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We narrowed to 559 results for: ESP

Showing: 141 - 150 of 559 results
  1. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...the liver (A). In general, RNA levels tend to correspond to protein levels, so you perform immunohistochemistry...to be present and recognizable by your antibody despite the manipulation. Additionally, not all sample ...
  2. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...position, what will you need? Multiple first or corresponding author publications (published, not in preparation...audiences and if you pay attention to how the audience responds to your presentations, you will become a more ...experience and learn about modern pedagogy techniques, especially active learning. Serve as a daily mentor for ...
  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Create a monoclonal Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal..., so some of the cells will still appear green despite having active Cas9 (see tip 2). Pro-Tips 1. To ...about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research opportunities...
  4. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...infections. Anything that generates an antibody response in the immune system is referred to as an antigen... is expressed at different times of the immune response and initiates different immune cascades. In the...antibody to recognize. This triggers an immune response within the animal, and its B cells will churn ...
  5. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...- a major 397 nm peak and minor 475 nm peak, respectively. When wtGFP is illuminated with UV-violet light...I197H) (3) were derived from DsRed and mRFP, respectively. When illuminated with violet light, both variants...field of super resolution microscopy. PA-FPs, especially the monomeric, reversible type, are very well...
  6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...dyes interact with specific receptors is key, especially for G-protein-coupled receptors (GPCRs). Mutations...also vary in their brightness, depending on the respective fluorochrome. Brightness is impacted by both ...between fluorescent and non-fluorescent states in response to a specific stimuli, which leaves only a small...
  7. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...systems, naming the new systems PE4 and PE5, respectively. These systems improved editing efficiency by... RT and the S. pombe Tf1 retrotransposon RT, respectively. Their small size comes at the cost of improved...further evolved the Tf1 and M-MLV RT enzymes, respectively, to obtain PEs small enough to deliver with ...
  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...bacterial phytochrome BphS which produces c-di-GMP in response to far-red light (730 nm). This nucleotide triggers...high-affinity interacting proteins Coh2 and DocS, respectively. The use of far-red light is of particular interest...Zhou et al., 2018). pdDronpa dimers dissociate in response to cyan light (500nm). By fusing the two pdDronpa...
  9. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    Published
    Jan. 11, 2024, 2:15 p.m.
    ...has been considering a gradual move off X as a response to major platform changes, such as limiting access... and we acknowledge there may be a variety of responses to this decision. However, our decision allows...
Showing: 141 - 150 of 559 results