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Showing: 521 - 560 of 788 results

  1. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...can almost be boiled down to one, key concept: Creating a culture of excellent, effective communication...practice and training. This is important because it creates a culture of engagement in the issues at hand and...

  3. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ... are we! This expansion will give researchers increased flexibility when planning their research and expanded...This split allows us to provide researchers with increased choice and flexibility; while ensuring we can ...

  5. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... to GRABNE, and having both colors allows for increased options for multiplexing in experiments, including...both nLightG and nLightR showing significantly increased selectivity for norepinephrine over dopamine. ...

  6. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...ahold of your destinies” and also suggested some concrete actions that can be taken now to solve some of...the possible science careers. She advocated for increasing the value and priority put on good mentoring ...

  8. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...operons from two different bacteria, Bourdeau et al created two constructs optimized for expression in E. coli... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

  9. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...DNA-protein interactions, the reliance on fixation does increase the chance of false-positives, as proteins may...RUN doesn’t require fixation and significantly decreases background noise, reducing the read depth necessary...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ... to help you do better research. CRISPR is an incredibly fast-moving field, and we want to make it easy...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  12. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...accurately reflect our culture and outlook: Addgene increases productivity by providing world-wide access to...leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters a culture...

  13. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...submicromolar) DA affinity and high-magnitude responses (increases in fluorescence) at low concentrations (e.g., ...The GRABDA dopamine sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: ...

  14. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  ... mouse-derived single-domain antibodies. Image created in BioRender and adapted from Xu, et al., 2021....

  15. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA, for example...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

  17. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...leader’s job to provide the driving inspiration, to create a productive culture and to correct systemic problems...try operating in a different role?  I think the secret sauce can be summed up in one word – initiative...

  18. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... bacteria and a mammalian selection cassette to screen for positive transfected cells. The addition of...your applications and experimental design. You can create a new strain with a disrupted gene by transforming...

  21. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ...out-of-the-box ideas that might jump start your learning. 1. Create a peer mentoring group We don’t all get the mentoring...that there’s some correct level of intimacy that creates the magic of a safe space. No matter why, now is...

  22. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...into the collection and to start shipping. They created the best safety protocols they could with limited...While many were sympathetic to this need, we're incredibly grateful that the Chan Zuckerberg Initiative ...

  23. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...different biochemical secondary energy sources to increase protein yields from 36 μg/mL to 266 μg/mL. The...oxytetracycline enzymes. Next, this system was used to recreate natural biosynthetic pathways. Biosynthetic pathways...

  24. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...empirical essays had also stirred some of my more creative faculties into motion, such that a desire to once...filmmaking, podcasting, philosophy of science, creative writing, designing and curating exhibitions, animation...

  26. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...temperatures, but you may need to increase your primer length and increase the Tm if you are trying to clone...

  27. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...This recessive genetic condition leaves patients incredibly sensitive to sunlight and prone to developing... they now plan to use CRISPR-Cas9 technology to create cell lines and/or mouse models carrying different...

  28. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...perspectives Develop coaching skills Encourage your creative thinking Enhance leadership skills Support inclusion...

  29. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...across the hall, fields, and other professions increases your chances of finding a well-suited job. EAs...of qualified scientists entering the workforce increases, EAs allow graduate students and postdocs an opportunity...

  30. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...easier. NEBNext Ultra FS DNA Library Prep Kit To create a DNA library, one must first fragment a DNA sample...snacks. At the end of the workshop, all our newly created libraries were analysed to see how well the kit...

  31. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...two iodine residues. Compared to MG, MG-2I has increased ROS generation when bound by the dL5** FAP.  The... KillerRed requires mitochondrial targeting to increase cell ablation efficiency, but dL5** phototoxocity...

  32. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translationally silent. Both of these methods increase our understanding of translation dynamics, but...but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple hairpin...

  33. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...advances in technologies that permit large-scale creation and synthesis (“writing”) of longer pieces of ...samples from several plates are pooled together to create a single library containing fragments carrying ...

  34. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet, and shocking...

  35. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...in China was using CRISPR/Cas9 in an attempt to create gene-edited babies. Several hours later, the scientist...the media and academic conference response to He created a positive feedback loop that rewarded unethical...

  38. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

  39. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...genetic diseases As CRISPR technology becomes increasingly more cost effective and precise, researchers...influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists are ...

  40. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    Published
    April 14, 2016, 2:30 p.m.
    ...mutagenesis to extend at different pH values. Using a screen that takes advantage of the fact that coiled R-...both lower and higher pH. We initially used this screen to find R body mutants that couldn’t extend at ...
Showing: 521 - 560 of 788 results