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We narrowed to 517 results for: Emb

Showing: 261 - 280 of 517 results

  1. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  3. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...you give someone a card they are more likely to remember you if you follow up and if you give someone your... online to follow up with in person. Email lab members that have moved on to see if they will attend. ...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

  6. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    .... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

  7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  8. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

  10. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

  11. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...

  16. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

  17. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

  20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...TET, can, like the iron ball-and-chain that they resemble, actually impede your investigation into the regulation...CpG so that nothing else can interact with it. Remember, the goal of this experiment is to expose an unmethylated...
Showing: 261 - 280 of 517 results