We narrowed to 559 results for: ESP

...are many reasons why client situations might be especially challenging. For instance, the client might need...at business schools, and consulting clubs are especially helpful since they both provide up-to-date information...

...determine this is to examine expression of the early response gene c-fos. For example, using Chr2 stimulation...cue. The hippocampus has long been known to be responsible for mediating this behavioral effect but the ...

... the journals themselves should be primarily responsible for storage.  If datasets are too large to transfer...Improving access to data promotes dissemination, especially for young researchers who may be intimidated ...

...using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the pDS132 MCS ...bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard...

...lentiviruses lack a functioning integrase, the enzyme responsible for inserting viral DNA into the host genome....unwanted interruptions. Insertional mutagenesis is especially concerning in clinical settings, as evidenced...

...as FSC-A/SSC-A, FSC-H/SSC-H, or FSC-W/SSC-W, respectively. H, A, and W describe the shape of a histogram...population into DN, DP and SP (for each stain respectively). (B) Instead of a quadrant gate, I am free ...

...Cell/Science paper. You do need multiple first/corresponding author publications in quality journals. It ... of guidance or the document examples are not especially helpful. I set out to make something practical...

...targeted nucleases In an effort to combine the respective advantages of the AAV and CRISPR-based approaches...efficiencies in T-cells and hematopoietic stem cells respectively. Future researchers could apply this technique...

...necessary for post-translational modifications. Despite these potential issues, GFP has been used successfully...complement a knock-down or knock-out of the corresponding protein, that is strong evidence that the fusion...

...likely it will contain random synthesis errors, especially single basepair deletions. Therefore, Gibson ...enzymes nick the DNA strand that contains their respective recognition site. This means that if the BbvCI...

...should ultimately contain radioactive bands that correspond to every nucleotide in your DNA sequence, based...Figure 2). Accuracy and accessibility led to the widespread use of Sanger sequencing. Building upon this ...

... and these are all symptoms of a negotiation, especially when money is involved. However, it doesn’t have...how much do you need to start your lab”. You can respond by saying, “The exact start up costs may vary greatly...

...spatially and temporally control gene expression, especially in in vivo models, and new Cre-lox based technologies...included inducible Cre, such as the tamoxifen-responsive CreERT, the coupling of Cre-lox with FLP-FRT ...

...cardiac, and retinal cells (Issa et al., 2023). AAV2 Despite being isolated second, AAV2 is the best studied...expression, and can cross blood vessel barriers. It is especially popular for applications targeting liver tissue...

... difficult to quantify accurately, but can be especially helpful to generate more signal from low abundance...the multicolor overlay; this helps all viewers, especially if you have some complex patterns with several...

...recombinase expression may be replaced by sensors that correspond to the desired inputs. Such sensors do not necessarily...described in Nielsen et al (3) and Lou et al (4). Despite choosing proper register parts and designs, we ...

...allows you to easily orient your gene insert with respect to the promoter. For example, let’s say your plasmid...being made (post or pre run in the table above, respectively). Some of the above stains require you to visualize...

...important to test it in the brain region of interest, especially when using DIO/FLEx vectors for off-target expression...relatively low recombination rate (0.1% and 0.13% respectively) still required a 200-300 fold dilution to minimize...

... lab Undergraduate student Roles and responsibilities can vary "Lab assistants" usually wash glassware... my experience in the lab? Ask your questions—especially if it’s scary. I’ve been afraid to ask “dumb ...

...considerations For changes 5’ to the PAM, sequences corresponding to the same strand as the PAM produced a higher...efficiency, while for edits 3’ to the PAM sequences corresponding to the opposite strand were better (Farboud ...