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  1. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance ...

  2. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... is fast and can cut in as little as 2 hours.  Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org...Expanded serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes...

  4. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...landscape facing all scientists, both young and established, as they embark on or continue their careers .... and Social Media Content Specialist at Thermo Fisher Scientific Developing effective science advocates...

  5. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...reels in the non-target strand of DNA much like a fisherman would reel in a big catch. It was originally suspected... Alyssa Shepard...

  7. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin... Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent regulation...

  9. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

  10. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

  11. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ...

  12. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...well as premixed and ready-to-use reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good...you. Happy cloning (and be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun...

  13. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...temperature for mammalian cells and one which diminishes the endonuclease activity of the enzyme. In vivo...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received...

  15. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

  16. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...section. The GFP story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was not...produced by the quieter, more reserved Franklins and Prashers will find it increasingly difficult to compete...

  17. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...structure and could also cause your target to be washed away. Furthermore, these chemicals can also alter...: Always always always look at your antibody datasheet to see if it has been tested in IF and if a protocol...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ...

  20. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...Small’ data produced by the research group in spreadsheets, Word docs, and the like is likely stored in... documents, but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or ...
Showing: 261 - 280 of 512 results