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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 316 results for: Mut

Showing: 261 - 280 of 316 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

  2. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

  3. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

  4. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...

  7. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due...

  8. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

  9. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...

  15. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...column runs. The TEV protease itself, with various mutations used to increase its stability activity, can be...

  16. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...the researcher, Zeocin has been shown to cause mutations even in cells with the resistance gene, so it ...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ...

  18. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...between cells lines, as this can be dependent on the mutation rate of the cell line, but it is broadly recommended...

  20. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...sometimes (but not always) be noted in in the “mutation” field on the plasmid page (see plasmid 87904 ...
Showing: 261 - 280 of 316 results