We narrowed to 608 results for: Sis

1. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...Goedhart and Marieke Mastop! Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...degradation. Additionally, electron microscopy analysis is time consuming. GFP-tagged Atg8p or LC3: ...

3. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...salary information as of December 1st. Salary inconsistencies across geographical regions When we originally...

4. Antibodies 101: Selecting the Right Antibody

   Type

   Blog Post

   Published

   March 15, 2022, 1:15 p.m.

   ...papers you could get your hands on, formulated a hypothesis, and planned out your key experiments. Unsurprisingly...

5. Antibodies 101: Secondary Antibodies

   Type

   Blog Post

   Published

   Dec. 21, 2021, 2:15 p.m.

   ... assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification ...

6. Antibodies 101: Conjugation

   Type

   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ...you can often remove the incompatible agents by dialysis or gel filtration.    There is, of course, a lot...

7. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...removed. Degron tag systems include small molecule assisted shut off (SMASh), auxin-inducible degron (AID)...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...phytochrome-like fusion proteins from the plant Arabidopsis thaliana. In the presence of red light (650 nm...

9. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ...encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (2016): 19575. PubMed PMID...

10. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ..., nanobodies, which occur naturally in camels, consist of a single heavy chain, and they are small and...

11. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...foundation for postdoc-related data collection and analysis. He is also a strong supporter of science Master...

12. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

13. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...rapidly changing landscape of genetics and our consistent stream of events mean that there is never a dull...

14. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic Biology with ...

15. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ... codon-optimized  cryptochrome 2 (CRY2) from Arabidopsis thaliana. When exposed to blue light, CRY2 undergoes...

16. Fluorescent Proteins 101: Introduction to FRET

    Type

    Blog Post

    Published

    June 27, 2017, 1:03 p.m.

    ...contribute to our understanding of diseases and even assist in drug discovery. References 1. Baird, Geoffrey...

17. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    Published

    July 11, 2019, 1:26 p.m.

    ...positive selection marker such as an antibiotic resistance gene is also included to select for successfully...

18. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type

    Blog Post

    Published

    July 23, 2020, 1:20 p.m.

    ...Xiong Z, Yang J, Xu Z, Huang Z (2017) Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein...

19. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type

    Blog Post

    Published

    March 12, 2020, 1:15 p.m.

    ...: Create promoters with the help of epigenetic analysis and designed using stretches of DNA that were ...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...backbones expressing blasticidin or puromycin resistance genes.  It’s that simple! Good luck and happy...

21. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...goal to develop low cost and quick point-of-care diagnosis kits (reviewed in Tsang and LaManna, 2020). To...

22. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...integration within the target cells, ensuring more consistent and reproducible results in calcium assays. There...

23. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic...

24. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ...plant biology to achieve traits such as disease resistance. MoClo is a great tool to deliver many combinations...

25. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...xCas9 was generated by the Liu lab through phage-assisted evolution, specifically with the goal of generating...

26. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...a prime editing guide RNA (pegRNA). The pegRNA consists of a CRISPR gRNA extended to include a primer ...

27. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...Shah's and Jim Well's lab induces cre-dependent apoptosis. Now available as a ready to use viral prep in...

28. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...proof-of-principal study were very helpful in assisting me with applications for much larger fellowships...

29. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...access to rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet...

30. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ...literature review, survived technical feasibility analysis, and was a marketable product. It was a small ...

31. Transferable Skills Guide: Managing a Team

    Type

    Blog Post

    Published

    Jan. 30, 2018, 2:42 p.m.

    ... happening even though I met with X on a weekly basis! The silence and maybe even the slight awkwardness...

32. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type

    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...available to immunologists, allowing for the rapid analysis of cell populations within a heterogenous tissue...

33. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type

    Blog Post

    Published

    June 25, 2024, 1:15 p.m.

    ...a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence...

34. Pooled Library Amplifications

    Type

    Blog Post

    Published

    May 30, 2024, 1:30 p.m.

    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

35. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ... & Lopez-Berestein, G. (2020). A retrospective analysis for different routes of administration in Mice-Percutaneous...

36. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ...inside insect cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus...

37. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ...explore Myc’s role in cell proliferation and tumorigenesis.  Using two different synthetic peptides corresponding...

38. PhD Applications After COVID

    Type

    Blog Post

    Published

    Jan. 5, 2023, 2:15 p.m.

    ...in-person events change seemingly daily (and are inconsistent between universities!) This guide tells you ...

39. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...., 2000). Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic...

40. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    Published

    April 19, 2016, 2:30 p.m.

    .... An imperfect solution is to restrict imaging analysis to cells expressing the lowest levels of the fusion...