We narrowed to 803 results for: Cre

...Figure 1. The RepCap plasmid and its products. Created with BioRender.com   Replication (rep) gene...

...mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM requirements, which...

...spinal cord neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that...

...restriction enzymes, but you will also be able to create custom annotations and design additional experiments...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum...

...plasmids to add the citation and release the hold. Increasing impact Making the plasmids available as soon ...

...genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular...

... foreign DNA into another cell. Minimally, lab-created plasmids have an origin of replication, selection...

...packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting for 40% of...

... the correct size (vector + insert). You should screen 3-4 colonies to ensure that you find the correct...

...find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ...

... of combining “new discoveries or technologies increase chances that your project will be fresh as well...

... paying attention to living a “green” life and creating less waste.  Most communities seem to have recycling...

...Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where...

... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

...genome engineers continue to work diligently and creatively to deliver a highly specific, programmable platform...

... intestines known as neuropods. They observed increased vagus nerve firing in response to both of these...

...FLAG, but can be more!) to a protein’s termini to increase detection. The tag can also be removed by treating...

...to bind to a previously hidden epitope (right). Created withBioRender.com.   Biological Processes  ...

...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...

...series of simulations and machine learning, they created a classifier that predicts whether a gene was natural...

...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change ...

...modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course, ...

...are under development. Today, ABRC does more than create and share resources, we seek out opportunities ...

...Plasmids 101 eBook! In keeping with our goal of creating a one-stop reference guide for plasmids we've ...

...Programs with CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize the concepts...

...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this...

... can help us to address? Boyden: There are two concrete areas of benefit: First, there is fundamental ...

... Anti-GFP [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   ...

...stigma around sexual and reproductive health issues creates conditions under which misinformation can flourish...

...breaks an important law of networking--don’t be creepy. There is a person on LinkedIn I once helped by...

...discuss the process, which eventually led to the creation of a standardized agreement on the transfer of...

...enthusiasm for actually making it happen would increase dramatically. I’m not sure what the best approach...

... among others. They additionally had to think creatively so as not to develop the most obvious strategy...

...EGFP).  Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs...

... skills, which are useful to many employers. Increasingly, computer skills are highly desired, as roles...

...these projects could also lead to novel yeast creations that may not be pursued in an academic or industry...

..., it's hard to get those words out and onto the screen and transform your work into a paper or a grant...

...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving...

...has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard...

...The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ...

...flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller...

...basic guidelines below, you’ll likely be able to create a small, usable panel.  First, fluorophores vary...

... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects...

...leadership approach to each person, I was able to create a more productive and cohesive team environment...

...cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound...

...signaling pathway.  As a proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway...

...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...