We narrowed to 812 results for: Cre

...facts. Note: The images used in this post were created using data from or modified from Ke et al. 2017...

...Filters → Available to Industry.” Figure 1: Screenshot of Addgene's search functionality. Fluorescent...

...support for our resources with our new Help Center! Created using the same reliable Zendesk system that powers...

...preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for both transient...

...of the job application process can dramatically increase the chances of being seriously considered for ...

...PAiRFP2, to the in vivo imaging toolbox, which increase their fluorescence upon illumination with far-...

...spinal cord neurons using the Gal4/UAS system. This created fish lines with different groups of neurons that...

...packaging Packaging is a huge contributor to the increase in plastic manufacturing, accounting for 40% of...

...Figure 1. The RepCap plasmid and its products. Created with BioRender.com   Replication (rep) gene...

...restriction enzymes, but you will also be able to create custom annotations and design additional experiments...

...mutating these sequences. Pro tip! Various labs have created Cas9 enzymes with looser PAM requirements, which...

...plasmids to add the citation and release the hold. Increasing impact Making the plasmids available as soon ...

...genomic DNA downstream of gRNA-specified sites. Created with BioRender.com. CASTs quickly became popular...

...resin. The MBP tag, although large, can actually increase expression and solubility of the tagged protein...

...immune response against a pathogen. The Fc domain creates a link between the antibody and other parts of ...

... the correct size (vector + insert). You should screen 3-4 colonies to ensure that you find the correct...

... foreign DNA into another cell. Minimally, lab-created plasmids have an origin of replication, selection...

... at eScience Labs, an educational company that creates hands-on science lab kits and digital curriculum...

...find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ...

... of combining “new discoveries or technologies increase chances that your project will be fresh as well...

... paying attention to living a “green” life and creating less waste.  Most communities seem to have recycling...

...Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where...

... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure...

...genome engineers continue to work diligently and creatively to deliver a highly specific, programmable platform...

...are under development. Today, ABRC does more than create and share resources, we seek out opportunities ...

...to bind to a previously hidden epitope (right). Created withBioRender.com.   Biological Processes  ...

...especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change ...

...organization we’ve become. Our new branding was created by our team of designers and shaped by feedback...

...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...

... intestines known as neuropods. They observed increased vagus nerve firing in response to both of these...

...FLAG, but can be more!) to a protein’s termini to increase detection. The tag can also be removed by treating...

...modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course, ...

...series of simulations and machine learning, they created a classifier that predicts whether a gene was natural...

...Plasmids 101 eBook! In keeping with our goal of creating a one-stop reference guide for plasmids we've ...

...Programs with CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize the concepts...

...would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this...

... can help us to address? Boyden: There are two concrete areas of benefit: First, there is fundamental ...

...enthusiasm for actually making it happen would increase dramatically. I’m not sure what the best approach...

...breaks an important law of networking--don’t be creepy. There is a person on LinkedIn I once helped by...

... among others. They additionally had to think creatively so as not to develop the most obvious strategy...

...EGFP).  Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs...

... Anti-GFP [N86/8R] page for more details. Image credit: Addgene.   Find anti-GFP [N86/8R] here!   ...

...stigma around sexual and reproductive health issues creates conditions under which misinformation can flourish...

... skills, which are useful to many employers. Increasingly, computer skills are highly desired, as roles...

...discuss the process, which eventually led to the creation of a standardized agreement on the transfer of...

...only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest...

..., it's hard to get those words out and onto the screen and transform your work into a paper or a grant...

...these projects could also lead to novel yeast creations that may not be pursued in an academic or industry...

...points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving...

...has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard...