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Showing: 261 - 280 of 553 results

  2. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ... few patterns throughout the project where it solidified some concepts that are heard often during undergrad...dreaded coming to work because I truly enjoy the field I am in, the atmosphere, and the people.” My absolute...

  3. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...poster will naturally sit outside of the reader’s field of view because of its length, and will be more ...

  4. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...is to collaborate with specialists in different fields that can provide different insight into an area...your work and possibly a new perspective on the field. 3. Highlight your network and your collaborators...

  5. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ...into science communication are as varied as the field itself. Among other things, science communication...blog that I started writing three years ago on a field trip in Indonesia. Since then, I have tried many...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...fluorescent protein, producing fluorescent signal. Image modified from Feng et al. 2017 under CC-BY license. ...fluorescence complementation toolkit from Benjamin Field’s lab. Special Considerations There are a few special...

  7. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...on or continue their careers in science-related fields. In particular, two recent events have the potential...Workshops will be run by experts in their respective fields: David Cameron, PhD - Director of Media Communications...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...viral RNA is first converted to cDNA and then quantified using qPCR primers targeting specific viral components...with the antibiotic, and colony-forming units quantified. While these methods tend to be more accurate...

  9. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...will depend on the virus type. AAV is typically purified using methods like ultracentrifugation, while ... P0), infecting new Sf9 cells, and getting an amplified P2 virus. This P2 virus can be produced in a greater...

  10. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a nuclease into three...make a precise edit or insertion via a chemical modifier. We used these categories to see what kinds of...

  11. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...anhydrous tetracycline. Three new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein...easier to deliver, but so far has few inhibitors identified.     New from the viral service By Jason Nasse...

  13. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    Published
    Jan. 10, 2017, 3:37 p.m.
    .... CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering, as it ...eliminated (Fig. 1) (1, 3). The gRNA can be further modified by incorporation of an RNA hairpin, such as the...

  15. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...using a simple PNK reaction or can be purchased 5’-modified from a company. However, unlike an RNA guide, ...your favorite model system, but the genome-editing field is advancing rapidly and it wouldn’t be a surprise...

  16. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...our older plasmids may not have an entry in this field. If you’ve deposited a plasmid without a purpose...Backbone: The starting vector that the depositing lab modified to create their new plasmid.  The Addgene Vector...

  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ... protein, RNA, or DNA. Finally, once you have identified the best expression system, you can then choose...embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro transcribed gRNA are...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...developed using a stereomicroscope to capture large field-of-views (6). For more comprehensive reviews on ... alcohol and methyl salicylate which was later modified by others to produce Murray’s clear solution.  ...

  19. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission...
Showing: 261 - 280 of 553 results