We narrowed to 810 results for: nin

1. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

   Type

   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...double-strand DNA break repair, if non-homologous end joining (NHEJ) disrupts the gRNA target site after the ...Targeting. Front Neurosci 11: . https://doi.org/10.3389/fnins.2017.00503  Chamberlain K, Riyad JM, Weber T (2016...

2. Viral Vectors 101: Optogenetic Tools

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   Blog Post

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   June 27, 2023, 1:15 p.m.

   ...light, this channel activates neuronal activity by opening and allowing positively charged ions to rapidly...You’ll need to test for these differences when designing your experiment.  Finding the right information...

3. Plasmids 101: Using Transposons in the Lab

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   Blog Post

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   July 6, 2021, 1:28 p.m.

   .... For example, piggyBac and Tol2 are best for screening promoters and enhancers since they are biased ...guided transposons in bacteria sets the stage for combining the super powers of CRISPR and transposons to ...

4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   Blog Post

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   Nov. 2, 2021, 1:15 p.m.

   ...recombination for every lot of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing...thoroughly tested in the researcher's system before beginning large scale experiments. This will help to ensure...

5. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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   Blog Post

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   Oct. 17, 2019, 12:28 p.m.

   ...flashes light of specific wavelengths, selectively turning neuron activity on or off (Atasoy et al., 2008;... genOway, we are able to distribute materials containing FLEx technology. Read the genOway press release...

6. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ...assays. Here is a simple to-do-list to follow when planning epitope tagging experiments: Retrieve Ensembl...can be epitope-tagged in a couple of weeks by combining our “TAG-IN” design tool with the csRNP method...

7. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   Blog Post

   Published

   Nov. 6, 2018, 1:12 p.m.

   ...differentiating the cells into the correct lineage and returning them back to the patient. In this post, I have...and publishing after completing her postdoctoral training from Massachusetts General Hospital and Harvard...

8. New Western Blot Protocol Video!

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   Blog Post

   Published

   March 28, 2023, 1:15 p.m.

   ...Running a Western blot can be a bit daunting for first-timers. That's why we gave our written protocol...

9. Plasmids 101 eBook 4th Edition

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   Blog Post

   Published

   Sept. 5, 2023, 1:15 p.m.

   ...from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The fourth...

10. Beginner's Guide to Viral Vectors

    Type

    Blog Post

    Published

    June 2, 2017, 2:30 p.m.

    ...guide and use it for quick reference when you're designing your next virus experiment. Subscribe to Viral...

11. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

    Type

    Blog Post

    Published

    April 8, 2021, 7:25 p.m.

    ...ready-to-use viral preps, pooled libraries, kits, and cloning grade DNA!     This is just the first of many ...

12. Addgene’s First AAV Data Hub Challenge

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    Blog Post

    Published

    Sept. 20, 2022, 1:15 p.m.

    ...after the competition, where you can get all your burning AAV questions answered.  Interested? You can learn...

13. 'Tis the Season to #DeckTheLab

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    Blog Post

    Published

    Dec. 2, 2016, 3:30 p.m.

    ...that will warm up the entire lab. We’ll choose 4 winning submissions and deliver the prizes at the start...

14. The Twelve Days of Antibodies

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    Blog Post

    Published

    Jan. 6, 2025, 5:17 p.m.

    ...to me…  A recombinant Anti-DYKDDDDK!   🎶On the ninth day of Antibody, Addgene gave to me… A neuroscience...

15. Introducing the Addgene Antibodies 101 eBook!

    Type

    Blog Post

    Published

    Oct. 29, 2024, 1:15 p.m.

    ... you to easily access relevant information for planning, executing, and troubleshooting your experiments...

16. Meet Aavery, Blugene's New Virus Friend!

    Type

    Blog Post

    Published

    Feb. 13, 2020, 2:34 p.m.

    ...you to help us make the decision between some meaningful names…. Watch the video to find out the final...

17. Suggest A Plasmid You'd Like to Find at Addgene

    Type

    Blog Post

    Published

    March 16, 2016, 5:30 p.m.

    ... deposit. However, with all the great science happening everyday, it’s not possible for us to reach everyone...

18. The 12 Days of CRISPR: 2021

    Type

    Blog Post

    Published

    Dec. 28, 2021, 2:15 p.m.

    ...delete and replace long genomic sequences. On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform...

19. The 12 Days of CRISPR

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    Blog Post

    Published

    Jan. 3, 2023, 2:15 p.m.

    ... the 12th day of CRISPR, Addgene gave to me: Noninvasive assessment of gut function using transcriptional...

20. New FLIS Plasmid (April Fools 2022)

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    Blog Post

    Published

    April 1, 2022, 1:15 p.m.

    ...2022) A plasmid-based system for locating and containing escaped flies. Nature 4:1-22. https://doi.org...

21. 2021 Deck the Lab Winners!

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    Blog Post

    Published

    Jan. 25, 2022, 2:15 p.m.

    ... Team at the Centre.     Lab support team @JohnInnesCentre rule pic.twitter.com/WMb97fpUOI— Sam Mugford...

22. The Codon Guide to Synthetic Biology

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    Blog Post

    Published

    Sept. 14, 2023, 1:15 p.m.

    ... McCarty is the editor of Asimov Press and is designing a genetic engineering curriculum at MIT. He also...

23. Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew

    Type

    Blog Post

    Published

    Feb. 6, 2018, 2 p.m.

    ... joys and difficulties of getting a lab up and running, and learn a little bit about what it was like ...

24. Addgene’s GIPHY Page is Here to Help You Express Yourself (and Your Gene of Interest)

    Type

    Blog Post

    Published

    Aug. 17, 2021, 1:15 p.m.

    ...working hard to create the most informative and entertaining protocol and explainer videos for you to level...

25. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type

    Blog Post

    Published

    Oct. 8, 2019, 12:33 p.m.

    ...and we have already collected over 100 entries spanning six different species and dozens of different ...

26. Antibody Data Repositories and Search Engines

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    Blog Post

    Published

    Oct. 31, 2024, 1:15 p.m.

    ...add it to the table! And if you're interested in joining discussions about improving antibody quality throughout...

27. Bringing Renewable Energy to Addgene

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    Blog Post

    Published

    Aug. 27, 2019, 1:08 p.m.

    ...energy-intensive lab equipment on a daily basis. By the beginning of September all will be powered by 100% renewable...

28. Addgene Protocols Go Viral: AAV Purification and Titration

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    Blog Post

    Published

    June 13, 2019, 12:19 p.m.

    ...the identity and purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research...

29. Deck the Lab 2022!

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    Blog Post

    Published

    Jan. 17, 2023, 2:15 p.m.

    ...entries, select up to three winners, and reveal the winning entries on Twitter and Instagram! Happy decorating...

30. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type

    Blog Post

    Published

    Dec. 8, 2015, 3:30 p.m.

    ... to specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time...

31. Summer Fun at Addgene!

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    Blog Post

    Published

    May 12, 2022, 1:15 p.m.

    ...Summer SciComm Series From June-September, we’ll be running a Summer Science Communication Series. We’ll be...

32. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type

    Blog Post

    Published

    Nov. 15, 2016, 3:30 p.m.

    ...explains how gene drives work and what work he's planning on doing with CRISPR-based gene drives focusing...

33. New Viral Vectors - Spring 2025

    Type

    Blog Post

    Published

    April 29, 2025, 1:30 p.m.

    ...support a wide range of experimental designs. Here are nine great additions, including our first far-red fluorescent...

34. New Viral Vectors - March 2024

    Type

    Blog Post

    Published

    March 5, 2024, 2:15 p.m.

    ... which contains a biosensor that detects both serotonin and serotonergic drugs. Check out these new vectors...

35. Interview with Bart Newland, Addgene’s Newest Board Member

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    Blog Post

    Published

    Aug. 3, 2021, 1:15 p.m.

    ...reader of science and non-fiction. I just love learning.  This interview was edited for clarity....

36. Addgene’s Blog Gets a New Look!

    Type

    Blog Post

    Published

    Jan. 25, 2021, 5:54 p.m.

    ...easily find what you’re looking for. Whether you’re joining us for the first time or you’ve been reading the...

37. Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana

    Type

    Blog Post

    Published

    Dec. 20, 2016, 3:30 p.m.

    ... recently visited Cambridge to spend some time learning and doing research at Novartis. Listen to learn...

38. Editor's Choice, October 2016

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    Blog Post

    Published

    Nov. 4, 2016, 2:30 p.m.

    ...enacted labor laws will likely increase postdoc earnings in the U.S. This increase, while overdue in my...

39. Addgene’s Top 10 Blog Posts from 2014

    Type

    Blog Post

    Published

    Dec. 16, 2014, 8:01 p.m.

    ... also loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by...

40. Addgene Stands with the Scientific Community

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    Blog Post

    Published

    April 23, 2025, 1:30 p.m.

    ...creativity in the face of funding challenges. By strengthening our commitment to collaboration and resource...

41. What's New in CRISPR - Winter 2018

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    Blog Post

    Published

    Dec. 18, 2018, 1:17 p.m.

    ...proteins for optogenetic control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor...

42. Bringing Sustainable Practices to the Lab: Easy Action Items

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    Blog Post

    Published

    April 22, 2019, 12:26 p.m.

    ...addition to doing some of the quick things above, try joining or starting a sustainability project. This could...

43. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type

    Blog Post

    Published

    Jan. 21, 2016, 4:52 p.m.

    ... specific subjects in the interview and happy listening! Download the podcast here! Podcast breakdown ...

44. 500,000 Plasmids Shipped and Counting!

    Type

    Blog Post

    Published

    March 26, 2015, 3:24 p.m.

    ... as shown in the graph below. From our humble beginnings in 2004 to today, you can see how plasmid distribution...

45. Fueled by Coffee at #SfN14

    Type

    Blog Post

    Published

    Nov. 18, 2014, 7:15 p.m.

    ... most of this great opportunity – so if you’re running out of energy, then it’s time for a coffee run!...

46. What's New in CRISPR - May 2019

    Type

    Blog Post

    Published

    May 28, 2019, 12:49 p.m.

    ...lab identified a Cas9 protein from Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide...

47. Inntags: Innovative Protein Epitope Tagging

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    Blog Post

    Published

    Nov. 10, 2015, 3:30 p.m.

    ...structure, inntags may become the tags of choice for examining protein-protein interactions, particularly in ...

48. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

    Type

    Blog Post

    Published

    June 26, 2014, 2:23 p.m.

    ...are usually unresolved and small, so I suggest designing your primer at least 50bp upstream of the sequence...

49. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type

    Blog Post

    Published

    July 26, 2018, 12:46 p.m.

    ...translocation rates are another variable to consider when designing KTR experiments. Third, KTRs are not well suited...

50. Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors

    Type

    Blog Post

    Published

    March 17, 2021, 2:40 p.m.

    ...pilates, jewelry making, reading, sailing, and gardening. Learn more about Julie Cicalese  Ms. Cicalese...