We narrowed to 276 results for: sars

...upregulating HDR in mature tissues, as this process is necessary to precisely edit point mutations. In addition...

...correspond to the desired inputs. Such sensors do not necessarily have to be based on transcriptional regulation...

...sequence. This allows researchers to avoid making unnecessary base pair changes as would be the case with ssODNs...

... areas of active development, the first step necessarily dictates the direction and scope of the subsequent...

...: validate, validate, validate. This doesn’t necessarily mean that you yourself have to perform extensive...

...information could lead to waiting longer than necessary before starting your experiment, or if you start...

...established that DNA encoded all the information necessary for regulating and propagating life, there was...

...manner. Using both of these approaches may not be necessary for every CRISPR experiment. Off-target events...

... most important, targeting all genes doesn’t necessarily add value if you are addressing a well-defined...

...reporter is that not every transduced cell will necessarily manage to express CD45. Thus, if transduction...

...that best fits your needs and then modify it as necessary. And remember, if you end up creating a really...

...sequence for each unused promoter. This step is necessary to generate all of the overhangs needed for the...

...function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve cutting...

...PMC2877635. 39. Davis, R.L., H. Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts...

...27764162. PubMed Central PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field...

... human U6 promoter to drive expression, is it necessary to add a G nucleotide to the start of my target...