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  1. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...Cell/Science paper. You do need multiple first/corresponding author publications in quality journals. It ... of guidance or the document examples are not especially helpful. I set out to make something practical...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...likely it will contain random synthesis errors, especially single basepair deletions. Therefore, Gibson ...enzymes nick the DNA strand that contains their respective recognition site. This means that if the BbvCI...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...targeted nucleases In an effort to combine the respective advantages of the AAV and CRISPR-based approaches...efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers could apply this technique...

  5. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...as FSC-A/SSC-A, FSC-H/SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram...population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am free ...

  6. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...should ultimately contain radioactive bands that correspond to every nucleotide in your DNA sequence, based...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ...

  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ... and these are all symptoms of a negotiation, especially when money is involved. However, it doesn’t have...how much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... difficult to quantify accurately, but can be especially helpful to generate more signal from low abundance...the multicolor overlay; this helps all viewers, especially if you have some complex patterns with several...

  10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...recombinase expression may be replaced by sensors that correspond to the desired inputs. Such sensors do not necessarily...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...

  11. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...allows you to easily orient your gene insert with respect to the promoter. For example, let’s say your plasmid...being made (post or pre run in the table above, respectively). Some of the above stains require you to visualize...

  12. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ...important to test it in the brain region of interest, especially when using DIO/FLEx vectors for off-target expression...relatively low recombination rate (0.1% and 0.13% respectively) still required a 200-300 fold dilution to minimize...

  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ... lab Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware... my experience in the lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb ...

  14. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...considerations For changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher...efficiency, while for edits 3’ to the PAM sequences corresponding to the opposite strand were better (Farboud ...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...validation techniques for academia, industry, and especially the clinic. Popular validation assays are insufficient...search the whole genome for off-target sites irrespective of in silico prediction. These techniques differ...

  16. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... of a pathway to inhibit to block the cellular response to a particular ligand (such as when a component...pathway. For example, one could study the cellular response to a cytokine known to cause the up-regulation...

  18. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...through the excitation light path. Each hole corresponds to a different location in the sample. As more...step that reassigns out of focus light to its respective focal plane. In sufficiently thin samples, deconvolution...

  19. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...lower than the energy of the absorbed photon. Correspondingly, wavelengths of emitted photons are higher ...FRET).   Figure 1: Emission spectra and respective colors of a few commonly used fluorophores. Each...

  20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...mastered this technique so it might be worth a try, especially since the potential rewards could be substantial...) distance at which 50% FRET occurs. b Range corresponds to calculated D/A distances for observed FRET...
Showing: 261 - 280 of 545 results