibly powerful tool ...

  14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...it comes to targeting precision and practical application. If the CRISPR/Cas9 system is not effective for...lncRNAs with high specificity, particularly in applications requiring smaller delivery systems (Zibitt et...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... for lightning-mediated gene transfer in soil. Applied and Environmental Microbiology, 67(8), 3440–3444...Bacteria:Their Characteristics and Potential Applications in Biotechnology. Critical Reviews in Biotechnology...

  16. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have...

  17. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  19. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...and who will be affected by the knowledge and applications generated from this research. At both a practical...
Showing: 241 - 260 of 537 results