We narrowed to 274 results for: sars

1. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   Published

   May 31, 2016, 3:30 p.m.

   ...sequence. This allows researchers to avoid making unnecessary base pair changes as would be the case with ssODNs...

2. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   Published

   Oct. 27, 2020, 1:15 p.m.

   ... areas of active development, the first step necessarily dictates the direction and scope of the subsequent...

3. A History of Genome Engineering in Popular Culture

   Type

   Blog Post

   Published

   Feb. 25, 2020, 2:15 p.m.

   ...established that DNA encoded all the information necessary for regulating and propagating life, there was...

4. Antibodies 101: Validation

   Type

   Blog Post

   Published

   March 24, 2022, 1:15 p.m.

   ...: validate, validate, validate. This doesn’t necessarily mean that you yourself have to perform extensive...

5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

   Type

   Blog Post

   Published

   Nov. 2, 2021, 1:15 p.m.

   ...information could lead to waiting longer than necessary before starting your experiment, or if you start...

6. Sequencing Options for CRISPR Genotyping

   Type

   Blog Post

   Published

   Oct. 4, 2016, 2:30 p.m.

   ...manner. Using both of these approaches may not be necessary for every CRISPR experiment. Off-target events...

7. Custom CRISPR Screens & the Green Listed Software

   Type

   Blog Post

   Published

   July 11, 2017, 2:30 p.m.

   ... most important, targeting all genes doesn’t necessarily add value if you are addressing a well-defined...

8. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   Published

   May 28, 2024, 1:45 p.m.

   ...reporter is that not every transduced cell will necessarily manage to express CD45. Thus, if transduction...

9. Plasmids 101: Modular Cloning Applications and Kits

   Type

   Blog Post

   Published

   May 14, 2024, 1:15 p.m.

   ...that best fits your needs and then modify it as necessary. And remember, if you end up creating a really...

10. CRISPR 101: Multiplex Expression of gRNAs

    Type

    Blog Post

    Published

    Sept. 10, 2020, 11:45 a.m.

    ...sequence for each unused promoter. This step is necessary to generate all of the overhangs needed for the...

11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    Published

    Nov. 6, 2018, 1:12 p.m.

    ...PMC2877635. 39. Davis, R.L., H. Weintraub, and A.B. Lassar, Expression of a single transfected cDNA converts...

12. A Needle in a Base-Stack: Cas9 Structural Biology

    Type

    Blog Post

    Published

    June 4, 2024, 1:15 p.m.

    ...function. Stem loops 2 and 3 are not strictly necessary for in vitro cleavage, but they improve cutting...

13. Delivery Methods for Generating iPSCs

    Type

    Blog Post

    Published

    April 17, 2018, 1:37 p.m.

    ...27764162. PubMed Central PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field...

14. CRISPR/Cas9 FAQs Answered!

    Type

    Blog Post

    Published

    March 13, 2014, 4:08 p.m.

    ... human U6 promoter to drive expression, is it necessary to add a G nucleotide to the start of my target...