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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 566 results for: FIE

Showing: 261 - 280 of 566 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...preexisting. This was previously debated in the cancer field and was difficult to determine using NGS because...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...

  3. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... few patterns throughout the project where it solidified some concepts that are heard often during undergrad...dreaded coming to work because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute...

  4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The...is incorporated into Nanoblades, just like the unmodified MLV Gag protein. After Gag-Cas9 is incorporated...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...synapse from one cell to another, it must replicate. Modified Rabies Virus: Converting a transsynaptic neuronal...first-order connections to the starter cell, RABV was modified to only infect user-defined starter cell types...

  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ... use in mice and human brain IHC and uploading verified antibodies onto the mAb3D atlas website. This ... into expression constructs to be conveniently modified and engineered.   Fig. 1: Antibody production...

  8. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...fluorescent protein, producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. ...fluorescence complementation toolkit from Benjamin Field’s lab. Special Considerations There are a few special...

  9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...During the 2003 SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV...expressing SARS-CoV-2 open reading frames and identified interactions of these proteins with human proteins...

  10. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...is to collaborate with specialists in different fields that can provide different insight into an area...your work and possibly a new perspective on the field. 3. Highlight your network and your collaborators...

  11. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...on or continue their careers in science-related fields. In particular, two recent events have the potential...Workshops will be run by experts in their respective fields: David Cameron, PhD - Director of Media Communications...

  12. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...into science communication are as varied as the field itself. Among other things, science communication...blog that I started writing three years ago on a field trip in Indonesia. Since then, I have tried many...

  13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...poster will naturally sit outside of the reader’s field of view because of its length, and will be more ...

  14. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...viral RNA is first converted to cDNA and then quantified using qPCR primers targeting specific viral components...with the antibiotic, and colony-forming units quantified. While these methods tend to be more accurate...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...anhydrous tetracycline. Three new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein...easier to deliver, but so far has few inhibitors identified.     New from the viral service By Jason Nasse...

  18. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    .... CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it ...eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...will depend on the virus type. AAV is typically purified using methods like ultracentrifugation, while ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...

  20. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a nuclease into three...make a precise edit or insertion via a chemical modifier. We used these categories to see what kinds of...
Showing: 261 - 280 of 566 results