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Showing: 261 - 280 of 694 results

  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...obstruct binding sites or motifs that are necessary for post-translational modifications. Despite these potential...Addgene blog Read our fluorescent proteins blog posts Download the fluorescent proteins 101 eBook Read...

  2. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...array of different techniques and protocols. In this post we will discuss the natural role of biotin, biotinylation...ligase (BPL) is responsible for this highly specific post-translational protein modification. Most organisms.... E. “The enzymatic biotinylation of proteins: A post-translational modification of exceptional specificity...

  3. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...again, this post has a lot of acronyms, so we've included a glossary at the end of this post! Figure...In our last post, we talked about the first base transversion editors: CGBEs, or C → G Base Editors. ...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of .... This is clearly the case when proteins undergo post-translational modification with fatty acyl chains...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources...

  5. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...right time. Like many of the posts in our Transferable Skills series, this post is not meant to be a comprehensive...details and vocabulary for this skill. What this post may do is help you realize whether or not you have... a conference poster session, you don’t necessarily want everyone who visits your poster to agree with...

  6. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...cloning we once relied exclusively on. In this blog post, I will go over some advantages, disadvantages, ...Independent Cloning (SLIC) in a previous Plasmids 101 post. Although SLIC may be more cost effective, Gibson...the Addgene Blog Browse All of Our Plasmids 101 Posts Read About Other Cloning Techniques Additional ...

  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... smear across your FSH vs reporter plot. In this post, I’ll walk you through how to interpret the antibody...  Note that for the flow plots discussed in this post, voltage has been adjusted appropriately and compensation...

  9. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about what I learned... got to summarize my experience in my first blog post! I highly recommend doing the same if you ever go...

  10. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...This post was contributed by Ignacio Amigo and María Hernández Sánchez from Crowdfight. In March 2020... resources on Addgene's blog Check out our blog post about remote collaborations Read our Transferable...science. Others are experienced researchers, such as postdocs and principal investigators. They also come from...IgnacioAmigoH     María Hernández-Sánchez is a postdoctoral researcher at Instituto de Investigación Biomédica...

  11. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    Published
    July 6, 2017, 1:59 p.m.
    .... To dive deeper into this field read our recent post “Cancer and the Immune System: Deciphering the Relationship...on the Addgene Blog Read Additional Cancer Blog Posts Addgene's Cancer Collection Pages Cancer and the...open-source! Impressions from the poster sessions During the poster session I got a chance to talk with...tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene...

  12. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...hundred trillion connections (synapses.)  In this post we’ll describe the AAV retrograde serotype (AAVrg...new tool, go forth and trace some neurons!  This post was originally written by Leila Haery in 2017 and...

  13. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...This post was contributed by Mary Tamer from BioBuilder, an innovative nonprofit bringing the study of...Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources on Addgene.org Guide to SynBio Assembly...chapter and an illustrated, downloadable classroom poster — with both of these resources road tested over...

  15. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...questions. Starting at the Broad Institute For his post-doc, Doench worked on using RNAi screens to learn...others at the Broad Institute here Read other blog posts about CRISPR Learn more about the Broad Institute...

  16. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...This post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes...F1000Research uses a novel form of peer review that occurs post-publication and is completely open; the reviewer...

  17. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...blog "Choosing a Good Mentor for Scientists". This post also includes a useful list of questions for a candidate...adding new scientists to their labs. Read the Other Posts in this Blog Series: Advice for Choosing a Research... who are dedicated and interested in science. “Postdoc hires are simpler because of their documented history...thrive during time spent as a graduate student or postdoc. Although the chosen research field is important...not be around much and your real boss might be a postdoc – this isn't bad, but you should know that this...

  18. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...are piloting this. Watch for my upcoming mentoring post in which I describe how to organize a successful...for help and advice. If you missed the first two posts in the series, get more advice on mentoring: What...

  19. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ...Aavery! Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new...
Showing: 261 - 280 of 694 results