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Addgene

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We narrowed to 729 results for: NSI

Showing: 131 - 140 of 729 results

  1. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ... elaborated on later. As for what job fairs to consider, some of the best options are the Naturejobs Career...most relevant experience at the top. You may also consider visiting any of the employer’s career pages to... apply for the position. This post on Business Insider nicely summarizes good job fair practice. Career...’t have a particular employer in mind, you may consider starting with indeed.com or monster.com, where... important to realize that you have to take responsibility for your own professional development, not ... Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs-2014...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...provenance (this is the history of the cell line consisting of records of the origin and propagation of the...cell line contamination: Epithelial Mesenchymal Transition Is Required for Acquisition of Anoikis Resistance... The same test is so good that it is used as a forensic technique (aka DNA fingerprinting) that can place...convince people of some of the basic tenets of responsible cell culture outlined in this article. Now many...studied biochemistry at Warwick University, gaining extensive cell culture research experience through working...for a range of testing, from animal doping to forensics, DNA sequencing to drug testing. You can find ...

  3. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...includes the aforementioned internal control and is consistent with the result in Figure 1 could look like this...associated with the independent variable should be consistent over time and from different preparations of ...replicate control is important, it speaks to the consistency of the reagents and hands performing the experiment...experiment, not the reproducibility or consistency of the observed phenotype associated with the independent...replicates control for the reproducibility and consistency of the observed phenotype associated with the...may be, proper controls are a basic tenet of responsible scientific inquiry and investigation. References...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...express our gene of interest. This element is responsible for initiating the transcription of your insert...eukaryotes have muliple polymerases which are each responsible for a specific subset of RNAs. To gain this specificity...transcript     Description Expression Additional considerations CMV General expression mRNA Strong mammalian...elongation factor 1 alpha Constitutive  Tends to give consistent expression regardless of cell type or physiology... used for Description Expression Additional considerations T7 in vitro transcription/ general expression...regulatable  Often paired with the temperature sensitive cI857 repressor. T3 in vitro transcription/general...

  5. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...these particularly energy-intensive systems in a more environmentally responsible manner.  Other ways to ...service items we employ dozens of pieces of energy-intensive lab equipment on a daily basis. By the beginning...100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown announced...substantially reduced in an attempt to save energy. The transition to 100% renewable electricity will allow us to...

  6. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...synthesized enzyme. Biotin protein ligase (BPL) is responsible for this highly specific post-translational protein...biotinylated. In E.coli for example, the BPL, BirA, is responsible for biotinylating only one lysine in the entire...Esmond E. Snell was able to purify the protein responsible for binding biotin, avidin. Avidin was aptly ...Kresege et al., 2004). Avidin is a glycoprotein that consists of four identical subunits that can each bind ...non-selectively label DNA and RNA. Photobiotin acetate consists of a biotin molecule attached to a photoreactive...Photobiotinylation is sometimes easier and less expensive than other enzymatic methods. Scientists have ...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Faculty Position. This edition has substantial expansions on the chalk talks and Zoom interviews sections... and mentoring to several people that found my insights helpful. All went on to get faculty positions ...understand your proposal to give you proper consideration. 3. Present an easy to follow seminar See item...Implications for human disease" is vague. Instead, consider "A mechanistic understanding of this system will...free!) contains strategies, hints, anecdotes, and insights. After I wrote the first version of my manual,...publish this manual and earn some cash for my expensive habits of running shoes and single malt whiskies...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...being realized. There are a variety of reasons to consider using genome editing to change the genetic code...sequence and ~75 nucleotide scaffold sequence) is consistent between plants and other organisms, but the promoter...CRISPR components can be expressed stably or transiently depending on the delivery method and cell type...CRISPR components can be delivered and expressed transiently using a standard detergent, Polyethylene Glycol...GOI) into plant cells. A) The system typically consists of a Ti plasmid, into which the GOI is inserted...deliver CRISPR-Cas9 to plant cells and should be considered alternative to PEG or agrobacterium-mediated ...

  9. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...change on the opposite strand). Both are base transition editors: converting one purine base to the other...pyrimidine base to the other. Figure 1: Base transition editors catalyze purine-to-purine or pyrimidine-to-pyrimidine...BioRender.com. How does base editing work? Base transition editing requires three elements. They are, broadly... in David Liu’s lab in 2016. History of base transition editing Cytosine base editing The beginnings ...frequency of 1.2%. Although the lab did not conduct comprehensive genome-wide studies of ABE specificity, their... a similar approach. Dali Li’s lab’s A&C-BEmax consists of a fusion of both cytidine and adenosine deaminases...The other 8 base-to-base changes require Base Transition Editors, which will be discussed in another post...

  10. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ... concern, or nothing to worry about all. Let’s consider several scenarios below for a CRISPR experiment...An off-target rate of 5% is far too high when considering human patients and the diseases associated with...many more. While such software typically only considers DNA sequence and doesn’t take into account chromatin...the genome to reduce off-targets, it is time to consider your Cas enzyme. Even if there are similar sequences...unnecessary for many applications, it is the only comprehensive method available. Targeted sequencing approaches...must be quantified slightly differently. For a comprehensive approach, RNA-seq would be an appropriate method...
Showing: 131 - 140 of 729 results