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We narrowed to 610 results for: REV

Showing: 241 - 260 of 610 results

  1. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...x-ray machine not on teeth, but on blossoms, thus revealing the startling inner beauty of familiar flowers...such as those collected by Darwin on his voyage. Previous destructive techniques were incompatible with ...

  2. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

    Type
    Blog Post
    Published
    Dec. 17, 2013, 2:47 p.m.
    ...another is not just to share your riches but to reveal to him his own." -Benjamin Disraeli Mentor, Sponsor...and more. It is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is...

  3. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian ...multiplexed gRNAs in human pluripotent stem cells. Previously, CRISPRi or CRISPRa depended on continued expression...

  4. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...assembly from cryo-electron microscopy, the team reveal the mechanism behind its activity including an ...motif, averaging 19 targets per gene. The lab has previously used the library with SpdCas9 to perform pooled...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...As previous blogs have noted, plants are an important foundation for life on Earth. Selective breeding... resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...

  6. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...transcriptional activation/repression. dCas9 has also previously been adapted to fluorescently label a sequence...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...

  7. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat of partial rather...toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used successfully in Drosophila, so...

  8. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...to control their own future in science (see my previous blog on this).  Indeed, that was a primary message...rules and practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled...

  9. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...variety of careers outside of academia. She has had previous graduate students and postdocs go on to work in...what you are truly passionate about. Check out my previous post if you're looking for more information about...

  10. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...lentiviral infection into target cells that have previously been stably transfected for Cas9 expression. ... in bacterial toxin resistance, identifying 4 previously unknown genes.  “With this work, it is now possible...

  11. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...bacterial chromosome that stores a genetic record of previously encountered foreign nucleic acids. The unusual...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/...

  12. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...the Camelid family, I wanted to take a moment to revisit some of the ways these animals have impacted humans...Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int...

  13. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...genome, thus providing an avenue for temporary/reversible CRISPR modification. In addition to these subpages...

  15. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...organism, all cells of that type are affected. Previous tools to overcome this restraint include an AAV-based...into an attB site. In the SPARC system, PhiC31 irreversibly recombines one of the two competing attP sites...

  16. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...and the species they are compatible with are all reviewed here. What are affinity tags? Affinity tags are...chromatography requires proper folding of the tag, which prevents insoluble fusion proteins from being purified ...

  17. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... with inappropriate activin-ACVR1 interactions prevents FOP bone growth in mouse models. As this biologic...Resources on the Addgene Blog Learn about Addgene's Previous Work with Rare Disease Researchers Check Out Potentiall...

  18. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set...related versions that have different properties. To prevent misidentification, we augment the database with...

  20. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...CRISPR construct needs to be segregated away to prevent continued production of new alleles or off-target... generation when CRISPR needs to be removed to prevent further editing, then green offspring can be selected...
Showing: 241 - 260 of 610 results