We narrowed to 570 results for: FIE

...diversity in your gRNA library, but this should be verified by NGS before beginning your experiments. .... Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-virus-induced cell...

...processes. Every plasmid we receive is rigorously verified before becoming available to the community. This...overnight cultures with 15% glycerol), as opposed to purified DNA or colony sequencing. This means that we are...

...preexisting. This was previously debated in the cancer field and was difficult to determine using NGS because...barcodes in the population (all barcodes can be amplified using the same sets of forward and reverse primers...

...learning curve for the jargon and background in your field, but sometimes papers are also just poorly written... you are keeping up with the literature in your field of interest. Set aside some time each day or week...

...synapse from one cell to another, it must replicate. Modified Rabies Virus: Converting a transsynaptic neuronal...first-order connections to the starter cell, RABV was modified to only infect user-defined starter cell types...

...into science communication are as varied as the field itself. Among other things, science communication...blog that I started writing three years ago on a field trip in Indonesia. Since then, I have tried many...

... few patterns throughout the project where it solidified some concepts that are heard often during undergrad...dreaded coming to work because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute...

... use in mice and human brain IHC and uploading verified antibodies onto the mAb3D atlas website. This ... into expression constructs to be conveniently modified and engineered.   Fig. 1: Antibody production...

...fluorescent protein, producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. ...fluorescence complementation toolkit from Benjamin Field’s lab. Special Considerations There are a few special...

...During the 2003 SARS outbreak, Hyeryun Choe’s lab identified that ACE2 is the functional receptor for SARS-CoV...expressing SARS-CoV-2 open reading frames and identified interactions of these proteins with human proteins...

...to select for successfully modified zygotes or embryonic stem cells. Modified embryonic stem cells are ...

...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...poster will naturally sit outside of the reader’s field of view because of its length, and will be more ...

...bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9 fusion protein The...is incorporated into Nanoblades, just like the unmodified MLV Gag protein. After Gag-Cas9 is incorporated...

...is to collaborate with specialists in different fields that can provide different insight into an area...your work and possibly a new perspective on the field. 3. Highlight your network and your collaborators...

...on or continue their careers in science-related fields. In particular, two recent events have the potential...Workshops will be run by experts in their respective fields: David Cameron, PhD - Director of Media Communications...

...viral RNA is first converted to cDNA and then quantified using qPCR primers targeting specific viral components...with the antibiotic, and colony-forming units quantified. While these methods tend to be more accurate...

... Classification of stem cells Stem cells are classified into two broad types according to their source...Kotton Four Factors that Differentiate the Stem Cell Field Resources on Addgene.org Visit Our Stem Cell Page...

...will depend on the virus type. AAV is typically purified using methods like ultracentrifugation, while ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...

...plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a nuclease into three...make a precise edit or insertion via a chemical modifier. We used these categories to see what kinds of...

.... CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it ...eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the...