We narrowed to 289 results for: Pol

1. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

   Type

   Blog Post

   Published

   Aug. 13, 2019, 12:58 p.m.

   ..., including investigations into environmental pollutants and health, embryo growth, brain function, and...

2. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

   Type

   Blog Post

   Published

   April 2, 2019, 1:44 p.m.

   ...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...

3. Lab to Office Culture Shock

   Type

   Blog Post

   Published

   Feb. 5, 2019, 1:04 p.m.

   ...of the causes of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list...

4. Plasmids 101: Stringent Regulation of Replication

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   Blog Post

   Published

   June 20, 2024, 1:15 p.m.

   ... from the ori. In the elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication...

5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

   Type

   Blog Post

   Published

   Oct. 13, 2022, 1:45 p.m.

   ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

6. How to Be an Excellent Trainee

   Type

   Blog Post

   Published

   Sept. 26, 2023, 1:15 p.m.

   ...It’s often easiest to train individuals who are polite, appreciative, and clear about their needs. Remember...

7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ... the cells could be batch-sorted to isolate a polyclonal cell line with strong gas vesicle expression ...

8. Plasmids 101: Broad Host Range Plasmids

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   Blog Post

   Published

   April 11, 2024, 1:15 p.m.

   ...structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts. In a plasmid...

9. Plasmids 101: E. coli Strains for Protein Expression

   Type

   Blog Post

   Published

   Feb. 10, 2015, 3:06 p.m.

   ...induction system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control...

10. Adeno-associated Viruses (AAVs) for Genome Editing

    Type

    Blog Post

    Published

    March 27, 2018, 1:32 p.m.

    ...choice. These vectors are very similar, both with polylinkers for the addition of gene-specific 1 kb homology...

11. Multiple Plasmids at a Low Price: Kits from Addgene

    Type

    Blog Post

    Published

    Sept. 14, 2016, 2:30 p.m.

    ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...

12. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...

13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type

    Blog Post

    Published

    May 10, 2016, 2:30 p.m.

    ...Addgene. Kaminski R, Chen Y, Fischer T, Tedaldi E, Napoli A, Zhang Y, Karn J, Hu W, Khalili K. Elimination...

14. Harnessing Bacterial Toxins for Allelic Exchange

    Type

    Blog Post

    Published

    Aug. 15, 2019, 12:30 p.m.

    ...tuner: a computer program for the construction of polylinker sequences of molecular vectors". Proceedings ...

15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type

    Blog Post

    Published

    April 9, 2019, 1:13 p.m.

    ...Bendezú, Felipe O., et al. "Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based...

16. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...

17. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and...

18. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...mitochondrial matrix (Rhee et al., 2013) and determine the topology of the mitochondrial calcium uniporter (Martell...

19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type

    Blog Post

    Published

    June 24, 2020, 5:45 p.m.

    ...https://doi.org/10.1093/nar/gky1286 Hilton IB, D’Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy...

20. Finding Your Perfect Job After University

    Type

    Blog Post

    Published

    Jan. 12, 2016, 3:30 p.m.

    ...and non-profit) you can also get a lot of office politics (many individuals trying to divert more resources...