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We narrowed to 385 results for: abo.5

Showing: 261 - 280 of 385 results

  1. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ... 83896 AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... via focused ultrasound. Nat Biomed Eng. 2021 Nov;5(11):1336-1347. doi: 10.1038/s41551-021-00779-w. Epub... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    .... Cells should die within 3-5 days and resistant colonies appear in about 10-14 days depending on how ...

  4. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB Jr, Pewe L, Wohlford-Lenane C, Hickey M,...the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to share plasmids... availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley...mouse strain is regaining popularity. The Jackson Laboratory, a nonprofit research institution, has been able...clinical manifestations, transmissibility, and laboratory studies of COVID-19: an observational study. ...

  5. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...found that KillerRed required longer exposure times (5 minutes vs 10 seconds) to achieve the same level of... in culture and in vivo.  Read on to learn more about this killer illumination technique! What is a photosensitizer...within the near-infrared (NIR) spectrum (>620 nm). Above is a brief schematic of how the MG-2I-dL5** FAP-...use MG-2I-dL5** with your research? Let us know about your experiences using photosensitizers in the comments... to Receive the Fluorescent Protein eBook Learn about Small Molecule Fluorophores Resources on Addgene.org...

  6. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ... vectors through pseudotyping.” Curr. Gene. Ther. 5 (2005) 387-398. PubMed PMID: 16101513. PubMed Central...applications to help you start (safely!) thinking about the possibilities. Ready to plan your next (or maybe...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed...Additional Resources on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy...Addgene.org Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors...

  7. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    Published
    Feb. 24, 2015, 7:50 p.m.
    ...Ishitani R, Zhang F, Nureki O. Cell. 2014 Feb 27;156(5):935-49. doi: 10.1016/j.cell.2014.02.001. Epub 2014...that screen with a system you are really confident about working for most genes and individual targeting ... system, Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could...RNA we use. What we have to do now is learn more about the rules.” He said that there is no theoretical...References & Further Reading: For more information about SAM, including a tool for getting optimized guide...

  8. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...insert. If the insert is large, we may only check the 5’ and 3’ ends to verify that the sequence assembly ...One of the best things about sharing plasmids through Addgene is that we provide an added level of confidence...bp of clean sequence from a typical reaction, or about 250 amino acids worth, so make sure the feature ...96-well microplates (identical copies, see image above). We then select forward and reverse primers for...specifically to target this region, as outlined above. Avoid primers that bind within or must read through...

  9. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Retroviruses and cancer. Rev Infect Dis. 1982 Sep-Oct;4(5):1015-25. DOI: 10.1093/clinids/4.5.1015 Lee C.S., ...clinical settings. AAV is also a cool tool in the laboratory! It is ideal for whole animal administration,... be helpful to you. Interested in learning more about a specific virus? We have you covered with guides..., adenovirus, and AAV. If you’re wondering more about what applications these viruses are good for, we...

  10. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...will end up with a stellar letter of support.        5. Application submission  Set a calendar reminder for...Rose is passionate about outreach among low-income communities to educate parents about resources that can...automated filters of consideration. If you are worried about any eligibility provision, don’t guess. Simply reach...

  11. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...degradation. Nature Chemical Biology. May 2018. 14(5): 431-441. PubMed PMID: 29581585. Additional resources...which can be used with this approach - read more about them on our CRISPRa blog! Fig. 1 CRISPR ... is a lot of overlap with the systems discussed above, just with more elements of control. Tet promoters...to be site-specifically knocked in. This can be labor intensive and time consuming. If your native protein...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...PMID: 25775042. PubMed Central PMCID: PMC4414919. 5. Yarmolinsky, Michael B., and L. Gabriel. "Inhibition... FUNCAT Professor Schuman’s technique applies metabolic labeling using a noncanonical amino acid, azidohomoalanine...both quantitative and spatio-temporal information about the turnover of specific endogenous proteins in ... interested in neurobiology.  She is passionate about communicating science to all audiences. Right now...

  13. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...for your species. Affinity purification Only up to 5% of the IgG antibodies produced by an animal are specific...  Read about another common type of antibody for research, monoclonal antibodies Learn about the advantages...Once the initial antibody response starts to wane about a month later, the animal is given additional booster...

  14. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    Published
    Sept. 4, 2018, 12:53 p.m.
    ...phase, the number of plasmids per cell increases 4-5 fold (for pUC plasmids) or ~2 fold (for p15a, R6K,...is useful for many metabolic engineering and synthetic biology goals. In metabolic engineering, the expression...or can interfere with the precision needed in metabolic engineering or other synthetic biology circuits...

  15. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...tropism and axonal transport of AAV serotypes 1, 2, 5, 6, 8 and 9 in the mouse brain. PLoS One. 2013 Sep...Resources on the Addgene Blog Learn More About Lentivirus Learn more About AAV Get the Scoop on Our Viral Service...

  16. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ...mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes, such ... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here.    ...tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express various fluorescent...

  17. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ... are safe! Colony screening Pick a few colonies (5-10) and grow them in a small culture volume (2 or ...they get old, they lose competency). If all the above is correct, you should probably have a second and...blog posts Read blog posts on plasmid cloning Read about different plasmid elements Resources on Addgene.org...

  18. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...were never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out....return the sample back to the original pH. What about putting it back in the freezer? We then tested whether...

  19. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...CC-BY-NC-ND 4.0 International license. Figure 5: A) Simultaneous fluorescence (upper trace) and electrophysiological... view of traces corresponding to the dashed box above. The number of action potential (AP) spiking events...

  20. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is no requirement, which...you are a structure junky who just wants to learn about Cas domains. CasPEDIA either directly has this information...
Showing: 261 - 280 of 385 results