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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 548 results for: PPL

Showing: 261 - 280 of 548 results

  1. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...latest developments in genetics research, their applications and regulatory policies; keeping tabs on the...and who will be affected by the knowledge and applications generated from this research. At both a practical...

  4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...potential to be used for other types of CRISPR applications. Let’s take a look at the key components of ...After Gag-Cas9 is incorporated, the MLV protease, supplied as part of the MLV Gag-Pol polyprotein, cleaves...

  5. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...that can be used to simulate muscle tissue as it applies to C. elegans locomotion. To see the other resources...biology in C. elegans." Nucleic acids research 36.suppl_1 (2007): D57-D62. PubMed PMID: 17986455. PubMed ...

  6. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things in biology are...phenotype is best described with “it’s complicated.” Applying MoClo Interested in using MoClo? You don’t have...

  7. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  8. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...focused on developing cell-free synthetic biology applications for human health and the environment.     Alexander...nanomaterials science, and self-assembly for applications in health, energy, and sustainability.   References...

  9. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...eclipse any of the CRISPR nucleases in AAV-based applications. Get More Cpf1 Background Here THE ORIGINAL ...the PAM sequence. Many research and clinical applications require HDR over NHEJ, so comparing HDR efficiency...

  10. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...the initial laser output. The same considerations apply to the polishing and preparation of the patch cable...Injectable, cellular-scale optoelectronics with applications for wireless optogenetics. Science. 2013;340...

  11. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives...contributes each month - with only occasional supplementation with grant funds, money is always tight. Of...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...and respond to novel substances. We both had an applied science bent, so the engineering aspect of this...about continuing with the business. We ended up applying to a business accelerator freely stating that ...

  13. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...something you may have to optimize for your specific application. With mammalian systems, it is standard to do...titer the virus, or go straight into your viral application, like protein production. Titration of baculovirus...

  14. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...machine learning (PRIDICT, PRIDICT2.0).   How to apply PRIDICT in prime editing experiments Prediction ... computational biology. His expertise includes applying machine learning techniques to make sense of biology...

  15. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment! ...Scarless Modification of Proteobacterial Genomes. Applied and Environmental Microbiology, 85(16). https:/...

  17. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...been excitedly testing NgAgo's genome editing applications. So, how does NgAgo stack up thus far? A few... S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell...

  19. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...species in question), why not use them whenever applicable?Against this, consider the cost of an accidental...An accidental release could delay real-world applications against scourges such as malaria and dengue ...

  20. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ... critical for a number of popular scientific applications, and the quality of a standard curve can make...for an acceptable R2 but for many scientific applications users aim for an R2 above 0.95.    Additional...
Showing: 261 - 280 of 548 results