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We narrowed to 729 results for: NSI

Showing: 141 - 150 of 729 results

  1. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...individual neurons, including dendritic and axonal extensions and, most importantly, any connections the neurons...neuroanatomy and neural connectivity, providing valuable insights for neuronal communication. Viral vectors and...synaptic step, thus preventing further widespread expansion of RABV. This improvement resulted in a clearer... Not all measures of transport efficiency are consistent which may be caused by the diversity of techniques...the field of AAV vectors. Conclusion An ideal comprehensive toolset for neuronal tracing and network identification... wheat germ agglutinin (WGA) as a new, highly sensitive retrograde tracer in the rat brain hippocampal...nucleus Rabies and neuronal tracing Important considerations when using AAVs Resources on Addgene.org ...

  2. Finding Your Science Policy Path

    Type
    Blog Post
    Published
    Sept. 22, 2020, 1:15 p.m.
    ...science policy community is the one that was consistently welcoming. This bears repeating. Individuals... early career researchers who have decided to transition out of academia from graduate school or a postdoc... came to DC to do either a sabbatical year or transition away from academia as tenured professors. There...Day One Project for some example proposals and consider applying for their accelerator for some hands-... to future positions, but will also make your transition from academia to the policy world much smoother...smoother.  Action Items:  Explore this comprehensive resource developed by Dr. Kelly Singel which provides...

  3. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...someones) taking on these responsibilities on a team to which you belong, consider stepping up. Otherwise... function. When a select group of people are responsible for a group of specific processes, they must ...tasks done. At Addgene, the Scientist Team is responsible for guiding new deposits into the repository,...roles they’re meant to fill and what their responsibilities are. That’s a subtle but important distinction...- and really can’t - be done alone. So, if you consider yourself part of the scientific community, get...

  4. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... This post will go over some of the general considerations you should review before using an antibody....necessarily mean that you yourself have to perform extensive validation experiments for every antibody that...data can be acceptable. However, it is your responsibility to determine what counts as adequate validation...methods. These approaches have relatively low sensitivity, which can be problematic if you are trying to... your antibody’s trustworthiness. You may also consider sharing your validation attempts by other means... test an antibody (perhaps one from Addgene!), consider sharing your results so that others in the community...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    Published
    Nov. 22, 2016, 3:30 p.m.
    ...multiple high-profile publications. My classmates consisted of graduate students, post-grads, and early career...topic by the moderators. The assignments, which consisted of reading that class’s section of a manuscript...given the opportunity to sign up for a writing intensive weekend in Washington D.C. at the ASM headquarters...Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a manuscript...and they should also be easily understood and consistent in design and layout. 2. Be concise in your writing...

  6. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    Published
    Nov. 1, 2017, 2:04 p.m.
    ...aspects of the subject you're writing and then condensing that and making it accessible to students.” Making...for example, cutting open a heart to look at the inside. We do a lot of interactives and videos, as well...specimens.” And since the eScience warehouse is onsite, Caitlin can visit the operations team to make ...curriculum, but has taken on other new and exciting responsibilities.“I’d say maybe 25% of my day is still what...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

  7. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ...will discuss some of the features you may want to consider as you make your choice. The first and most important...selection marker or antibiotic resistance. Next, consider the expression level of your construct: would ...pcDNA3-EGFP). If you can get away with generating transiently transfected cells, you will not need a mammalian... lentiviral or a retroviral backbone. This is considered to be a more robust expression system and has...are several types of yeast expression vectors. Consider your applications and experimental design. You...

  8. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ...society 6)   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some...magazines or newspapers or other places online, and consider how those stories are being told. Twitter can ...science blogs written by practicing scientists. 3)   Consider starting up your own twitter feed or talking about... both your plasmids and your research stories. Consider practicing communicating your research by guest...

  9. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...know all about antibiotic resistance genes, let’s consider another basic element of any plasmid: the origin... intend to use, and whether or not you need to consider your plasmid's compatibility with one or more ...copy number varies. See below for additional considerations. *Requires pir gene for replication (reference... external factors contribute as well. These considerations are especially useful to keep in mind if you...affect copy number. Some ori's are temperature sensitive; others ori's can be "tricked" into amplifying...

  10. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...University of North Carolina (plasmids at Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds...attract other proteins involved in their growth and extension. Now, they split EB1 into two halves (Fig. 1):...RNA localization in mammalian cells. These tools consist of cobalamin-binding riboswitches that bind to ...single base-editors has resulted in the rapid expansion of the genome editing toolkit. However, a challenge...quantitative editing assay. To help accelerate the expansion of genome editing tools, the Reuben Harris lab...
Showing: 141 - 150 of 729 results