We narrowed to 608 results for: Sis

1. Anatomy of a Plasmid Page at Addgene

   Type

   Blog Post

   Published

   Feb. 4, 2016, 3:30 p.m.

   ...is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are listed, as...

2. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   Published

   Sept. 24, 2015, 2:30 p.m.

   ...mismatch repair assay? Or is PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate...

3. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   Published

   July 16, 2015, 2:30 p.m.

   ...Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor at the University of Miami Miller School...

4. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   Published

   Dec. 12, 2013, 3:35 p.m.

   ...describing the development of “transcriptors,” transistor-like digital genetic switches that enable cellular...

5. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ...to directly deliver a ribonucleoprotein (RNP), consisting of the Cas9 protein in complex with a targeting...

6. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...Organization Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources...

7. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...five Addgene colleagues who read it with me, the consistent response from scientist and non-scientist alike...

8. Summer SciComm Series: Modes of Communication

   Type

   Blog Post

   Published

   July 1, 2022, 1:15 p.m.

   ...elephants than it is to design one demonstrating mitosis.   Consider also the amount and type of information...

9. Antibodies 101: Producing Recombinant Antibodies

   Type

   Blog Post

   Published

   Aug. 13, 2024, 1:15 p.m.

   ...At its core, harvesting recombinant antibodies consists of separating antibodies from media, cells, and...

10. Course-Based Undergraduate Research Experiences (CUREs)

    Type

    Blog Post

    Published

    Feb. 9, 2023, 2:15 p.m.

    ...course recently used a combination of phylogenetic analysis, structural prediction, and bench work to identify...

11. A Guide to Starting Your Own Journal Club

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    Blog Post

    Published

    Feb. 6, 2020, 3 p.m.

    ...recurring journal clubs on Immunology and Microbial Pathogenesis that occurred once a week for several months...

12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type

    Blog Post

    Published

    Aug. 9, 2016, 2:30 p.m.

    ...which of these 13,000 regions are critical? One hypothesis: the enhancers! This leads us to a three-step...

13. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    .... If you are going to complete your colony PCR analysis in the same day, you can save the leftover bacteria-water...

14. The Importance of a Fun Workplace: Company Culture at Addgene

    Type

    Blog Post

    Published

    Sept. 3, 2015, 2:30 p.m.

    ...made all the difference here at Addgene is the emphasis placed on team building throughout the years. ...

15. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type

    Blog Post

    Published

    Sept. 23, 2014, 3:05 p.m.

    ...deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...

16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...safe-targeting guides vs. non-targeting guides on screen analysis and hit calling. 5,644 non-targeting guides and...

17. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived...

18. Plasmids 101: Gateway Cloning

    Type

    Blog Post

    Published

    Jan. 12, 2017, 3:30 p.m.

    ...destination vector carry different antibiotic resistance markers (indicated here by plasmid color), allowing...

19. Lab to Office Culture Shock

    Type

    Blog Post

    Published

    Feb. 5, 2019, 1:04 p.m.

    ... I find that my vocabulary improves on a weekly basis. There will be meetings. And some more meetings...

20. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ...Integrations with additional tools are added on a regular basis, so the ecosystem of related tools is always growing...

21. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...JK, Been KW, Yoon S, Kim J-S (2016) Genome-wide analysis reveals specificities of Cpf1 endonucleases in...

22. Finding nucleic acids with SHERLOCK and DETECTR

    Type

    Blog Post

    Published

    April 16, 2020, 1 p.m.

    ...science.aas8836 Niewoehner O, Jinek M (2016) Structural basis for the endoribonuclease activity of the type III-A...

23. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ... Consider, for instance, a disease like cystic fibrosis, which can be caused by thousands of different...

24. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type

    Blog Post

    Published

    July 30, 2024, 1:15 p.m.

    ...Our viral vectors data is less amenable to such analysis, due to its inherent bias: Addgene uses a prioritization...

25. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ... research; however, microglia are notoriously resistant to viral transduction. Through in vivo screening...

26. Tips for Writing a Good Cover Letter

    Type

    Blog Post

    Published

    May 21, 2024, 1:30 p.m.

    ...department] at [name of the university].During my thesis work on [phenomenon X], I read several of your ...

27. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...Creative Commons license.  Histograms  For analysis of a single marker, a histogram showing frequency...

28. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ... giving you a call. Many companies enlist the assistance of recruiters for identifying appropriate job...

29. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...second-line team that takes on cases requiring in-depth analysis, data review, in-house testing etc. Technical ...

30. Changing Labor Laws Bring Increased Postdoc Wages

    Type

    Blog Post

    Published

    Oct. 25, 2016, 2:30 p.m.

    ...Washington) but as discussed elsewhere, there seems no basis for this decision in FLSA, and at the time of writing...

31. Healthcare Consulting: A Door to the Business of Life Sciences

    Type

    Blog Post

    Published

    Oct. 13, 2016, 2:30 p.m.

    ...fast turnover of projects, pressure to maintain consistently high levels of performance, and occasionally...

32. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...address a reviewer’s concerns, you are making your hypothesis stronger. When you share data at a conference...

33. Quick Guide to Near-Infrared Fluorescent Proteins

    Type

    Blog Post

    Published

    Oct. 5, 2021, 1:15 p.m.

    ...fluorescence changes occurring due to protein synthesis, degradation, or reconstitution (reviewed in Shcherbakova...

34. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type

    Blog Post

    Published

    June 3, 2021, 1:15 p.m.

    ...Targeting Application. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy 34:404–417 . https://doi....

35. Filming Science Videos in the Age of Social Distancing

    Type

    Blog Post

    Published

    May 20, 2021, 1:15 p.m.

    ...have, and we developed a great plan to start consistently knocking out high-quality videos for you all...

36. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ..., Gabrieli T, Lou C, Ebenstein Y, Zhu TF. Cas9-Assisted Targeting of CHromosome segments CATCH enables...

37. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type

    Blog Post

    Published

    April 5, 2018, 12:42 p.m.

    ...Gurumurthy, Masato Ohtsuka, and Rolen M. Quadros for assistance with editing this blog post. References Yang,...

38. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ... dead or dying cells so that you can base your analysis on living cells only (for example, propidium iodide...

39. CRISPR 101: Off-Target Effects

    Type

    Blog Post

    Published

    Feb. 8, 2024, 2:15 p.m.

    ...generate predictions of these outcomes. Rigorous analysis is essential to ensure off-target events are below...

40. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type

    Blog Post

    Published

    Jan. 16, 2025, 3:42 p.m.

    ...Aschauer, D. F., Kreuz, S., & Rumpel, S. (2013). Analysis of transduction efficiency, tropism and axonal...