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Showing: 651 - 700 of 704 results

  3. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...protein in low abundance or whose conformational state may change. If your protein might become slightly...

  6. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...

  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ... allows users to test a variety of different orientations and components within the system to maximize...

  11. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...targeted to precisely edit with the goal of ‘fixing’ mutations or introducing a desired edit, but this particular...

  12. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination...

  16. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...

  18. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

  19. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...

  23. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

  25. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...their comfort zone around 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially...

  27. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

  28. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...more important to you and why? What are their expectations/needs and how are they different from mine? ...

  31. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...disulfide-activated ligands. Due to the concerns of off-target mutations and transgene integration in crops, delivery of...

  32. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ... on the Addgene Blog DIY DNA ladders from Penn State University Use Nanobodies to Control Protein Activity...

  37. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... edits. Their final tool, ACBE-Q (for an N108Q mutation in the deaminase), produced an average A → C product...

  40. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...calcium indicator jGCaMP7, that are optimized for excitation wavelengths above 1,000 nm and enable two-color...

  42. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    Published
    Jan. 23, 2020, 2:45 p.m.
    ...provides transparency to everyone involved about the status of each project. This creates accountability on...

  48. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ...are going to be there – undergraduate students, rotation students, joining a new lab at any career stage...

  50. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ... Q, Liu Q, Huang W, Li X, Wang Y (2017) Current status on the development of pseudoviruses for enveloped...
Showing: 651 - 700 of 704 results