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We narrowed to 790 results for: Cre

Showing: 141 - 150 of 790 results

  1. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...where DNA is being created, such as PCR amplification or bacterial growth. Created with BioRender.com....effort into our quality control (QC) processes to screen for and reduce these errors. These processes allow...  Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...process ensures that any potential sequence discrepancies can be caught prior to distribution. Catching...issue? At Addgene, a QC issue is defined as a discrepancy that we believe may affect the function of the...decisions when analyzing the wide variety of discrepancies encountered in plasmids deposited to Addgene...resolve. Sequencing and assembly issues Sequence discrepancies are not always a result of mutations in the ...

  2. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ... type of primer pair is also great for screening clones created with the same backbone but that contain...it's in the plasmid Can be used to screen clones that were created with the same backbone because primers...Molecular cloning requires some method of screening colonies for the presence of an insert. Traditionally...is, allowing you to use the same primer pair to screen for the presence of many different inserts. The...one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match insert-specific...test your colony PCR primers before using them to screen colonies. The best way to do this is by using your... background, the more colonies you will need to screen.   Saving clones for later culture: At this point...

  3. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...ve learned that increased accessibility is achieved through interconnected incremental changes. It was...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...identified and offered a number of solutions that increased accessibility, such as using couriers that refill...In some cases, the distributors broadly helped increase access in the country, and we can see a growth...re continuing to use our data and resources to increase accessibility to researchers around the globe....the materials in our repository. We started to increase accessibility to our repository by making it easier...Bacterial Transformation How to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid...

  4. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...article.) Modifying AraC to create BLADE The team modified AraC to create BLADE by replacing its dimerization...images created by the team (Fig. 2). These images are based on the concept of bacteriographs created about...bacteriograph of Michelangelo's "The Creation of Adam." This bacteriograph was created using 160 individual images...dimerize and activates transcription. The team created two constructs, among others: pBLADE-mCherry:...about a decade ago. To create these images, the scientists used a strain transformed with pBLADE expressing...instead of mCherry under the PBAD promoter. They created a bacterial lawn on an agar plate, put a mask over... that Di Ventura’s lab and Khammash's lab have created and shared with Addgene. You can find all the plasmids...

  5. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...Cancer cells find a way to grow haywire, access and create more vasculature to feed themselves, use the blood...the tumor microenvironment - a vital atmosphere created by the cancer cells to shield themselves from attack...cells of epithelial origin. Suppressing function, creating tolerance, and evading recognition are a few methods...cells into regulatory cells to suppress function. Creating Tolerance T-regs in the tumor microenvironment...HIF-1), which promotes the production of VEGF to increase angiogenesis around the tumor. This in turn regulates...signals released from the microenvironment help create tolerance beyond direct proximity to the tumor;...cells (DCs) and macrophages (M1 and M2) that help create welcoming environments for tumor cells spreading...

  6. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    Published
    Sept. 5, 2017, 1:58 p.m.
    ...mutations increased the accuracy of editing by up to 10-fold per allele. This is a 100-fold increase in editing...incorporate homo- or heterozygous mutations. 1. Increasing HDR accuracy with CRISPR/Cas-blocking mutations...find the 0.2-0.5% correctly edited alleles, when screening reasonable amounts of single cell clones. Furthermore...tedious business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000... together with the intended sequence change and screen a few hundred clones to find the right one Step... few hundred. From these two steps, you’ll be screening a total of a few hundred clones - Still better...efficiency of mutation incorporation falls with increasing distance from the cut site. If the distance is...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...that make major improvements such as those that increase plasmid yield and/or DNA quality. Table 1 below...sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed proteins ...methylate DNA lac Lac operon mutations Blue/white screening of clones   lacIq lac repressor overproduced...derivative. General cloning and storage, blue/white screening, leucine auxotroph. F- endA1 recA1 galE15 galK16...cloning and storage of common plasmids, blue/white screening. F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR...General cloning and plasmid maintenance, blue/white screening, partly restriction-deficient; good strain for...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...encoding Gag and Pol, increasing the number of recombination events necessary to create a replication-competent...Enable Genome-Scale, Knockout Screening Read about Genome-Wide Screening Using CRISPR/Cas9 Additional...shows how the lentiviral genome was condensed to create the 2nd-generation lentiviral system (Figure 2)...safer than the original system, the possibility of creating a replication-competent virus via recombination...possibility and enhance biosafety, Dull et al. created the 3rd generation system (Figure 3), which differs...Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the lab - the Trono lab’s lentiviral...both for targeted modification and genome-wide screening.   Lentiviral vectors in the clinic Since lentiviral...

  9. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ...CBEs). But unlike CBEs, CGBEs then excise the U to create an abasic (empty) DNA site using either fused or...nucleobase component of the nucleoside inosine. Created with BioRender.com. Inosine excision leads ... able to edit A → Y (Y = T or C) in 67.17% of screening cells (Tong, Wang, et al., 2023). Based on this... for A → X base editor (X = any nucleotide). To create a more specific A → C base editor (ACBE), they ...Black arrows indicate natural cellular processes. Created with BioRender.com. The first deaminase-free...either to directly accomplish the base edit or to create the substrate for a base excision repair pathway...nCas9. First, MPG removes the G base from DNA to create an abasic site. Like in most other base editor ...

  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...Marker B. Created with biorender.com.   The goal of flow cytometry is to use increasingly more stringent...populations that are B+ and one population that is B-. Created with biorender.com.   If it’s hard to conceptualize...imagining (or drawing) a quadrant over the dot plot. Created with biorender.com.   Of course, this is quite...al., 2017 to identify mDCs and pDCs. Used under Creative Commons license.    In the third plot, anti-CD14...visualize the different populations. Used under Creative Commons license.    Pro tip! Subtypes of immune...the percentage of cells in each gate. Used under Creative Commons license.    Contour plots  FACS data...data from Jhunjhunwala et al., 2015. Used under Creative Commons license.  Histograms  For analysis...
Showing: 141 - 150 of 790 results