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Showing: 241 - 260 of 645 results

  1. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... were suitable for our needs for the following reasons: Safety: Since AAVs are nonpathogenic, we are...Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors (i.e. chemogenetics...applications, we may also be able to use these comparisons to enhance our understanding of receptor physiology...

  2. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...better plan your transductions and use a range of reasonable MOIs. 2. Create a monoclonal Cas9-expression ...used existing BRAF gRNA viruses. There are a few reasons to target different regions of the gene with multiple...testament to how far genome engineering has come. For comparison, in 2007 it took about 3 months to knockout a...

  3. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    Published
    Dec. 15, 2016, 3:57 p.m.
    ...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...

  4. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution...https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, ... Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning diverse Type IV structures...

  5. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...

  6. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...heteroduplex transitions back to DNA duplex, and it’s the reason guide sequences have a maximum length of 20 bases...Sternberg et al., 2015).     Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0..., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis...

  7. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...for cloning but PX330 only uses 20 nt? A16: The reason for the 30 nt region on the upper is because the...doi.org/10.1126/science.1231143  Elliott, B., Richardson, C., Winderbaum, J., Nickoloff, J. A., & Jasin...cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318–331. https:...

  8. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...how Nextflow helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our...  Rachel Leeson...

  9. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ... research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size... Rachel Leeson...

  10. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles para sus experimentos... Rachel Leeson...

  11. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound...  Rachel Leeson...

  12. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...  Rachel Leeson...

  13. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ... of holiday spirit still linger (despite the unseasonably warm weather we had last weekend). We’ve spent...DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to town. @Addgene @UMich_...

  14. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...think of any better way to start off our holiday season than by thanking the twelve guest bloggers and ...  Rachel Leeson...

  15. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 ...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

  16. Summer Fun at Addgene!

    Type
    Blog Post
    Published
    May 12, 2022, 1:15 p.m.
    ...Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer plans this year!...                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      Rachel Leeson...

  17. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ... to #DeckTheLab with virus snowflakes from @CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make...We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance...

  18. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...introduction to plasmids with Director of Biology Lianna Swanson was very popular, with the students excited to ...different career options in science, with Abigail Anderson commenting that “There is not just a scientist...
Showing: 241 - 260 of 645 results