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We narrowed to 285 results for: Pol

Showing: 261 - 280 of 285 results

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CRISPR 101: Targeting RNA with Cas13a (C2c2)

Type

Blog Post

Published

Sept. 21, 2017, 2:07 p.m.

...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

Published

April 11, 2024, 1:15 p.m.

...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

Published

Oct. 13, 2022, 1:45 p.m.

...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...
The Challenges of Cell Culture

Type

Blog Post

Published

May 5, 2016, 2:30 p.m.

...whether the cell lines had isoenzymes and genetic polymorphisms specific to their origins. This technique is...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

Published

Feb. 10, 2015, 3:06 p.m.

...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

Published

Dec. 2, 2019, 2 p.m.

...mitochondrial matrix (Rhee et al., 2013) and determine the topology of the mitochondrial calcium uniporter (Martell...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

Published

Aug. 15, 2019, 12:30 p.m.

...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Type

Blog Post

Published

April 9, 2019, 1:13 p.m.

...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...
Antibodies 101: Introduction to Immunofluorescence

Type

Blog Post

Published

Nov. 16, 2021, 2:15 p.m.

...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...
Antibodies 101: Single Chain Fragment Variables (scFvs)

Type

Blog Post

Published

June 3, 2021, 1:15 p.m.

...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...
Adeno-associated Viruses (AAVs) for Genome Editing

Type

Blog Post

Published

March 27, 2018, 1:32 p.m.

...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...
Multiple Plasmids at a Low Price: Kits from Addgene

Type

Blog Post

Published

Sept. 14, 2016, 2:30 p.m.

...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

Published

June 23, 2016, 2:30 p.m.

...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

Type

Blog Post

Published

May 10, 2016, 2:30 p.m.

...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

Published

June 24, 2020, 5:45 p.m.

...https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...
Is this the right place for me? 8 tactics for choosing a lab

Type

Blog Post

Published

Oct. 2, 2018, 12:56 p.m.

...treat one another (especially those low on the totem pole). Can you see yourself enjoying this atmosphere?...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

Published

May 23, 2023, 1:15 p.m.

... Kv2.1 channel anti-Kv2.1 KC (Rabbit IgG polyclonal) Goat anti-Rabbit IgG If you need more...
Finding Your Perfect Job After University

Type

Blog Post

Published

Jan. 12, 2016, 3:30 p.m.

...and non-profit) you can also get a lot of office politics (many individuals trying to divert more resources...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

Published

Oct. 8, 2020, 1:15 p.m.

... Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction. In: Methods in Molecular Biology...
A History of Genome Engineering in Popular Culture

Type

Blog Post

Published

Feb. 25, 2020, 2:15 p.m.

...what would happen if these corporations would extrapolate genetic engineering to exploit human beings. ...

Showing: 261 - 280 of 285 results

Results per page:

CRISPR 101: Targeting RNA with Cas13a (C2c2)

Plasmids 101: Broad Host Range Plasmids

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

The Challenges of Cell Culture

Plasmids 101: E. coli Strains for Protein Expression

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Harnessing Bacterial Toxins for Allelic Exchange

Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

Antibodies 101: Introduction to Immunofluorescence

Antibodies 101: Single Chain Fragment Variables (scFvs)

Adeno-associated Viruses (AAVs) for Genome Editing

Multiple Plasmids at a Low Price: Kits from Addgene

Using Phosphoserine to Study Protein Phosphorylation

Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

CRISPR 101: Epigenetics and Editing the Epigenome

Is this the right place for me? 8 tactics for choosing a lab

Antibodies 101: Multiplex Immunofluorescence

Finding Your Perfect Job After University

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

A History of Genome Engineering in Popular Culture

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