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TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m....Professor at Georgetown University, contributed to this post. Adeno-associated viruses (AAVs) make fantastic...enhance the efficiency of genome editing. In this post we'll walk through a variety of ways you can use...many applications of AAV in some of our other blog posts and have also been able to browse some of the ready... al. "CRISPR/Cas9-mediated genome editing via postnatal administration of AAV vector cures haemophilia...
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QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....stay up-to-date with these improvements. In this post, we discuss the sequencing technologies that Addgene...For more information on this process, see our blog post on NGS plasmid quality control. NGS is generally...process quickly while maintaining accuracy. This post was written by Alyssa Shepard, with significant ... -
CRISPR 101: Homology Directed Repair
TypeBlog PostPublishedJan. 26, 2023, 2:15 p.m....chemotherapeutics, and ultraviolet light. In this post, we will describe the mechanism of homology directed... than 10 bp away if possible. Check out our blog post on Improving HDR efficiency for more details on ...highest frequency of HDR edits. Check out our blog post on Easi-CRISPR for more details. References and...single-stranded DNA templates. Jacobi SA, et al. Poster at Keystone Precision Genome Engineering. 2017.... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....This post was contributed by guest blogger Derek Simon. The actual experiments you do will be determined...topic you’re interested in studying, but, in today’s post, we’ll discuss some of the important considerations...optogenetics to be fully summarized in a short blog post, but some examples I’m personally interested in ...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog PostPublishedSept. 1, 2015, 2:30 p.m....This post was contributed by Damien Wilpitz founder and lead consutant at Experiment Designs Consulting...start up budget, create a start up list. In this post we’ll help you successfully negotiate a start up... Additional Resources Find other career posts on the Addgene Blog! Learn more about Experimental... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m....This post was contributed by guest writer Pamela J. Hines PhD, Senior Editor at Science Magazine. Although...Out Our Career Resources Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog PostPublishedJuly 27, 2017, 1:11 p.m....their travel to a conference of interest. In this post, we’re proud to announce our first two Michael Davidson... of the ERK signaling pathway in our recent blog post. Fatima Enam: Biosensors for human milk oligosaccharides... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m....career you actually want, you can feel lost. Use this post to explore the wide range of careers available to...sending out applications. Check out our other career posts for more specifics on some careers and to find advice... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog PostPublishedApril 23, 2020, 1:15 p.m.... all of our viral vectors related blog posts Read our blog post on AAV titers Browse viral vectors protocols... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m....learn more about recombinant antibodies in this blog post. Hello, NABOR! Addgene will bring the same open-access... Addgene blog Check out our Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....This post was contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School...online guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining..., using a popular database management system (PostgreSQL), that aims to eventually be a comprehensive ... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog PostPublishedDec. 21, 2017, 2:06 p.m....This post was contribued by guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created...new product from the idea stage through all of its post-launch iterations. Additional Resources on the... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....This post was contributed by Deborah Sweet, Vice President of Editorial at Cell Press. Almost everyone...research more reproducible Read all of our blog posts on scientific publishing ...enterprise will benefit. We’ll have fewer students and postdocs sitting frustratedly in reproducibility jail and... PhD at the MRC LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, ..., California. She joined Elsevier after her postdoctoral studies, and has held various editorial roles... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....For more details on this technology, see our blog post Overview of AAV Encoded Calcium Sensors). We are...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog PostPublishedAug. 8, 2019, 1:19 p.m.... This post was contributed by Magdalena Julkowska, a postdoctoral researcher at KAUST, Saudi Arabia. ...Additional resources on the Addgene blog Find blog posts on scientific sharing Get tips on writing a review... submission to the subsequent journal. As a postdoctoral researcher studying plant development and abiotic...HapMap population in Amsterdam. Currently, she is a postdoc at KAUST, Saudi Arabia, in the group of Prof. Mark... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....This post was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing...outside of the lab meeting setting. Check out the blog post “Fostering Communication in Remote Collaborations...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC and... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The technique...thymine (T) to hybridize and form hydrogen bonds. This post focuses on "sticky end" TOPO (also called TOPO-TA... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....This post was contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’... detailed investigations into the release of DNA post-cut. Using substrate DNA labeled on both sides of... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....Resources Find more career posts on the Addgene blog Read our recent post on Synbio start up Revbio ... -
2022 Depositor Week
TypeBlog PostPublishedDec. 12, 2022, 2:15 p.m.... our Depositor Week schedule: Monday: Thank you post (this is it!) Tuesday: Stats roundup on Twitter...