We narrowed to 791 results for: Cre
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TypeBlog PostPublishedApril 15, 2014, 3:11 p.m.... paying attention to living a “green” life and creating less waste. Most communities seem to have recycling...
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Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m....Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where... -
Choosing a Good Mentor for Scientists
TypeBlog PostPublishedJan. 16, 2014, 2:56 p.m.... lunch or beer) and in 1:1 conversations. This creates a level of familiarity and confidentiality to ensure... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog PostPublishedNov. 25, 2013, 10:12 p.m....genome engineers continue to work diligently and creatively to deliver a highly specific, programmable platform... -
Viral Vectors 101: Transductions
TypeBlog PostPublishedApril 30, 2024, 1:15 p.m....for the transduction step. Viral particles are secreted into the cellular media, making viral collection... -
Antibodies 101: Epitope Tags
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....FLAG, but can be more!) to a protein’s termini to increase detection. The tag can also be removed by treating... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....to bind to a previously hidden epitope (right). Created withBioRender.com. Biological Processes ... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m.... intestines known as neuropods. They observed increased vagus nerve firing in response to both of these... -
Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation
TypeBlog PostPublishedNov. 8, 2018, 3:47 p.m....series of simulations and machine learning, they created a classifier that predicts whether a gene was natural... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....are under development. Today, ABRC does more than create and share resources, we seek out opportunities ... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....especially race and gender) Interpersonal issues can create interpersonal issues (one bad apple can change ... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course, ... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....Plasmids 101 eBook! In keeping with our goal of creating a one-stop reference guide for plasmids we've ... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog PostPublishedApril 7, 2015, 4:21 p.m....Programs with CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize the concepts... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....would be able to carry out a yeast-2-hybrid library screen; instead I thought they would focus on “Has this... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog PostPublishedMarch 4, 2014, 4:49 p.m.... can help us to address? Boyden: There are two concrete areas of benefit: First, there is fundamental ... -
The MTA at Addgene
TypeBlog PostPublishedJuly 23, 2024, 2:17 p.m....discuss the process, which eventually led to the creation of a standardized agreement on the transfer of... -
Hot Plasmids - January 2023
TypeBlog PostPublishedJan. 31, 2023, 2:15 p.m.... Anti-GFP [N86/8R] page for more details. Image credit: Addgene. Find anti-GFP [N86/8R] here! ... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m...., it's hard to get those words out and onto the screen and transform your work into a paper or a grant... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog PostPublishedJan. 12, 2021, 2:15 p.m....breaks an important law of networking--don’t be creepy. There is a person on LinkedIn I once helped by... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....stigma around sexual and reproductive health issues creates conditions under which misinformation can flourish... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m....enthusiasm for actually making it happen would increase dramatically. I’m not sure what the best approach... -
Cultivating Community Science at BosLab
TypeBlog PostPublishedNov. 2, 2017, 1:35 p.m....these projects could also lead to novel yeast creations that may not be pursued in an academic or industry... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m.... among others. They additionally had to think creatively so as not to develop the most obvious strategy... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....EGFP). Users can “favorite” certain proteins, or create custom protein collections (such as a set of FPs... -
Science Career Options
TypeBlog PostPublishedFeb. 23, 2017, 1:30 p.m.... skills, which are useful to many employers. Increasingly, computer skills are highly desired, as roles... -
5 Great Apps for Lab Life
TypeBlog PostPublishedSept. 22, 2016, 2:30 p.m....only need to skim the alphabet on the side of the screen to find the sequence for your enzyme of interest... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....cells but with far more predictability. The beads create an artificial positive (bound) and negative (unbound... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....The catch The rapid expansion of GCE led to the creation of many new tools and potential applications. ... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....flexibility of AAV genome design in remarkably creative ways. The continued development of more and smaller... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....basic guidelines below, you’ll likely be able to create a small, usable panel. First, fluorophores vary... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m.... IgG; but it's the most prevalent antibody in secretions (e.g. tears, saliva, mucus) where it protects... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog PostPublishedJan. 21, 2025, 2:15 p.m....leadership approach to each person, I was able to create a more productive and cohesive team environment... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m....signaling pathway. As a proof-of-principle, the team created an OptoNB to control the Ras/Erk signaling pathway... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m....many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog PostPublishedFeb. 10, 2016, 3:30 p.m....harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m....Genome-Wide Control for Large-Scale Functional Screens ... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....protein-protein interactions with other peptides. Created with BioRender.com. Many biologists can... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding... -
Visualizing Protein Turnover In Situ
TypeBlog PostPublishedJan. 16, 2018, 3:20 p.m....neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...