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Showing: 241 - 260 of 515 results

  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ... the nucleotide sequence during the DNA repair process. The addition of small molecules that promote homologous...Also, because non-homologous repair is a random process, zygotes edited using the same nuclease may not...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...feedback. They’ve most likely been through the process themselves many times and will have useful advice...agency’s investment. 6. Failure is part of the process, keep your head up! It’s very likely that at first...

  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...road ahead for students interested in leading the process despite the recent advance. Similarly, grad students...biomedical research from its systemic flaws." Proceedings of the National Academy of Sciences 111.16 (2014...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...components of Cascade for maximum efficiency of the process. Various labs have designed all-in-one Cascade ...needs to remain anchored to Cascade throughout the process. The reeling of ssDNA and unwinding of dsDNA occur...

  7. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...delivery is also common. Due to the intubation, this procedure is considered quite invasive. Restraint: anesthesia...similar to its intratracheal counterpart, as both procedures target the lungs. The main differences are that...

  8. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...an offer but even as I did (successfully!), the process felt unnatural and intimidating. I felt unprepared...unhappy.   Negotiation as a creative problem-solving process Although at first glance it may appear that there...

  9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...sybody candidates. Figure 2: Outline of the process for selection of sybodies against membrane proteins...sybody), ribosome, and mRNA. During the screening process, displayed sybodies that bind to the biotinylated...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...Protein expression refers to the second step of this process: the synthesis of proteins from mRNA and the addition...Plant systems share many of the same features and processing requirements as mammalian cell expression systems...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...scientists have been able to study many cellular processes, including mitosis, mitochondrial fission/fusion...insertions can only be detected by labor-intensive processes like PCR or by evaluating visual phenotypes. Improving...

  13. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...Gene targeting takes advantage of this natural process to replace a targeted genetic locus with homologous... locus of interest. To give you an idea of the process, we’ll walk through an experiment designed to knock...

  14. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...favorite in-house protocol. DNA yields for this procedure are typically in excess of 100 ng/ul, more than... carbohydrates that can inhibit the cell lysis process. To ensure that lysis is complete, use a reagent...

  15. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...genes or entire pathways in a particular disease process. While CRISPR is certainly not the first means ... screen using CRISPR libraries is a multi-step process (see Fig. 2). In most cases, CRISPR libraries are...

  16. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...Therefore, these can be used as “timers” for cellular processes following their activation. The four main FTs ...Throwing color lights on molecular and cellular processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov ...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    .... During the propagation and/or transformation process, some bacteria will either never receive a copy...antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp only affects...

  18. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United...pseudodiploidy and high rate of genetic recombination. Proceedings of the National Academy of Sciences, 87(4), 1556...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...and without degradation during the extraction procedure. Additionally, purified DNA was free of agarose...analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences of the United...

  20. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...one is needed. For these reasons, we are in the process of transitioning our titering method from qPCR ...information needed for your PCR experiment. The process to titrate AAV by ddPCR begins with diluting the...
Showing: 241 - 260 of 515 results