We narrowed to 290 results for: Pol

1. Overcoming the AAV Size Limitation for CRISPR Delivery

   Type

   Blog Post

   Published

   Sept. 16, 2020, 1 p.m.

   ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...

4. Transferable Skills Guide: External Collaborations

   Type

   Blog Post

   Published

   April 19, 2018, 1:25 p.m.

   ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

5. AAV Titers: Where Do They Come From And What Do They Mean?

   Type

   Blog Post

   Published

   Nov. 15, 2017, 3 p.m.

   ...Paolo, et al. "Systemic errors in quantitative polymerase chain reaction titration of self-complementary...

6. Career Insights: Technical Support Specialist

   Type

   Blog Post

   Published

   Nov. 9, 2017, 2 p.m.

   ...the job. When dealing with customers, you must be polite, friendly, patient, attentive, empathetic, and ...

7. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ... Marc Sze, Victoria McGovern, and Julie Wolf. Apologies to anyone I’ve inadvertently left off this list...

8. Plasmids 101: Broad Host Range Plasmids

   Type

   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...

9. The Challenges of Cell Culture

   Type

   Blog Post

   Published

   May 5, 2016, 2:30 p.m.

   ...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...

10. Plasmids 101: E. coli Strains for Protein Expression

    Type

    Blog Post

    Published

    Feb. 10, 2015, 3:06 p.m.

    ...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...

11. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...mitochondrial matrix (Rhee et al., 2013) and determine the topology of the mitochondrial calcium uniporter (Martell...

12. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

13. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...

14. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...

15. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...

16. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...

17. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

18. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

19. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

20. Antibodies 101: Multiplex Immunofluorescence

    Type

    Blog Post

    Published

    May 23, 2023, 1:15 p.m.

    ... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG   If you need more...