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We narrowed to 610 results for: REV

Showing: 261 - 280 of 610 results

  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...context, it’s hypothesized that base removal helps prevent the invading genome from “recovering” its original...cannot switch guides. While this fidelity would prevent other small nuclear DNAs from binding NgAgo, it...

  5. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... need to get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed...new technologies that will bring about the next revolution in biological research. We’re incredibly happy...

  6. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...Genetics and Hereditary Symbiosis" Physiological Reviews 32.4 (1952) 403-430. Pubmed PMID: 13003535. Lederberg...Bacterial Plasmids: A Proposal.”Bacteriological Reviews 40.1 (1976): 168–189. PubMed PMID: 16350226. PubMed...

  7. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...obtained cells from patients with the condition to reveal some “outstanding differences.” They now have some... they now think that mutations in the gene may prevent neurons from returning to basal conditions normally...

  8. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ... decision before making it, to have an internal review system.  On top of these benefits, potential funding...Addgene blog Read our recent post on Synbio start up Revbio  ...

  9. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...multiple selectable markers. Bob Goldstein’s lab previously used a transgene selection strategy for their...represents another leap forward for the CRISPR/Cas9 revolution!   References Streamlined Genome Engineering...

  11. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...high-throughput next generation (NGS) sequencing have prevented laboratories from using these methods for routine...inserts and larger targets. The NGS readout also reveals additional information about the presence of non-plasmid...

  12. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...therapeutics for complex genetic diseases. So we’re revisiting this post to look at CRISPR’s potential for use...Technology and its Application in Genetic Diseases: A Review.” Current pharmaceutical biotechnology. https:/...

  13. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...includes a step that both fragments the DNA and prevents it from forming concatemers later. The "FS" stands..., one was only able to extract libraries from previously fragmented DNA.The general process for library...

  14. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...for visualizing genomic loci in living cells. Previous iterations of genomic loci visualization tools...CRISPR-Sirius (Ma et al., 2018). They improved on previous CRISPR-based loci visualization tools by increasing...

  15. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    Published
    Nov. 7, 2019, 2:18 p.m.
    ...studies, and has held various editorial roles on review and primary research journals including Trends ...who produce a broad range of primary research and review journals across life and physical science, and ...

  16. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...day in advance. This gives everyone a chance to review the agenda and add anything that is missing.  During...Send around relevant documents, like proposals to review or project plans, before the meeting. At Addgene...

  17. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...brightness, photostability, and so on; see the previous blog post for more discussion of these factors...that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...quantify them with biosensors! In this blog we will review the basics of biosensors and how to use viruses...building blocks. Physiological factors Unlike the previously described sensors, this class measures either...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV...
Showing: 261 - 280 of 610 results