Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 750 results for: Tro;

Showing: 421 - 450 of 750 results

  1. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals...Massachusetts Nonprofit Network Excellence Award for our electronic MTA system. We also started our blog and YouTube...biology and cloning, since they have reasonable introductions to lots of molecular biology,” — Reddit commentor...

  2. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...found that the study saved lives. Reduced use of estrogen plus progestin hormone therapy resulted in 76,...they worry about needing more study subjects and control groups. The important point, however, is that cost...Evoloving Check Out Extracurricular Activities for a Strong Science Career Path Additional Resources Check...

  3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    Published
    June 15, 2017, 2:30 p.m.
    ...pitfalls of these experiments are discussed here. In vitro tests for aggregation The tendency of purified fluorescent...concentration 50% of the protein forms dimers. Finally, spectroscopic methods (based on fluorescence polarization)...respectively, are used as positive and negative controls for proper ER structure. Based on these data, ...

  4. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...attached to a substrate. These in vitro methods allow for a well-controlled environment, but they do not mimic...large number of emitted photons before they are destroyed. Factors that affect photostability The photobleaching...

  5. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ... molecule degraders” recruit the proteasome to destroy targeted proteins. We previously developed small...lines Treat parental cells (or cells expressing a control -- for example, an FKBP12F36V-tagged LACZ) with...: dTAG-7 and dTAG-13 Erb et al. Transcription control by the ENL YEATS domain in acute leukemia. Nature...

  6. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    .... Unfortunately, LinkedIn has spam profiles and trolls. People don’t like to connect with complete strangers... much better to use your network to request introductions to people of interest for such discussions.  ...connection can help you gather information, make introductions, and give advice for your interviews.  Pro tip...

  7. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...communicating the results of the work. Yes, those control experiments are important — but without letting...and finish that experiment (and don’t forget the controls!). And when you’re ready to write up the paper...blog Summer SciComm Series: Modes of Communication Intro to Our Summer SciComm Series!...

  8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...,000 people globally. Pustules form from the uncontrolled activation of the interleukin-36 (IL-36) cytokine...macrophages, T cells, and the recruitment of neutrophils. Though there are IL-36 receptor inhibitors in... significantly reduced levels in women with TS (Trolle et al., 2016). Using lentiviral vectors from Addgene...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...effective as it can result in the retention of introns or the removal of exons. Disrupting promoter or.... Find CRISPR plasmids at Addgene! Knock-in: Introducing tags or stop signals Once Cas9 generates double-strand...breaks, a new DNA sequence can be knocked-in, or introduced, into the break sites. Knock-in experiments are...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor (your supervisor... typically includes a couple of sentences of introduction to my area, a line about the research I will...you can find some great information on ELISA troubleshooting and multiplex assays.   Additional Resources...

  12. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...sequence (Green et al, 2014). The second was an in vitro paper-based platform that allowed gene circuits ... an extensive infrastructure of molecular and electronic companion technologies that has transformed the...CRISPR/Cas9-mediated genotyping, as well as our electronic reader that provides portable quantification ...

  13. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...expression, bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression...recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, choose the NEB Stable strain...

  14. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...find a title, author names and affiliations, introduction, methods, results, conclusions, and acknowledgements...flanked by two equally-sized columns, where the introduction, methods, conclusions, and acknowledgements ...the work. The other poster sections like the introduction, methods, and figures flank the central region...

  15. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   Figure 2: Schematic...single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways...

  16. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    Published
    April 28, 2016, 2:30 p.m.
    ... recombinase.   Once the SSR of choice is introduced, recombination can proceed either by first utilizing...alone rescues the phenotype being studied. Introduction of a conditional point mutation or functional...transduced reporter gene. Generating a FLEx switch to control expression of an optogenetic reporter would ensure...

  17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...replacements, small insertions, and deletions) without introducing DNA double-strand breaks (Anzalone et al., 2019... a multitude of configurations can be used to introduce specific target modifications. Since this means...University of Zurich (Switzerland), where he developed a strong passion for computational biology. His expertise...

  18. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...pitch,” the 30 - 60 second blurb you will use to introduce yourself and what you do. You might want to include...research. Try to avoid jargon in this initial introduction. During the conference When you meet someone...is what their project is. Or maybe you want to introduce yourself to a scientist whose work you are familiar...

  19. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...the resistance cassette has been placed into an intron.    Figure 4: A targeting vector to create... Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed. You'd...

  20. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...background without an introduction. If someone approaches your poster, introduce yourself and ask about...have extra time to practice at the actual venue. Introduce yourself: I always find it odd when I walk up ...

  21. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...selecting an advisor, it is always a good idea to introduce yourself to a few members of your desired department... potential PI should go something like this: Introduce yourself, brief educational background, and that... meetings can still be a great opportunity to introduce yourself and learn more about your schools of ...

  22. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...evaluated what lessons I had learned – call it “troubleshooting.” Trying out new experiments and new ideas ...erlenmeyer flask with gold glitter spray paint to make a trophy for the winner. We promoted the contest for about...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

  23. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...complicated cloning of homology arms. MMEJ: An Introduction There are three primary methods for repairing...NHEJ is error-prone, such a system is likely to introduce additional nucleotides flanking the insertion....the DNA ends anneal incorrectly, MMEJ may also introduce, substitute, or delete nucleotides in addition...

  24. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    Published
    Sept. 23, 2014, 3:05 p.m.
    ...comment, annotate, and edit protocols. After my introduction to protocols.io, I decided to research what ...orders, tracking students and research projects, electronic lab notebooks and protocol management. Though...More Reading: How to manage scientific data: Electronic lab notebooks compared with Google Docs and wikis...

  25. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of the ...are great indirect immunodetection tools is their strong signal strength. This signal strength is due in...ladders from Penn State University Use Nanobodies to Control Protein Activity Learn more about protein tags ...

  26. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...average of 4 sgRNAs per gene and 1,000 non-coding control sgRNAs. This library was designed to improve on-target...CRISPRko libraries are highly effective at identifying strong hits in genetic screens, they do have several limitations... Luke A., et al. "Genome-scale CRISPR-mediated control of gene repression and activation." Cell 159.3 ...

  27. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...preferred platform before you start can save a lot of trouble in the long run.  Selecting the method Once you... Requires regular content production. Internet "trolls"   More resources on the Addgene blog:  Summer...  Summer SciComm Series: Cognitive LoadIntro to our Summer SciComm Series    ...

  28. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...impossible for a career scientist such as Zimmer to introduce any one of these topics without some editorializing...fellow scientists--but that doesn’t make his introduction to these topics any less effective. Who is this...non-scientists in your life, the book may be the introduction they need to grasp the challenges you face in...

  29. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ... and negative (gray) droplets. The no template control wells should have very few positive droplets. In...positive copy per microliter in the no template control wells. You’ll also notice a reduction in the number...assay, so cross contamination into a no template control (NTC) well is common. Here are some steps you can...

  30. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively large. Cloning...separate plasmids allows researchers to partially control expression levels of each gene by altering the ...genes (either viral genes or minigenome) are under control of a T7 RNA polymerase promoter sequence. Thus,...
Showing: 421 - 450 of 750 results