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We narrowed to 816 results for: nin

Showing: 261 - 280 of 816 results

  1. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ...great experimental plan that requires indirect staining of both targets in your specimen. You scour the...Why does isotype matter? Isotype matters when constraining using the indirect method since a secondary ...alternative isotype (purple, mouse). Depending on the cloning process, all constant regions, just the heavy chain...isotype can not be used for multiplexed indirect staining methods since the secondary antibody used will...specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy chain constant...

  2. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    .... My eye was caught by a couple of unusual job openings, although they seemed too far afield at first ...forget: what would it be like? Then I spotted an opening for being an editor at Science magazine. That one...referees. And I am constantly teaching – often explaining a technical piece of research to others outside...of the world’s scientific discoveries into an entertaining podcast? Look at our multimedia producer and...Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...

  3. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...halves (Fig. 1): one containing the microtubule binding domain and the second containing the growth promoting...number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches used in the...developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...complexes are more efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that the ACE ...

  4. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... 101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...genomic locations. 6. CRISPR 101: Non-homologous End Joining So you know that CRISPR can be used to generate...gymnastics behind DNA repair by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why ...

  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation)...PALM, also FPALM) (11). For PALM, a small area containing inactive PA-FPs is photoactivated, imaged, and...and new variants are still being engineered. By combining PA-FPS with superresolution microscopy, scientists... Michelle Cronin...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1..., 1978), a measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other...due to tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically...labeling contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which...PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding the chemistry...

  7. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...to wonder how he has survived this long without downing any dihydrogen monoxide (H2O). Studying science...the same abstract but with the names reversed, meaning that the findings of the study were also reversed...were exhibiting a phenomenon called “motivated reasoning,” the catchall term for a range of neat little...nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin, 108(3), 480. More resources...

  8. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...produce custom gRNA-containing lentivirus, we are providing lentiviral preps containing highly requested ... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...expertise as we’ve gotten these services up and running. Both Alex Chavez and John Doench are members of...

  9. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...diversity is impressive, and researchers are just beginning to tap the wealth of CRISPR possibilities. To ... are described below. Yan et al.: WYL-domain-containing accessory protein WYL1 increases RspCas13d and...EsCas13d activity, indicating that WYL domain-containing proteins may be broader regulators of Cas13d ...Cas13d enzymes is another win for bioinformatic data mining. As we continue to harness the natural diversity...Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein.” Mol Cell. (2018) pii: S1097...

  10. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein containing Reverse...example: pLXSN has the wild type LTR, an MCS for cloning X gene, an SV40 promoter, and Neomycin selection...either side of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts... The transcript begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the...

  11. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility for ...student using viral vectors for the first time and learning how to purify AAV.  Second, you’ll identify your...captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40% layer. To collect...insert a syringe at the interface of the 40% layer containing the virus and 60% iodixanol layer, with the bevel...alt text in the comments! We learned a lot from listening to others' alt text descriptions, and we’d love...

  12. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...required components are very similar. While plasmids containing virus-specific elements such as the LTRs or structural...allows it to replicate episomally, essentially remaining an extrachromosomal part of the host genome that...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response...which ones could be helpful to you. Interested in learning more about a specific virus? We have you covered...

  13. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...at my company was open to meeting with me and explaining more of her role, which eventually led me to ... problems that they are trying to solve. After learning more about the stakeholders, the product managers...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...audience. Additionally, in order to keep everything running smoothly, you’ll want to be really organized. Often... hours ahead, so I’m always catching up in the morning! 9:00 AM--Technical implementation review. The ...

  14. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ... can be easily maintained when grown on media containing the missing nutrient. Scientists can exploit ...the host’s auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless ...weak. yes   LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen...used in gene disruption experiments. MET15 L-methionine and overproduces hydrosulfide ions yes - Grow... L-leucine   S. pombe  no no   ade6+ purine (adenine)   S. pombe  no no   Considerations when using...

  15. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... labeling using a noncanonical amino acid, azidohomoalanine (AHA). In the technique, AHA is incorporated...incorporated into newly synthesized proteins instead of methionine. The labeled proteins can be detected using click-chemistry...click-chemistry that covalently links the azido containing AHA to a fluorescent alkyne tag. The newly synthesized...determine the turnover of a specific protein by combining it with the proximity ligation assay (PLA). The...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...Addgene’s 20th anniversary on Twitter. In the beginning Let’s take a quick flash back to 2004. “Shrek ...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant... make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a ...requestors alike. We transformed this process by aligning institutions with standardized agreements and ...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

  17. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...audience’s mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort...others. But sometimes we’re unaware that we are leaning on an unspoken expectation of our audience knowing...concept to another which leads us to… Framework Learning to think like an immunologist, a geneticist, a...needed to remove a distraction, such as pausing or turning off the slide deck (or reminding them to put their...

  18. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...hydropersulfide that is then transferred to thiol-containing acceptors. These hydropersulfides modulate protein... biosensor can also be used along other thiol-containing acceptors in competition assays. “I believe that...photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows a favorable outcome are then... to engineer many types of biosensors.   This screening method allowed Tian to optimize a red‑shifted ...neural microcircuits. “In the longer term, this screening platform may help us to create high-performance...

  19. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...How Non-Homologous End Joining Repairs Double Strand Breaks Get Tips on Designing Your gRNA Browse Our ...RuvC groove, Slaymaker et al. made a variety of alanine substitutions throughout the groove in 32 separate...diminish off-target cutting (4). However, instead of weakening interaction sbetween the non-target strand and... in applied and/or therapeutic settings. Will combining the different mutations found in each of these...

  20. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...produced in E1-containing mammalian cell lines such as HEK293 cells. 1st generation AdV cloning capacity is.... 2nd Gen AdVs were designed to have increased cloning capacity, decreased  potential for the generation...deleted in 2nd generation AdVs, thereby increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system... interest for gene therapy due their increased cloning capacity (up to 36 kb!), long-term transgene expression...
Showing: 261 - 280 of 816 results