Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 885 results for: ANT;

Showing: 421 - 450 of 885 results

  1. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...expression. Experimental output is measured and quantified. Created with BioRender.com.   Optogenetic...2020). Light-gated ion channels are useful when you want to activate or inhibit specific neurons without ...pathway. Photoswitches are great for teasing out important branch points along a signaling cascade that may... activated. Created with BioRender.com.   Important considerations for using optogenetic tools Once...optogenetics or biosensors in one system. There can be substantial overlap in the activation curves (Marshel et ... fidelity with the stimulus. For example, if you want to stimulate at a fast frequency without missing...can wreak havoc on your experiments, so it is important to understand the details of how each tool performs...

  2. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...findings can be predictably applied to future work. Importantly, no one should expect that every study should...who have complaints about the unreliability of antibodies. IPI is working to address this issue directly...directly by creating well characterised antibodies against all human extracellular proteins with community-...research tools. BenchSci collates information about antibodies while FPbase curates information about fluorescent...and use computer based analysis techniques, an important component of reproducibility is making sure that...figures. Cause #2: Hidden “negative” data We all want to be able to tell a good story when presenting ...journal’s perspective, probably not what most readers want to read. As Susanna Bachle points out in her talk...

  3. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...reporter-based) each come with their advantages and disadvantages. Sanger sequencing is simple and the...1B below). Alternatively, if an attB-attP pair is anti-aligned (sites facing opposite directions) on a ...from multiple primer pairs, one can infer the predominant state of registers within a sample using a metric...multiple registers. However, it is only semi-quantitative. It won’t tell you the distribution of states...states in a sample; instead, it will suggest the predominant state of registers in a sample based on a similarity...of inputs (see Figure S9 of our paper (1)). We anticipate that, depending on the context and application...

  4. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ... have pushed the boundaries in neuroscience by granting targeted control over neuronal activity. While..., both techniques offer researchers different advantages and limitations.  The four main factors in which...studies, however, require a permanent intracranial implant to be surgically installed to allow for light delivery... delivery. Chemogenetics poses the advantage of being less invasive and more flexible as it relies on ...Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation ExogenousEasily...controlled EndogenousNot easily controlled Unique advantages Mapping the brainDissecting circuits Studying...

  5. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...direct, specific and sensitive detection of the plant hormones strigolactones By Fabienne Furt Current... detect strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers...fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors also showed that... known to trigger seed germination of parasitic plants, this approach was also used to build and validate...Qi's lab, allows simultaneous gene activation in plants.  FnCas12a, unlike other Cas12a nucleases identified...in moderate thermophiles.  These evolved SpCas9 variants have improved activity on NAG PAMS and reduced...

  6. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...ve realized my figures are just as important, if not more important, than my writing. Initially I didn...design software, like Adobe Illustrator, and I didn’t want to pay for these programs either. But with a little...presentations, manuscripts, or social media. If you want to draw your own figures Google Drawings Google ...through your university or your public library. If you want to use pre-drawn images in your figures Biorender...New icons can be requested, although there’s no guarantee Biorender will create it. Additionally, you can...

  7. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...ever been the target of an ‘-omics’ study or is resistant to specific compounds, links to publication references...cross-references with cell line catalogs and collections. Importantly, Cellosaurus also identifies problematic cell... the most common contaminator line.  For those wanting to access Cellosaurus programatically, their API... helpful information on the known features and relevant literature, or to decide which line is best for...scientists all over globe, with some surprising, and important, findings being shared. For instance, recently...around! If you’re excited about this resource and want to learn more, check out the Cellosaurus FAQ! References...

  8. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ... our ReFigures on the therapeutic use of anti-CD47 antibodies in immuno-oncology. Funding agencies such... Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications as in this ReFigure... ReFigure to coherently save analyses with the relevant figures from a literature review. No more hunting... and good for science ReFigures are published instantly and are editable by the author at any time. Insights...preprints, data, software, micropublications) in grant proposals. ReFigures are one such research product...

  9. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... both of these cases, transposons combine the advantages of viruses and naked DNA while eliminating some...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...transposase (as determined by transposition assays of antibiotic-resistance genes conducted in HeLa cells). In...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...as a nonviral vector for CD19-directed chimeric antigen receptor (CAR) therapy. SB is a valuable tool and...

  10. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    Published
    Sept. 18, 2018, 1:33 p.m.
    ...reporter can also quantify Cas9 cleavage, thus exhibiting its versatile utility in quantifying multiple gene...versatile in vivo fluorescent reporter for rapid quantification of base-editing and Cas9 cleavage activity ...several cell types is the lack of a rapid and quantitative editing assay. To help accelerate the expansion...plasmid that allows for rapid fluorescence-based quantification of base editors that utilize dCas9 fused to...frame-shift mutation within the mCherry reporter. Quantification of editing efficiencies simply involves tracking...

  11. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...restriction sites are present in a given sequence. You want to choose enzymes that: Do not cut within your...Next, we need to examine the DNA sequence that we want to amplify and design primers that will bind to ...primer design: Because we are cloning an ORF, we want to clone from the start codon (ATG) to the stop ...reaction Run PCR to amplify your insert DNA. It is important to use a high fidelity polymerase to minimize .... The fidelity of the polymerase becomes more important the longer the expected PCR product is. You should...used. No matter which polymerase you use, it is important that you sequence the final product. Additional...

  12. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ...treated a tetracycline resistant plasmid, pSC101, and a newly developed kanamycin resistant plasmid, pSC102,...with EcoRI and selected for E. coli transformants that were resistant to both. When this proved successful...generic term for any extrachromsomal hereditary determinant”. His proposal, however, was basically ignored...was the tool of choice for molecular biologists wanting to study bacterial genetics. This all changed thanks...

  13. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...physiologically-relevant systems, to catch all the important interactions. It’s also important to determine...speak, with collaborators in Paris and New York. We want YOU to join the fight against coronavirus We are... a lot of background in our study - so you might want to be careful with these clones (and help us figure...been quite the administrative headache receiving constant email requests and tracking their fulfillment ...

  14. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    Published
    Sept. 12, 2019, 1 p.m.
    ...-AAV-syn-jGCaMP7c variant 1513-WPRE (105321-AAV1) pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE (105322-...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific...relative to baseline fluorescence, as the GCaMP7f variant, and is approximately 5-fold brighter than GCaMP6s...GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest resting fluorescence...fluorescence from inactive neurons is reduced. This variant has an approximately 12-fold increase in brightness...

  15. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    Published
    Sept. 3, 2019, 1:15 p.m.
    ... mAID and expressing the essential plant protein TIR1 in non-plant cells, which interacts with the cells...Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins to conditionally and reversibly...a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ...engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used genome editing tool...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were...

  16. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...people use is the built-in whiteboard, in which participants can contribute and sketch on the board without...control panel on the top of the screen and every participant get to annotate things as they come up in their...time breaks up the monologue a little and gives participants something else to focus on. Use collaborative... creativity are pivotal in writing publications/grants too. As described by several works (Wuchty et al...fades away the longer we are on it – so it is important to take breaks. After 1 hour (or another designated...be done randomly or manually assigning which participants to group together. Breakout Rooms can be used...

  17. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    Published
    July 17, 2018, 12:23 p.m.
    ...other professors at the University of Barcelona wanted to develop strategies to make our classes more ...thinking about how to solve this problem. I didn’t want to face another group of sleepy students at 8 a....engaging card game could help me get the engagement I wanted. I therefore started planning a game that would...following lessons. And let me tell you more, they had brilliant ideas on how to solve many therapeutic and pre-clinical...educational nature of this material are welcome and if you want to receive our modifiable powerpoint file, please...University of Barcelona. He is also founder and consultant at FRESCI particularly interested in advanced...

  18. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...us take comfort in the fact that it’s often not quantity, but quality that really matters. Well, it turns...another example, neuronal gene expression by an anterograde AAV was shown to drop off drastically with lower...concentrating your AAV above your desired titer and want to dilute it, err on the side of caution when adding...References 1.Clark, K. Reed. "Recent advances in recombinant adeno-associated virus vector production." Kidney...PMID: 11841606. 2. Schultz BR, Chamberlain JS. Recombinant Adeno-associated Virus Transduction and Integration...PMC2574934. 3. Zingg, Brian, et al. "AAV-mediated anterograde transsynaptic tagging: mapping corticocollicular...

  19. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...time, he is pursuing a plant engineering project at BosLab. Once he optimizes plant cell culture and transgenesis...transgenesis he wants to share his knowledge with the community by teaching a DIY plant engineering workshop...and to see old pictures of the lab!). By 2016, we wanted to be taken seriously so we made it official by...ecology but she became a member at BosLab because she wants to engineer microbes. We’ve ordered a few yeast ...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...Imaging by Coat protein Knock-off) instead takes advantage of the ribosome’s movement. When the ribosome ... reporter. Delayed translation is known to be important in development, and Halstead et al. used TRICK...mRNA and keeps it in a single field of view for constant tracking. You can then directly monitor multiple...that can recruit GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the...second. They also explored the effects of 5’ UTR variants in translation of cell cycle protein Emi1, showing...and 3’ UTR. Before beginning experiments, it’s important to verify that these changes do not impact translation...

  21. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...This post was contributed by guest bloggers Samantha Zyontz and Neil Thompson from the MIT Sloan School...pulled the journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology...after controlling for journal. And so, while our quantitative analysis cannot rule out all other explanations...along the way. Many thanks to our guest bloggers, Samantha Zyontz and Neil Thompson. Special thanks to Addgenies...for helping compile the data used in this work. Samantha Zyontz is a Ph.D. candidate at the MIT Sloan School...ssrn.com/author=1232466.     Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT...

  22. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...scientists to both quantify Cas9 activity and sgRNA efficiency as well as enrich for mutants through the presence... created CRISPR fluorescent reporter assays to quantify targeted Cas9 cleavage, and repair through homology... in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses the Piggybac...presence of a fluorescent reporter. Lastly, once a mutant is identified, the PB transposase can easily and...effective gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References...

  23. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...diverse libraries of genetic variants. Several methods for generating mutant libraries exist, but none provide...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components...Assisted Continuous Evolution (PACE) method requires constant turbidity of the culture, so bacteria must be ...novel genotypes that result in a spectinomycin resistant phenotype in bacteria. While there are many known... feature which they used to generate bacteria resistant to both spectinomycin and streptomycin, but could...

  24. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    Published
    Jan. 18, 2018, 2:34 p.m.
    ...expression and largely defines the amount of recombinant protein that will be produced. Many common promoters...and steroid regulated promoters commonly used in plant research. Promoter Subtype Promoter Example Activator...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...various eukaryotic cell types (mammalian, yeast, plants, etc.) require unique promoters and there is very...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....low leakiness. Inducibility level How much do you want to induce transcription of your target gene? The...

  25. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...Plasmids 101 posts here. Now that we have covered antibiotic selection here at Plasmids 101, we can talk about...amino acids (called the α-peptide) to a lacZΔM15-mutant bacterial cell in trans complements the mutation...they should be. Don't rush the process: It is important to give your plates enough time for any intact...identify colonies that not only have your plasmid (antibiotic resistance), but also confirm those plasmids ...α-peptide DNA disruption in the blue-white screen. Antibiotic selection is also used in conjunction with this...select for plasmid-containing cells without using antibiotic resistance. These rely on cell lines susceptible...

  26. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...considerations. First, it is important to establish the type of audience you want to reach with your paper... your paper to your preferred  journal, it is important to check the journal’s guidelines to authors and...catalogue numbers and manufacturers’ details for antibodies, plasmids (like your Addgene plasmid #), and ...an identifier (a bit like a GenBank ID) to all antibodies, animal models, and software tools in the Materials...journals and their scientific communities aim to significantly improve the reproducibility and reliability ...

  27. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ... ask” once you have found someone you admire and want to learn from. Check out Joanne's Reddit AMA Who... colleagues that have the training or skills you want to learn. Mentors can be senior to you or peers ...mentors from your current organization but also important to build relationships outside your organization...confidential topics Broaden your network Take advantage of formal programs A formal mentoring program ...mentoring circle. This type of program has many advantages and I am seeing a lot of success at universities...feedback. The best thing to do to make your mentor want to keep working with you is report back with progress...

  28. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is our fourth... and induce their effector functions. It’s an important process that we need to understand so that we ...source of IL-36 cytokines in the skin making it important to study what is happening within the keratinocytes...genes from the X chromosome are expressed at significantly reduced levels in women with TS (Trolle et al...nucleus,” says Dr. Terry Hébert, a professor and assistant dean of biomedical science education at McGill.... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...

  29. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ...on the conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...stromal cell modifications, stromal cells are also constantly communicating with a tumor, proving once again...malignancy but also incite different organs to become malignant. Exosomes also contain miRNA (micro RNA) and other... case, exosomes may transport miRNA from drug-resistant cells to sensitive ones. Though this process is..., the microenvironment backstage play just as important of a role if not more.  Many thanks to our guest...

  30. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    Published
    Aug. 5, 2021, 12:15 p.m.
    ...the brain. “We hope that iGluSnFR3 will enable significant steps forward in our understanding of synaptic...Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect...hydropersulfides modulate protein activity and have important roles during oxidative stress. To monitor this...properties. Her method takes a pooled library of mutant sensors expressed in a mammalian cell line engineered...engineered to be electrically excitable. The mutants are co-expressed with a photoconvertible fluorescent...photoconverted, sorted, and sequenced to identify the mutants for further enrichment. Tian notes that this platform...
Showing: 421 - 450 of 885 results