...photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication... be expensive and time-consuming to repeat the process with these new photoconvertible proteins. With ...state after excitation at 561 nm. This imaging process worked well with the common GFP variants EGFP, ...

...Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide...Homologous recombination is the process by which nearly all domains of life repair genomic damage, specifically...Researchers have long taken advantage of this natural process to integrate protein tags into the genomes of S...

... ligands can elicit varying degrees of β-arrestin-2 recruitment to receptors, indicating their agonistic...fluorescent labels. In Nature Methods (Vol. 19, Issue 2, pp. 149–158). Nature Research. https://doi.org/10.1038...Allikalt et al., 2020) Impact of labeling process: The process of dye labeling, such as conjugation with...beneficial when studying dynamic processes like mitochondrial dynamics. This process, involving mitochondrial ...specimens has offered illumination on biochemical processes that are crucial in the world of biological research...result in cellular toxicity due to the permeation process. Advanced technologies in fluorescent dyes/ligands...

...Figure 2: Christina Mork collecting samples at the hot spring. Hiking all day and processing samples...hike in. Navigating challenges: collecting and processing environmental samples Since the high temperatures...And it was exciting – one night while trying to process samples at the picnic table before dark, a bison...

...containing complementary polynucleotide chains. J Virol 9(2): 394-396. Lecomte E, Tournaire B, Cogné B, Dupont...   While the VGS process isn’t new to Addgene, we are excited to share the procedures and Python scripts... vector (AAV) samples and created a simplified process, termed viral genome sequencing (VGS), to confirm...We recently published a description of the VGS process and use cases in Human Gene Therapy. Existing methods...substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...

...from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input concentration across ...laboratories from using these methods for routine processes like plasmid validation. At seqWell, Inc., our...sample-specific barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell...additional benefit of plexWell™’s sequential tagging procedure, the quantities of library molecules for each ...a wide range of input DNA concentrations. This process is explained more fully in the animated Fig. 1 ...samples are brought in line with each other before proceeding to library prep. Even with this “pre-prep” normalization...

...Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414 (2010). Cleveland Clinic. Gram Stain. https...bacteria’s thinner membrane. Procedure The stain is a pretty simple process. First, flood the heat-fixed...pneumoniae, while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After ...photos/occbio/7092385559 For more specific procedural instructions, including timing, preparing the... them red or pink. At the end of the staining procedure, gram positive bacteria are stained purple or ...

...closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique Detection...PMID: 23792628. PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet...most important steps in the CRISPR experimental process is validating edits. Regardless of which CRISPR...not an edit in the target gene. The validation process, also known as CRISPR genotyping, is critical to...

...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...one step. Challenges in the LEGO science video process One major challenge when we started was creating...laboratory. When we started, the hardest part of the process was to embed personality in pieces of plastic, ...

...affinity and specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 ...mark it as a target for other immune cells, in a process known as opsonization. In antibody-dependent complement... the pathogen the antibodies are bound to in a process known as phagocytosis.  Other functions We’re still...

...melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle...harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B: Biological Sciences 272.1577...

...strategy, etc.) and episomal expression will last ~2-7 days at moderate levels. With regard to target cells... What is a transduction?  Transduction is the process of transferring foreign nucleic acid into a cell.... Transductions are similar to transfections, processes which introduce foreign nucleic acid into cells...   Figure 1: Transduction to expression process. A viral particle enters a host cell, enters the...

...yourself greater chances for success.  Build a team of 2 or 3 Don’t go it alone.  You’ll find that no matter...background and/or connections to even begin this process. I am the cofounder and CEO of miLab, a Boston-...innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into commercial...people for guidance.  Before even beginning the process of launching a company, you need to determine whether...

...and in line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer...content. Regardless of the process, everything goes through an editing process and final check before publication...invite you behind the scenes in our content writing process, and to even get involved yourself. You probably...science, and our entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, ...recommendations. A few discussions in the review process led to quick dives back into the literature to ...and hit publish. Making content is an iterative process. We are constantly reviewing the resources we currently...

...elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA, Tris, or water? The buffer you choose ...the plasmid, and still allowing most downstream processing. On the other hand, nuclease free water is a ...

...and during your application process so you can learn more! Figure 2: The many ways to prepare for...teaching and mentoring others (see more in Figure 2). Starting early demonstrates not only your interest...

...another. You’ll need to validate that points 1 and 2 hold true under your specific experimental conditions...when (1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets...identified as your target protein, allowing you to proceed with confidence.  Share your results Ultimately...

...Without binding to the guide RNA, Cas9 cannot cut. 2. Bind to target DNA in the presence of a guide RNA...systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA guides required...scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived from...repeat regions contain sequences required for processing pre-crRNA into mature crRNA and tracrRNA binding... Overview of native CRISPR arrays and their processing for cleaving foreign DNA. A) CRISPR arrays as...

... a template, copying the base edit. Figure 2: Cytidine deamination takes place on the free strand...SpCas9 base editors with editing windows as small as 1-2 nucleotides. To reduce off-target effects associated...formation. To further decrease indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to ...repair. Black arrows indicate natural cellular processes. BER = base excision repair. Note: hypoxanthine...

...IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development of drug... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of...system, tumors “learn” how to escape it. In this process, cancer cells develop and thrive within what is...