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TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....original, though its maturation is even faster. Published just a few days later, Mohan Balasubramanian and...-3188559/v1. Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance ...
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CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....expanded organoids. This work, and many others, pushed the field forward to where it is today. Below we...because of its potential for allogenic “off-the-shelf” treatment. In short, instead of using the patient... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....involves preparation of the tissue sample, several washes, and temperature changes to visualize the expression..., is available from Addgene. If you use these published protocols and want to share pictures, feedback... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog PostPublishedJuly 12, 2021, 1:15 p.m....Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....of growing collections of music. I still have a shelf full of CDs that have been gathering dust since ...tested for antigen binding. If starting from an established hybridoma line, the antibody can be converted... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...spacing between them so that they are easily distinguished. The UCLA Library offers some Powerpoint based... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m.... is fast and can cut in as little as 2 hours. Sheets, MB, et al., ACS Synth Biol. 2020. https://pubs.acs.org...Expanded serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....dCas9, also referred to as CRISPRa, was initially published in 2013 (Bikard et al., 2013, Perez-Pinera et ..., Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C,... -
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux
TypeBlog PostPublishedSept. 13, 2016, 2:30 p.m....landscape facing all scientists, both young and established, as they embark on or continue their careers .... and Social Media Content Specialist at Thermo Fisher Scientific Developing effective science advocates... -
The Materials Science of Optogenetics Experiments
TypeBlog PostPublishedSept. 17, 2015, 2:30 p.m.... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...it’s best to try those that have already been published first. Because behavioral optogenetics is performed... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....reels in the non-target strand of DNA much like a fisherman would reel in a big catch. It was originally suspected... Alyssa Shepard... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog PostPublishedJune 25, 2024, 1:15 p.m....Mathis et al., 2024) and concurrent research by the Shendure lab (Li et al., 2024) have highlighted how chromatin... Lalanne, J.-B., Nathans, J. F., Lee, D. S., & Shendure, J. (2024). Chromatin context-dependent regulation... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m.... that cannot be distinguished using conventional flow can (sometimes) be distinguished using spectral ... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring... -
Tips to Make the Most of a Scientific Conference
TypeBlog PostPublishedMay 23, 2019, 12:26 p.m.... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog PostPublishedOct. 23, 2018, 11:49 a.m.... genome that's then packaged into AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses... only the cultures with intact ITRs. I use a spreadsheet similar to this one to plan my AAV packaging ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog PostPublishedAug. 21, 2018, 12:31 p.m....well as premixed and ready-to-use reagents. Thermo Fisher Scientific Inc., Origene, and NEB® are all good...you. Happy cloning (and be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog PostPublishedAug. 4, 2016, 2:30 p.m....temperature for mammalian cells and one which diminishes the endonuclease activity of the enzyme. In vivo...epitope tagging). NgAgo survey Since it was first published in Nat Biotech in May 2016 (1), NgAgo has received... -
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments
TypeBlog PostPublishedSept. 23, 2014, 3:05 p.m....dissemination of your improved protocol. Many scientists, publishers, and small start-ups are turning to software ...– I didn’t get a smart phone until after I had finished my PhD and left the lab for non-bench work – but... -
Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....in the lab are more disheartening than seeing a potentially exciting result quashed by a bad standard curve...