We narrowed to 842 results for: Eras

...soon find yourself encountering flow data in the literature. Data reported from flow cytometry experiments...work is done in flow cytometers, and output is generated through measurement of events. An event is something... machine is plotted as a dot on an X,Y axis to generate a dot plot (Figure 1). Clustered events are called...                         Figure 1: While the plot generated often looks like the graph on the left, it might...intersection of X,Y. For flow plots, however, that is generally not true. Because of the variations in fluorescent...Of course, this is quite easy to do on a plot generated in Biorender! While a helpful visualization tool...

...genotypic and phenotypic artefacts. The life science literature is a litany of false interpretation in cell culture...Down-Regulation of HIF-1α by NDRG2 Expression Attenuates Proliferation and Invasion in Renal Cancer Cells, Marker-independent...out by the commercial repository. Repositories generally ensure cell line identity and purity making it...to the original as possible and so any results generated are reproducible and reliable. When you order ...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate...identity. The Nature Publishing Group recently reiterated its strict stance on the matter. Funding bodies...

...that only 11% of the academic research in the literature was reproducible by their groups. Finally results...copes with the growing amounts of digital data generated in labs. While many labs approach the situation...are available on the market today, ranging from general note taking tools, to more scientific field-based...From pictures to excel sheets, researchers today generate vast amounts of digital data during their work...within your lab? In every lab, there are certain hierarchies and restrictions to data access. If these are...walk you through your ELN’s features and this interaction will give you an idea of how responsive and supportive...

...member at Albert Einstein College of Medicine. Several sets of near-infrared fluorescent proteins (NIR...the NIR light coming from your source and to your camera.  Two-photon image of miRFP670nano3-expressing...reported widely used dimeric iRFPs (Table 1). In general, the monomeric state of a probe leads to less interference...al., 2018). For detection of protein-protein interactions, we recommend using NIR split reporters based...detect spatiotemporal dynamics of protein-protein interactions. Nat Methods 11, 641-644 (2014). Filonov, G....reporter for in vivo imaging of protein-protein interactions. Cell Chem Biol 20, 1078-1086 (2013). Shcherbakova...

...candidate regulatory kinase is known, it’s possible to generate non-phosphorylated recombinant protein and perform...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...position. Practical tips for your experiments To generate as much pSer-incorporated protein of interest ...be optimized for parameters such as induction temperature and length of induction, which will vary between...between different proteins. Beyond these standard considerations, it is important to determine how much phosphorserine... effects of pSer on enzyme function, protein interaction, etc. by comparison to non-phosphorylated or ...

...suggests there are hundreds. This only includes general lab strains designed for subcloning or protein ...ATPase that is essential for recombination and general DNA repair Reduces plasmid recombination, increases...Δleu mtl1 DH10B Streptomycin MC1061 derivative. General cloning and storage, blue/white screening, leucine...)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ- DH5alpha   General cloning and storage of common plasmids, blue/white...galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ- JM109   General cloning and plasmid maintenance, blue/white screening...(TetR) TOP10 Streptomycin MC1061 derivative. General cloning and storage, blue/white screening. F- mcrA...

...produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI, and various fluorescent proteins... elucidating the molecular mechanisms behind interactions between crops like potatoes and their microbial...that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass. Dr. Duedu would...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to test ...

...unpredictable loud noises and flashing lights - that sent average, control mice into depression. In case you were... skeletal muscle expression of kynurenine aminotransferases, to enhance the conversion of kynurenine into...between the muscle and brain, which may have therapeutic implications, say Hyo Youl Moon and Henriette...psychiatric and cognitive conditions as well as neurodegenerative diseases.” I don’t know about you, but I think...Pettersson AT, Ferreira DM, Krook A, Barres R, Zierath JR, Erhardt S, Lindskog M, Ruas JL. doi: 10.1016...

...for general audiences. So far, we are beginning to make an impact on society and science literacy. We ... Addictive Brain and science communication for general audiences. How does The Addictive Brain address...science, especially disease related topics. Neurodegenerative and brain disorders are frequently overshadowed...“manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked...

...become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke University Medical Center...that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis. For...Rather, they relied on published studies in the literature to curate the perfect library, obtained the appropriate...researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene. “We called... cancer therapy...

...that in January. This involves going in front of a camera and kind of walking them through the steps of, ... heart to look at the inside. We do a lot of interactives and videos, as well, to supplement the hands-on... Caitlin also collaborates with the sales and operations departments at eScience Labs. This can involve...eScience warehouse is onsite, Caitlin can visit the operations team to make sure “that everything that’s going...facilitator between her team, the sales department, and operations department with the ultimate goal of determining...

...that Addgene is officially filed, recognized and operated as a nonprofit under the United States Internal...fulfilling our mission. “Addgene’s nonprofit mission: Accelerate research and discovery by improving access to...in that we are able to fulfill our mission by generating revenue through distribution of research materials...nonprofit" is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution...nonprofit, open science and Addgene in our mission to accelerate research with easier sharing and collaboration...

...Administration of Substrate: Some luciferase reporters, such as Renilla luciferase (RLuc) or NanoLuc, require...bacterial reporters that use fluorophores and luciferases to track bacteria in real time, but each of these...Blog Learn more about in vivo imaging tools The luciferase reporter gene The green fluorescent protein reporter...vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein biosensors ...

...saves time, knowledge and makes your insights discoverable. Watch this quick video to learn how ReFigure...How much time do you spend reading scientific literature? Very often the data of interest are in 1 or ...preclinical and clinical data about a disease or therapeutic Side-by-side comparison of the same experiment...save analyses with the relevant figures from a literature review. No more hunting through a whole folder...reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology...

...Plasmids 101 will provide you with an overview of general molecular biology knowledge and techniques, and...Site (MCS) Short segment of DNA which contains several restriction sites allowing for the easy insertion...later work.     How do scientists use plasmids? Generally, scientists use plasmids to manipulate gene expression...Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular... viruses that can be used in research or for therapeutics Addgene has compiled various educational resources...

...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...the receptors are named after the isotypes they interact with, so IgG isotypes bind to Fcγ receptors; IgE..., ADCC can be induced through a very similar interaction between IgE antibodies and eosinophils, where... induce?  Fc receptors are intriguing from a therapeutic standpoint. Engineered monoclonal antibodies ...phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI:10.1126/sciadv.abl9171...

..., functions at temperatures up to 43 °C and can be used for genome editing in moderate thermophiles.  ...straightforward workflow for doing gene targeting, reporter generation, controllable gene knockouts, and inducible gene...Strategy for leak-free transcriptional control. C) Iterative transduction workflow overview. Image from Sun...successfully targeted cells that can be used to generate organoid cultures with a modification of interest...

...by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and two untagged... exhaustive refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16...treated with the luminescent enzyme substrate coelenterazine, followed by the addition of the endogenous...

...vivo (try pcDNA3-EGFP). If you can get away with generating transiently transfected cells, you will not need...that you will need accessory packaging vectors to generate the virus. Bacterial vectors This system is great...binding or folding assays. Yeast vectors There are several types of yeast expression vectors. Consider your...microscope, we offer a collection of vectors carrying several fusion proteins. Pick your promoter, selection ...promoter (e.g. pMT-puro). Worm vectors There are several approaches to studying YGOI's function in worms...

...peptides consist of as little as 8 or as many as several hundred amino acids, depending on the tag, and ...charged amino acids. FLAG tag has been used with moderate to high success rates across all applications....including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide... has two negatively charged amino acids for an overall negative charge. This tag has been used successfully...Additional resources on Addgene.org Addgene’s General Use Antibodies Antibody guide Antibody protocols...

...chromosomes are represented by two to three copies, but several have four. No Y chromosome is present in this line... line, making it genetically female. HEKs are generally cultured as an adherent monolayer, though derivatives...plasmids with the SV40 origin of replication. To generate a virus, you need only transfect the ‘helper’ ...the way up to the production of clinical viral therapies. Protein production  HEKs are extremely easy ... and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells can catch...

...pluripotent state. Though a valuable tool for regenerative medicine, direct reprogramming is an inefficient...demultiplex the scRNA-seq data and build multi-generational cell lineage trees. CellTags were detected over...MettI7a1 encodes an uncharacterized putative methyltransferase. Adding Mettl7a1 to the standard Foxa1-Hnf4α... at Addgene. The Morris Lab's website also has several useful resources including a link to a detailed...methods for delivery of reprogramming factors for generating iPSCs Learn how fluorescent proteins help researchers...

...zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger... for Synaptic Imaging However, the first two generations of iGluSnFR had limitations: they were not optimal...extrasynaptic glutamate release. Building on the previous generation of the SF-Venus-iGluSnFR (itself an improvement...not neurons). iGluSnFR3 is also available in several ready-to-use viral vectors.    References and ...

...SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence and ultimately its degradation... plant protein TIR1 in non-plant cells, which interacts with the cells’ endogenous ubiquitin ligase, the...with mAID. Using this method, the lab was able to generate conditional alleles of a few essential proteins...attempted to engineer loops to create genomic interactions that wouldn’t normally occur. The synthetic ...enhancer. What makes LADL unique from previous iterations is the addition of CRY2, another Arabidoposis...

...encoded fluorescent executioner caspase reporters. Several apoptosis reporters have been developed, such as... and fluorescence based reporters however have several limitations. First, FRET signals are often difficult...They then optimized the length of both linkers to generate a reporter that would fluoresce: FlipCherry. FlipCherry...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...Cabantous, Stéphanie, et al. "A new protein-protein interaction sensor based on tripartite split-GFP association...

...responsible for a phenotype can be identified by next-generation sequencing of the genomic DNA. However, some ...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter...approach to expand our understanding of ApoB-LPs and accelerate the discovery of additional genes and small-molecule...tool should serve as both a powerful tool for generating complex regulatory systems and further the study...nematode Caenorhabditis elegans. They have found several voltage biosensors and conditions that can be used...

...successful mutation of your favourite gene, there are several different methods available. Thankfully, there ...validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the gold standard...emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will...desired genome edit. Since exploitation of NHEJ to generate translational reading frame-altering indels for...validation methods As mentioned, these assays have several major advantages over the commonly employed mismatch...

...cleavage promotes collateral cleavage of other RNAs. As with other Cas13s, this collateral cleavage does ...and Yan et al. describes new Cas13d enzymes that average only 2.8 kb in size and are easy to package in ...possibilities. To identify Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR ...accessory protein RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased...

...and fostering team success. This shift requires deliberate preparation and a willingness to embrace new ...the room," understanding team motivations, and leveraging tools like the Myers-Briggs Type Indicator or...technical and managerial roles, and I've witnessed several colleagues do this while changing industries and...Courses: Leadership programs on platforms like Coursera, LinkedIn Learning, or edX. Podcasts: HBR IdeaCast...Scientists: What Makes a Good Leader Anyways?  Transferable Skills Guide: Managing a Team  ...

... a structural precursor protein, and Pol is a polymerase. Envelope gene (may be pseudotyped to alter infectivity...γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis...packaging cell line. For example, Phoenix, a second generation γ-retrovirus packaging cell line developed by...region of the 3’ LTR and are primarily used in gene therapy research to reduce promoter interference and/or...More resources on addgene.org Addgene Protocols: General Transfection The Addgene Retrovirus Guide  ...