We narrowed to 289 results for: Pol
-
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...
-
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....endogenous CRBN E3 ligase complex which will polyubiquitinate the fusion protein. This will target the protein... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....the surface of the protein where the nascent polypeptide emerges from the exit channel. He then said... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....HaloTag, SpyTag). The Foerstemann lab developed a polymerase chain reaction (PCR)-based system that allows... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....Chemical transfection Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Praparaten. Leipzig: S. Hierzel. J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog PostPublishedNov. 29, 2022, 2:15 p.m....have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog PostPublishedSept. 8, 2016, 2:30 p.m.... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m....has four layers of location which can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m.... Marc Sze, Victoria McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list... -
Transferable Skills Guide: External Collaborations
TypeBlog PostPublishedApril 19, 2018, 1:25 p.m....skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog PostPublishedNov. 15, 2017, 3 p.m....Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary... -
Career Insights: Technical Support Specialist
TypeBlog PostPublishedNov. 9, 2017, 2 p.m....the job. When dealing with customers, you must be polite, friendly, patient, attentive, empathetic, and ... -
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?
TypeBlog PostPublishedOct. 13, 2022, 1:45 p.m....engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog PostPublishedSept. 17, 2019, 12:40 p.m.... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m...., including investigations into environmental pollutants and health, embryo growth, brain function, and...