We narrowed to 821 results for: nin

...Horvath P, Moineu S, Mojica FJM, Wolf YI, Yakunin JvdO, Koonin EV. Nature Reviews Microbiology. 2011 Jun...powerful and adaptive defense strategy against life-threatening viruses. Evolving to survive Although the CRISPR...CRISPR/Cas adaptive immune system seems like the winning ticket to ensure prokaryotic survival; however,...Cong et al. and Mali et al. in January 2013 (7, 8). Nine months later, more than 1500 publications describe...

...halves (Fig. 1): one containing the microtubule binding domain and the second containing the growth promoting...number of fluorophores. You can find plasmids containing the cobalamin binding riboswitches used in the...developing a new imaging-based bacterial colony screening platform, Oliver Griesbeck’s lab set out to engineer...complexes are more efficient than the BE3 plasmid containing rat APOBEC1. Finally, they show that the ACE ...

.... My eye was caught by a couple of unusual job openings, although they seemed too far afield at first ...forget: what would it be like? Then I spotted an opening for being an editor at Science magazine. That one...referees. And I am constantly teaching – often explaining a technical piece of research to others outside...of the world’s scientific discoveries into an entertaining podcast? Look at our multimedia producer and...Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...

...great experimental plan that requires indirect staining of both targets in your specimen. You scour the...Why does isotype matter? Isotype matters when constraining using the indirect method since a secondary ...alternative isotype (purple, mouse). Depending on the cloning process, all constant regions, just the heavy chain...isotype can not be used for multiplexed indirect staining methods since the secondary antibody used will...specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy chain constant...

...website in search of plasmids. Now, scientists are beginning to see Addgene as a large data set. Addgene has...database. Through a series of simulations and machine learning, they created a classifier that predicts whether...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...the genes deposited at Addgene were synthetic. But nine years later, over 20% of the genes deposited that...Nielsen, Alec AK, and Christopher A. Voigt. "Deep learning to predict the lab-of-origin of engineered DNA...

... 101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments and ...Addgenie Jessica Welch explains how Blue-White screening could be a potential solution. 5. Cpf1: A New ...genomic locations. 6. CRISPR 101: Non-homologous End Joining So you know that CRISPR can be used to generate...gymnastics behind DNA repair by non-homologous end joining (NHEJ). 7. Career Coaching for Scientists: Why ...

...for p-azido-l-phenylalanine E. coli pEVOL-pBpF synthetase for p-benzoyl-l-phenylalanine E. coli pAcBac1..., 1978), a measurement that is unaffected by intervening proteins, lipid bilayers, organelles or other...due to tens of thousands of biomolecules each containing multiple binding crevices that can nonspecifically...labeling contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which...PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding the chemistry...

...Jellyfish (Aequorea victoria) GFP (wtGFP) by mutating Threonine 203 to Histidine (T203H) (1). wtGFP normally contains...performing several rounds of random mutagenesis screening for enhanced RFP variants. PA-mCherry (E26V / ...naturally occurring Dronpa protein (named after dron, a ninja term for vanishing, and pa, for photoactivation)...PALM, also FPALM) (11). For PALM, a small area containing inactive PA-FPs is photoactivated, imaged, and...and new variants are still being engineered. By combining PA-FPS with superresolution microscopy, scientists... Michelle Cronin...

...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...to wonder how he has survived this long without downing any dihydrogen monoxide (H2O). Studying science...the same abstract but with the names reversed, meaning that the findings of the study were also reversed...were exhibiting a phenomenon called “motivated reasoning,” the catchall term for a range of neat little...nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin, 108(3), 480. More resources...

...produce custom gRNA-containing lentivirus, we are providing lentiviral preps containing highly requested ... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...expertise as we’ve gotten these services up and running. Both Alex Chavez and John Doench are members of...

...diversity is impressive, and researchers are just beginning to tap the wealth of CRISPR possibilities. To ... are described below. Yan et al.: WYL-domain-containing accessory protein WYL1 increases RspCas13d and...EsCas13d activity, indicating that WYL domain-containing proteins may be broader regulators of Cas13d ...Cas13d enzymes is another win for bioinformatic data mining. As we continue to harness the natural diversity...Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein.” Mol Cell. (2018) pii: S1097...

...at my company was open to meeting with me and explaining more of her role, which eventually led me to ... problems that they are trying to solve. After learning more about the stakeholders, the product managers...next-gen sequencing (NGS), and high-throughput screening. While some developers had science backgrounds...audience. Additionally, in order to keep everything running smoothly, you’ll want to be really organized. Often... hours ahead, so I’m always catching up in the morning! 9:00 AM--Technical implementation review. The ...

...years. But learn we did! From Addgene's very beginning, we had to consider digital accessibility for ...student using viral vectors for the first time and learning how to purify AAV.  Second, you’ll identify your...captured at the 17%, 25%, and 60% layers, and genome-containing virions are captured at the 40% layer. To collect...insert a syringe at the interface of the 40% layer containing the virus and 60% iodixanol layer, with the bevel...alt text in the comments! We learned a lot from listening to others' alt text descriptions, and we’d love...

...particle containing Matrix, Capsid, and Nucleocapsid components. Pol Precursor protein containing Reverse...example: pLXSN has the wild type LTR, an MCS for cloning X gene, an SV40 promoter, and Neomycin selection...either side of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5 kb, but inserts... The transcript begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the...

...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...required components are very similar. While plasmids containing virus-specific elements such as the LTRs or structural...allows it to replicate episomally, essentially remaining an extrachromosomal part of the host genome that...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response...which ones could be helpful to you. Interested in learning more about a specific virus? We have you covered...

... can be easily maintained when grown on media containing the missing nutrient. Scientists can exploit ...the host’s auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless ...weak. yes   LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen...used in gene disruption experiments. MET15 L-methionine and overproduces hydrosulfide ions yes - Grow... L-leucine   S. pombe  no no   ade6+ purine (adenine)   S. pombe  no no   Considerations when using...

... labeling using a noncanonical amino acid, azidohomoalanine (AHA). In the technique, AHA is incorporated...incorporated into newly synthesized proteins instead of methionine. The labeled proteins can be detected using click-chemistry...click-chemistry that covalently links the azido containing AHA to a fluorescent alkyne tag. The newly synthesized...determine the turnover of a specific protein by combining it with the proximity ligation assay (PLA). The...concerts and bake. you can contact her at rojas@ti2learning.org. References 1. Dieterich, Daniela C., et al...

...audience’s mental bandwidth.  Jargon Learning, using, and retaining new vocabulary takes time and effort...others. But sometimes we’re unaware that we are leaning on an unspoken expectation of our audience knowing...concept to another which leads us to… Framework Learning to think like an immunologist, a geneticist, a...needed to remove a distraction, such as pausing or turning off the slide deck (or reminding them to put their...

...hydropersulfide that is then transferred to thiol-containing acceptors. These hydropersulfides modulate protein... biosensor can also be used along other thiol-containing acceptors in competition assays. “I believe that...photoconvertible fluorescent protein mEOS4a. Cells containing a sensor that shows a favorable outcome are then... to engineer many types of biosensors.   This screening method allowed Tian to optimize a red‑shifted ...neural microcircuits. “In the longer term, this screening platform may help us to create high-performance...

...Addgene’s 20th anniversary on Twitter. In the beginning Let’s take a quick flash back to 2004. “Shrek ...business practices, like accepting e-signatures and designing a website users could easily navigate. This meant... make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical School. We want to give a ...requestors alike. We transformed this process by aligning institutions with standardized agreements and ...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...