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We narrowed to 920 results for: Gene

Showing: 561 - 600 of 920 results

  1. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...). Efficient site-specific integration of large genes in mammalian cells via continuously evolved recombinases...Over 75,000 pathogenic genetic variants have been identified in humans and cataloged in the ClinVar database...editing tools. We’ll summarize the major ones in Addgene’s repository later in the post. Advantages of prime...by double strand breaks has been widely used to generate precise changes. However, the efficiency of Cas9...Prime editing has a lot of promise for treating genetic diseases. In our summer Hot Plasmids post, we covered...demonstration of prime editing used to rescue a genetic disease phenotype in an animal model, which was...s41587-022-01527-4 Additional resources on the Addgene blog PRIDICT: Predicting Efficiencies of Prime...

  2. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...Resources on Addgene.org Addgene’s Viral Services Addgene’s Virus Protocols Addgene’s Chemogenetics Guide ...system.   Figure 2: General overview of chemigenetic tools. A genetically encoded component is delivered...Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics Fluorescent... within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors. These tools...on this later). Chemigenetic sensors take a hybrid approach and utilize both a genetic component as well... system. Single Fluor Chemigenetic Sensors This system consists of a genetically expressed enzyme, typically...about how viruses can be used for chemigenetics, biosensors, and generally as biological tools, our blog ...

  3. New Viral Vectors - Winter 2025

    Type
    Blog Post
    Published
    Jan. 28, 2025, noon
    ...AAV1 Chemogenetics Scott Sternson New viral prep pAAV-CaMKIIa-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan...mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-hSyn-hM3D(Gq)-mCherry AAV1 Chemogenetics Bryan Roth... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...but browsing the 49 new preps available in the Addgene viral vector repository with a nice cup of hot ...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor... serotype pAAV-hSyn-hM4D(Gi)-mCherry AAV1 Chemogenetics Bryan Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato...prep pAAV_hSyn1-SIO-stGtACR2-FusionRed AAV5 Optogenetics Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...learn what Addgene can do to help. More resources on the Addgene blog Twenty Years of Addgene Sharing: ...researchers around the globe. Addgene’s educational resources As Addgene built out our scientific support...other videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel...about virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing...When Addgene opened in 2004, we focused on creating a repository that made it easy to share plasmids,...accessible, due to a wide range of logistical barriers. Addgene worked to identify, reduce, and remove these logistical...accessible weren’t always the same thing, shaped both Addgene’s founding and our growth into the company we are...

  5. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's Advisory...lab. In June 2014, Dr. Fujii joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively ... enChIP through Addgene. Retroviral Expression System for enChIP using CRISPR Addgene: Your lab recently...the plasmids of these systems to Addgene in the near future. Addgene: Has your lab been working on any...first knew Addgene around 2010 and found the scheme very nice at first sight in that Addgene takes care...ourselves without Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted...Hodaka Fujii lab's Addgene page and click the "Subscribe to Alerts" link in the Addgene Alerts box. References...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...expected to grow with the ever-expanding genetic toolbox generated by the continuous effort of numerous scientists...). GFP is the most popular, most widely used genetically encoded fluorescent probe. Several factors contribute...probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are, however, ...1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant, mEGFP (Zacharias..., followed by an intramolecular reaction that generates the chromophore (Tsien, 1998). Importantly, this...Thorn, 2017). These fluorescent analogues are generally added to the bathing solution and require uptake...adding GFP may alter diffusion rates. Smaller genetically encoded probes are based on cofactor binding ...

  7. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful...deposited many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA...gRNA libraries. Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types...types exist.  When you buy a pooled library from Addgene, you can either order a DNA pooled library, which...single tube, or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but ... A) Amplification, validation, and lentiviral generation of a pooled library. An aliquot of the pooled...experiments. Pooled library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw ...

  8. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ... institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal agreement...materials from Addgene can qualify. We also reward improvement against one’s own record Mention Addgene Master...question? Refer them to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the...between a TTO and Addgene, whose terms apply to all future deposits of materials with Addgene Master Reception...between a TTO and Addgene, whose terms apply to all future materials requests from Addgene MTA: material ...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer...arrival of the newest plasmid you requested from Addgene...the plasmid that will help you run that last ...

  9. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...Initiatives for the Personal Genetics Education Project (pgEd). Advances in genetic technologies and other ...broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical School...existing inequalities and injustices. The Personal Genetics Education Project (pgEd), where I currently work...work, strives to broaden the conversations about genetics and facilitate dialogue between scientists and...the personal, social and ethical implications of genetics. In these conversations, pgEd strives to be inclusive...and empowered to make their own decisions about genetics, both as individual consumers of technologies ...

  10. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...As a tech at Addgene, part of my job, in addition to preparing DNA and generating/replenishing kits, ...information from the workshop. Future workshops for Addgene Addgene strives to be a great resource for the research...Technician at Addgene who is interested in medicine and music.       Additional Resources on the Addgene Blog ... use what we learn to improve processes in the Addgene lab. In this post, I’ll tell you a little bit about...excited to go there because both LabCentral and Addgene aim to make scientific advancement easier. NEBNext...extract libraries from previously fragmented DNA.The general process for library preparation using this kit ...

  11. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...your desired edit are all you need to generate a knock-in.   General considerations for designing a repair.... A Mouse Geneticist's practical guide to CRISPR applications. Singh P, et al. Genetics 114.169771; ...accurate mechanism for DSB repair. The process is generally error-free provided the DNA template used for ... DNA to the target sequence to be modified. In general, the insertion sites of the modification should...bases can be difficult, thus dsDNA plasmids are generally preferred for large insertions such as fluorescent...efficiency with circular plasmid templates is generally low; to increase the frequency of edits, researchers...targeting region from the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA templates...

  12. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...Libraries Resources on Addgene.org Addgene's Guide to Plasmid Pooled Libraries  Addgene's Pooled Library Collection...chemical transformations are generally cheaper and easier. One library that Addgene distributes with a chemical...contact us at deposit@addgene.org. Happy amplifying! More resources on the Addgene blog Genome-Wide Screening...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...recombination and loss of plasmid diversity, so Addgene strives to distribute aliquots of the original ...requesting scientist receives a pooled library from Addgene, they’ll need to amplify the sample for further...developed for each individual pooled library; generalized amplification protocols tend not to work as well...

  13. The Twelve Days of CRISPR

    Type
    Blog Post
    Published
    Dec. 22, 2020, 2:15 p.m.
    ... CRISPR, Addgene gave to me: a primer on prime editing.  On the second day of CRISPR, Addgene gave to ...On the fourth day of CRISPR, Addgene gave to me: ways to use optogenetics to control CRISPR editing.  ... day of CRISPR, Addgene gave to me: updates on base editing from the first generation of base editors ...the eighth generation (and dual base editors, too!).  On the seventh day of CRISPR, Addgene gave to me... of CRISPR, Addgene gave to me: anti-CRISPR proteins!  On the ninth day of CRISPR, Addgene gave to me:...🎶 On the 12 days of CRISPR, Addgene gave to me… 🎶 A few years ago we used the #12DaysOfCRIPSR hashtag...detect nucleic acids.  On the third day of CRISPR, Addgene gave to me: tips and tricks for designing gRNAs...

  14. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ...no weak links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal...cover it. Addgene: OK, thanks for your time, and thanks for depositing your plasmids with Addgene. Howarth...lab at the University of Oxford is dedicated to generating new tools to manipulate biology based on molecular...based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce irreversible...another important step forward by dissecting that S. pyogenes protein into three parts. Their efforts yielded... from the “S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together... and its potential uses. Developing a new tool Addgene: Where did you get the idea to develop SpyLigase...

  15. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...reference, Addgene commonly prepares NGS samples using both pre- and post-amplification DNA and generates PCR...CRISPR Libraries Resources on Addgene.org Browse Pooled Libraries at Addgene ...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...million plasmids. These libraries are some of Addgene’s most exciting and versatile offerings! We recently...plasmids are amplified and none are lost. Each Addgene library has its own depositor transformation and...components of the library are represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing...transformation efficiency. For a typical library, Addgene usually does four to eight electroporation reactions...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...', style='' %} Resources on the Addgene blog Subscribe to the Addgene blog Read our viral vector blog...blog posts Learn more about Addgene Resources on the Addgene website Find plasmids for your research  ...Addgene has so much to celebrate! As we enter this new growth phase of the organization and the expansion...and the opportunities we have for the future. At Addgene we will never ever stop planning, perfecting, and...new projects. Diversification is happening. The Addgene team will have some fun challenges this year and...get excited about making their first deposit to Addgene. We are also lauded by another set of stakeholders...we received some entirely unsolicited praise: “Addgene, for one, is a sterling example of collaboration...

  17. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...first antibodies distributed by Addgene.  The plasmids used to generate these antibodies were based off... used to generate cDNA, which  was used as a template for PCR amplification with a degenerate primer set...Introduction to Antibodies Resources on Addgene.org  Browse Addgene's recombinant antibody plasmid collection...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the construction...et. al., Elife  A limitation of hybridoma generated mAbs is that if the frozen cell line becomes non-recoverable...grow up the cells. This RNA could then be used to generate functional recombinant monoclonal antibody (R-...

  18. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Antibody, Addgene gave to me… The Addgene Antibody Data Hub!    🎶On the sixth day of Antibody, Addgene gave...Antibody, Addgene gave to me…  An Antibodies 101 eBook!  🎶On the second day of Antibody, Addgene gave to...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave ...Antibody, Addgene gave to me… Antibodies developed by IPI!   🎶On the eighth day of Antibody, Addgene gave ...Antibody, Addgene gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody, Addgene gave to...Anti-DYKDDDDK!   🎶On the ninth day of Antibody, Addgene gave to me… A neuroscience antibody collection!...flow plots!   🎶On the twelfth day of Antibody, Addgene gave to me…  A growing recombinant antibody collection...

  19. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s catalog...imaging. Vladislav Verkhusha’s lab has generated a new genetically encoded calcium indicator using small...on the Addgene blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent...Here at Addgene’s headquarters, the skies are getting dark and wintry with the end of year approaching...other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted GFP into an...localized for synaptic glutamate response. They generated variants optimized for different applications ...St-Pierre lab and colleagues have developed a new genetically-encoded voltage indicator (GEVI) called JEDI-...

  20. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...learning.” Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since...work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt, Addgene’s Associate...resources on the Addgene blog Browse our science career blog posts Download Addgene's science career guide... guide Resources on Addgene.org Learn more about working at Addgene ...Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member...go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community...community college when she took her first job at Addgene as an Order Processing Assistant. After a few months...

  21. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...Resources on the Addgene Blog Learn About the Addgene Help Center Get Help with the Addgene Deposit Process...emails in my six years as a senior scientist at Addgene. Having spent that long in customer service, I'...Though my experience is based solely on my time at Addgene, I’m confident that these tips and tricks will ...another great source of information. At least at Addgene, the questions in our FAQ section are really, truly...not realize it, all of the reagents available at Addgene come from researchers, and the vast majority of...that for you” meme is infamous enough to have generated it’s own tongue-in-cheek website.  If you are ...happens more frequently than you might think. The Addgene scientists answering the phone are smart, but they...

  22. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...from addgene.org  Plasmids encoding nanobodies at Addgene Sybody Generation Toolbox from Addgene     ...only antibodies. (A) Overview of genetic modifications made to generate the nanomouse. (VHH = variable ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...to human populations around the world. Here at Addgene, we love camelids too, though not just for the ...chromobodies,” which are single-domain antibodies genetically fused to fluorescent proteins and used to visualize... synthetic single-domain antibody libraries to generate new sdAbs. There has also been some interesting...) (Xu, et al., 2021).      Figure 2: Genetically modified mice offer an alternative method to ...

  23. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    Published
    Aug. 27, 2020, 1:15 p.m.
    ...been generated for yeast (Kim et al., 2019) and fruit flies (Bischof et al., 2018), the generation of such...quantities (and the correct stoichiometric ratios) to generate useable data. What’s more, measuring and quantifying...straightforward nature of BiFC also makes it easier to generate a large number of constructs for high-throughput...screening of PPIs. For example, Bischof et al. (2018) generated an open reading frame (ORF) library to screen ... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...pair. Find BiFC plasmids for your experiment at Addgene! How to Design a BiFC Experiment Much like FRET...optimized to be successful. A BiFC experiment generally involves three steps: fusion protein construction...

  24. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...scientists are generous Besides some of the scientists I've already mentioned, Addgene depositors Marius...What an honor for Addgene to be a part of that!  Additional Resources on the Addgene Blog Learn about...landmark Cell paper showing that it was possible to generate induced pluripotent stem cells (iPSCs) capable...called OSKM factors). As the Yamanaka lab's primary Addgene contact for many years, I've been looking forward...example of the fast movement in this field.  As an Addgene scientist, I'm exposed to many scientific communities...clinical trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's...quality control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took...

  25. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...communication blog posts Resources on Addgene.org Find careers at Addgene ... project I’d seen in my already long tenure at Addgene. I would work with our research team and software...pinpointed and (mostly) solved two main problems with Addgene’s cross team communication. Although these real ...these plates now” to a fellow team member. But, at Addgene, we use seven different types of “plates” (Fig....not the container was barcoded (this is pretty Addgene specific) The types or purposes of each given container...that there were a lot of words that nearly all Addgene teams used that seemed simple, but they could in...solution for this problem was the creation of an Addgene lexicon. The base lexicon was and continues to ...

  26. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...approaches to control transgene expression by restricting expression to genetically defined cell types. ...neuroscientists have begun to use more precise genetic tools: activators, inhibitors, sensors, recombinases...vectors (AAV), neuroscientists can combine classical genetic tools with the manipulation of specific neuronal...changes affect the phenotype. Viral vectors and genetic approaches are continuously refined and developed...molecular tools and sensors Combinations of classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV.... Specific transgene expression can be also achieved in nontransgenic animals by using cis regulatory ... one location can result in expression of an optogenetic payload in neurons that project to different ...

  27. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...CRISPR, Addgene gave to me: a primer on prime editing.  On the eleventh day of CRISPR, Addgene gave to...all of them here.  On the first day of CRISPR, Addgene gave to me: a miniature CRISPR-Cas system for mammalian... mammalian use. On the second day of CRISPR, Addgene gave to me: engineered pegRNAs to improve prime editing...editing efficiency. On the third day of CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation ... Delivery (SEND). On the fourth day of CRISPR, Addgene gave to me: enhanced prime editing systems through...manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me: efficient CRISPR editing with a hypercompact...engineered guide RNAs. On the sixth day of CRISPR, Addgene gave to me: PRIME-del (Precise genomic deletions...

  28. New Viral Vectors - Fall 2024

    Type
    Blog Post
    Published
    Oct. 3, 2024, 1:15 p.m.
    ...preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the... AAV1 Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...other new AAV vectors as well — including next-generation biosensors for GABA, dopamine, serotonin, and...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV5 Optogenetics Ofer Yizhar New viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE...-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar New viral prep with multiple serotypes pAAV-Syn-FLEX-rc...

  29. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...analyze and annotate any DNA sequence. SnapGene Viewer: The free SnapGene Viewer is great for looking at plasmid... biology tools.  Addgene Data Hub: A data repository for validation data on Addgene/CLOVER viral vectors...Utah.   Figure 1: A plasmid map created in SnapGene of Blue Flame plasmid pET21a-BirA.   DNA / .../ protein database search and analysis tools Addgene's analyze sequence tool: Annotates features, BLASTs...restriction enzyme cut sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled...available sources, as well as those deposited with Addgene. NCBI BLAST: Compares nucleotide or protein sequences...tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence manipulation...

  30. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...C-terminal region of the human proto-oncogene c-Myc. Since the generation of the first anti-c-Myc [9E10] (...high-throughput screening platform to apply saturating mutagenesis on 21 positions in the protein sequence of the...approach and outcomes of the screen for improved genetically-encoded voltage indicators (GEVIs) based on the...kinetics. They demonstrate the use of HcKCR1 as an optogenetic silencer in experiments with mouse cortical neurons... HcKCR2, making it more suitable for in vivo optogenetic use.   Fig. 2: A) HcKCR1 is highly selective...control K+ gradients and can be used as a potent optogenetic silencer. The current work also proposes promising... light-gated potassium channels that mediate optogenetic inhibition. Nat Neurosci 25, 967–974 (2022). ...

  31. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...pAAV-hsyn-flex-Voltron-ST DREADDs / Chemogenetics AAV We have also expanded our inventory of chemogenetic AAV for activating...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional...FDA-approved drugs. See our inventory of DREADDs / Chemogenetics AAVs New DREADDs Plasmid Serotype Name ...AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  AAV5 ...pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors New optogenetics inhibitors Plasmid Serotype Name 99039 ...These vectors listed below should be released at Addgene in the next 6 months, pending quality control. ...

  32. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ... Here’s how Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!)...Streamlined Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or ... on collaboration (it’s the heart and soul of Addgene’s mission), but legal complexities can arise when...institutions require them for material transfers. When Addgene entered the plasmid sharing space, we heard over...confidence. Benefits of the UBMTA: Peace of mind Addgene has adopted the terms of the Uniform Biological... review by the Provider Limited liability Addgene makes it easy Even with all those benefits, we ... handle all the paperwork. Record Keeping: Addgene will let you know exactly how many times your materials...

  33. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...protocols Sep-OTSλ plasmid - Addgene # 68292 C321.ΔA bacterial strain – Addgene #68306 pCRT7 NT TOPO E17TAG...E17TAG GFP plasmid - Addgene #68295 SupD plasmid – Addgene # 68307 For further technical reference and protocols...candidate regulatory kinase is known, it’s possible to generate non-phosphorylated recombinant protein and perform...Creating phosphoserine-incorporated proteins The generation of pSer-incorporated proteins requires a molecular...flavors’ of Sep-OTS plasmids are available through Addgene, but we have worked most extensively worked with...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...position. Practical tips for your experiments To generate as much pSer-incorporated protein of interest ...

  34. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ... biology over the past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins. From considering protein... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

  35. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...executive director of Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work...thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed 188,729 materials to 39,844 scientists... three–year project that touched every team at Addgene. As a resource, it will allow scientists around...Institute for Protein Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed...focus on expanding international access to the Addgene repository, partially through work with our distributors...mention that 2024 is an incredibly special year for Addgene: it marks our 20th anniversary. Throughout the ...months, we’ll also share our work in evolving Addgene’s strategic vision for the future. Over the years...

  36. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...perfect job.   Resources at Addgene Read Additional Career Posts on the Addgene Blog Browse Our Careers ...a full time position in a lab. I worked on the genetics of Wilms Tumour, which is a type of pediatric ...study a Master of Science (MSc) in Human Molecular Genetics to give me an edge over other graduates during...costs. My MSc courses were interesting and luckily genetics is a sector which has been rapidly growing. These...factors left me optimistic that I would find a genetics related role soon after finishing my MSc. The ...Australia, this was a perfect opportunity to work in a genetics lab. As a UK citizen, I was entitled to a Work-Travel...the best outcome for a specific combination of genetic mutations. This is a particular challenge in pancreatic...

  37. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    .../nn.4265.  Additional resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids Visit... al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon...Although a wide range of fluorescent proteins and optogenetic tools exist, humans (like many organisms) are...magnetoresponsive fluorescent protein. Controlling an optogenetic or fluorescent tool with magnets would enable... molecules like proteins with magnetic fields generally appears to be weak — if they exist at all. Andrew... AsLOV2. After several rounds of semi-random mutagenesis and screening, the EGFP and mScarlet variants...significant gains! After a few more rounds of mutagenesis, they eventually found a version of AsLOV2 with...

  38. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...technique, you first perform a round of PCR to generate DNA fragments with 15-40 bp of homology at their... site, toposisomerase I, and a taq polymerase-generated insert. When mixed, topoisomerase I provides both...Taq perfectly anneal with the topoisomerase I generated overhangs in the backbone. Once combined, the ...processes will require a PCR reaction or two to generate templates for the cloning process. Time spent ...spent actually doing the experiments are also generally comparable so the best way to compare costs is to ...assembly is a highly versatile and efficient way to generate a wide variety of constructs. Similar, Gateway...$20-30 per reaction but can be used to quickly generate multiple clones. Difficulty: If you’re an experienced...

  39. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...the iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical...Archon1 can also be used in conjunction with optogenetic activators and inhibitors that respond to blue... Find Archon1 and its cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

  40. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics guide BRAIN initiative plasmid... on the Addgene blog Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors...electric stimulation, drug administration, and optogenetics. Enter neural fibers! The neural fiber tool...features enable the fibers to emit light for optogenetic purposes, measure temperature, study electrophysiology...molecules.   Fun fact: A 1 cm block of polymer can generate many meters of fibers!     Fig. 1 - ...the labs looked at previous implementation of optogenetic tools, calcium sensors, and other common neuroscience...for gut implantation. The fibers they ended up generating were 10-15 times more flexible than the brain...
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