We narrowed to 354 results for: HRE
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TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m....the boss. Harvard Business Review, January 2007. Three Rules for Managing a Successful Team. Naturejobs...
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Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m.... et al’s directed mutagenesis also produced the three separate timers shown in the table: Fast-FT, Medium-FT... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog PostPublishedDec. 1, 2016, 3:30 p.m....design your targeting vector such that a set of three LoxP sites flank the resistance gene and the targeted... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....two-photon microscopy to detect action potentials and subthreshold events, including miniature excitatory postsynaptic... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....directed evolution and screening experiment with three of the proteins that had shown magnetic responses... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m....classified CRISPR plasmids containing a nuclease into three functional groups: nucleases, modulators, and chimeric... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m.... system reviewed here. The AID system relies on three components. First, the protein of interest must ... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog PostPublishedSept. 6, 2016, 2:30 p.m....every cancerous cell. Even so, to pose the greatest threat, cancerous cells still need to successfully infiltrate... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m....cells with AAV packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap... -
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity
TypeBlog PostPublishedOct. 11, 2018, 12:30 p.m....Gate Assembly test systems Our research focused on three different levels of assembly: High efficiency and... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....traced the 3-D path of the protein’s backbone in three-dimensional space, but their flexible and jiggly... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....number of hits to one sequence/gene - we set our threshold at >100 hits - immediately raises a red flag. ... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....not while you’re still in the thick of it. About three or four years into my PhD in Molecular Biology, ... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....Nature Methods, 4, 331–336. Pubmed. P. Keller, M.B. Ahrens. (2015) Neuron, 85, 462-483. Pubmed. E.H.K. Stelzer... -
Which Fluorescent Protein Should I Use?
TypeBlog PostPublishedMay 20, 2014, 2:06 p.m.... Plasmids 101: Green Fluorescent Protein (GFP) Three Review Articles on the Different Types of FPs: ... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....about how to balance those roles. I’m a father of three, a scientist and science communicator, and I strive... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....data was published. Fig. 1: Testing of three different AAV serotypes (AAV1, 8, 9) injected into... -
AAVs in Retinal Gene Therapy
TypeBlog PostPublishedJuly 31, 2018, 12:55 p.m....another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular dystrophy... -
Plasmids 101: Secondary Nanobody Toolbox
TypeBlog PostPublishedFeb. 27, 2018, 2:04 p.m....colocalization is possible even when staining cells with three IgG1 antibodies. Antibodies were individually incubated... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....AAV2) – or some unprecedented combination of the three (Servellita et al., 2023). Normally when children...