We narrowed to 740 results for: NSI

...pairs were easily visualized and results were consistent with copy number variation (CNV) analysis of ...

...bedside is, in addition to thorough ethical considerations, in making sure that the research has been ...

...post has helped clarify some of the important considerations when using, amplifying, and verifying pooled...

...into a specific bacterium, it can be important to consider the type of bacteria it is being used in, and ...

...requested and incredibly useful tools, they require extensive background work from the requesting scientist ...

...the break and 91bp on the PAM-proximal side, consistently supported higher frequencies (up to 60%!) of...

... The Addgene Advisory Board consists of prominent members of the bioscience and tech transfer communities...

...proteins to be imaged together, you will need to consider the same factors as when choosing an individual...

...decide on the best way to structure roles and responsibilities as we grew.” Initially, he spent the majority...

...Resources References Aggarwal, A, et al. (2025). A sensitive orange fluorescent calcium ion indicator for imaging...

...unsure what amount of antibody to use, you should consider using an isotype control. Pro tip! Many companies...

...packaging of the virus into particles. E2A/E2B are responsible for viral replication, with E2B being the primary...

...questions to be clarified, especially with regard to licensing models. Direct sharing of work from universities...

...lox sites. This system takes advantage of the extensive supply of transgenic reporter lines that supply...

... plasmid. By leveraging our core strengths of intensive screening and the use of high throughput platforms...

...Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective...

...succeed in communicating. Even the most rigorous, insightful, and novel scientific research will be wasted...

...RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans research to study gene function...

...alone. A few years ago when I started my career transition from bench science to software product management...

...Please note that NGS verification is more time-intensive than Sanger sequencing or the other techniques...

...function of immune cells that are not excitable but responsive to calcium. We’d love to expand our cancer and...

...oxygen. Other useful backbone modifications to consider include amide linkages, unlocked nucleic acids...

... live imaging application. Each visual barcode consists of a fluorescent protein coupled to a specific...

...backing of SnapGene’s sequence viewer software and extensive feature library, our updated plasmid and sequence...

...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...

...making your plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving...

...terminator. These relatively large cassettes (considering the gRNA itself is ~100 bases) limit the number...

... activator-like effector nuclease (TALEN) kit consisting of 834 plasmids. The researchers showed they ...

... with SpyTag/SpyCatcher, allows for iterative expansion DogTag/DogCatcher SpyTag/SpyCatcher Rapid covalent...

...and in vivo laboratory experiments ranging from transient gene expression in a particular cell type to CRISPR...

...completely normal to be anxious, nervous, and/or apprehensive. I still get nervous quite regularly, and I'...

...or Jay Bradner ([email protected]). Considerations when getting the dTAG system up and running...

...genes of interest. These transcription factors consist of the GAL4 or ZIF268 DNA binding domains fused...

...samples you may have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of...

...did not just automatically come to a solution. Consider the thoughts that went through your head as you...

...Lerner RA, Wigler M (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae...

...Russell, P., & Guttman, M. (2014). Methods for comprehensive experimental identification of RNA-protein interactions...

... new growth phase of the organization and the expansion of our impact, I’d like to take a moment and honor...

...funding bodies and review committees encourage and consider preprints as demonstration of productivity. Part...

...resources. There are three types of cognitive load: intrinsic cognitive load is the effort associated with a...

...exonic gRNAs. In fact, the method is specific and sensitive enough to detect gene copy number based on the...

...salary information as of December 1st. Salary inconsistencies across geographical regions When we originally...

...complementarity to the gRNA, they have a higher propensity to reform double helices and decrease Cas9 cutting...

... to train our young scientists for futures both inside and outside of academic research (4). There are...

...assembling the toolbox needed for anyone to begin considering genetic engineering projects in a very big way...

... a number of different options. By carefully considering your ncRNA of interest, you should be able to...

...Scripps Research, where he works on genetic code expansion.  References and Resources References Weber E...

...Off-target effects are also important safety considerations for all CRISPR therapies, but especially concerning...

...Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang Ligation Fidelity...

...functions of key regeneration-related genes in four inexpensive and commercially available planarians: Girardia...