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Showing: 241 - 260 of 481 results

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The Foerstemann lab developed...Beyond simply monitoring protein localization and activity, CRISPR tagging systems can be modified to assay...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...bead assay (Figure 2). After finding no cross-reactivity, antibody candidates underwent affinity screening... that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible CRISPRi library...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute for HIV-1 Env,... particles with the VSV-G envelope have high infectivity in a wide range of cell types, so they’re ideal...

  6. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ... but in less time. The authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours... faster, cheaper, and had a similar level of sensitivity. Figure 2: Steps to purify DNA using a ...

  7. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...conformational changes in the antigen or lower detection sensitivity of the assay. Where possible, it can be useful...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....

  8. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...figures 4A-C). And since they have limited cross-reactivity between different IgG subclasses and different...State University Use Nanobodies to Control Protein Activity Learn more about protein tags Read about E. coli...

  9. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...more challenging to design a hands-on interactive activity using elephants than it is to design one demonstrating...or do you need a full exhibit? In a five minute activity at a street fair, or a semester-long after school...

  10. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...expressed as fusions that retain their individual activities (1). Researchers also discovered the modular ...activation (TA) domains retain their individual activities when separated (2), and that DBD and TAs from...

  11. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...it's fun to be involved in these professional activities because I can contribute by, for example, helping...

  12. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... situ. As a result, they had to be expressed in vitro before the bacteria were injected, limiting their... Use a subset of the cells to verify the mARG positivity, which can be observed under phase contrast microscopy...

  13. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...fusing mutants of Cas9 deficient in DNA cutting activity to a transcriptional activation domain (Fig 1)...Worry less about off targets Unlike Cas9 cutting activity, off target effects are generally not regarded...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ... Perhaps you, a colleague, or your adviser was invited to write on a particular topic. Alternatively, ...review articles Whether submitting a review by invitation or by your own accord,  once you have these rules...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ... Gateway cloning method, developed by Invitrogen, is an in vitro version of the integration and excision...

  19. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...the nucleus where they exert their recombinase activity (Logie and Stewart 1995; Metzger et al. 1995).... to compare neuronal morphology and synaptic connectivity before and after the gene knockout in the same...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ..., M., & Al-Qahtani, M. (2018). Evaluation of in vitro chondrocytic differentiation: A stem cell research...Minguela Puras, A., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T ...
Showing: 241 - 260 of 481 results